Article
Dentistry, Oral Surgery & Medicine
H. Zheng, Y. Shi, L. Bi, Z. Zhang, Z. Zhou, C. Shao, D. Cui, X. Cheng, R. Tang, H. Pan, Z. Wu, B. Fu
Summary: Methacryloyloxydecyl dihydrogen phosphate (MDP) has the ability to induce mineralization. Through various techniques, we demonstrate that MDP can demineralize dentin and infiltrate collagen fibrils to induce intrafibrillar mineralization. This dual functionality of MDP could have significant implications in adhesive dentistry and preventive dentistry.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Environmental Sciences
Tianyu Zhao, Iana Markevych, Marie Standl, Zhonglin Lyu, Tamara Schikowski, Dietrich Berdel, Sibylle Koletzko, Andrea von Berg, Joachim Heinrich
Summary: This study found that long-term exposure to ambient ozone was associated with decreased concentrations of bone turnover markers in German children. However, unless other studies confirm these results, the detrimental effects of ambient ozone on bone development in children should be interpreted cautiously.
ENVIRONMENTAL RESEARCH
(2022)
Article
Physiology
Zihan Xu, Chao Yang, Feng Wu, Xiaowen Tan, Yaxiu Guo, Hongyu Zhang, Hailong Wang, Xiukun Sui, Zi Xu, Minbo Zhao, Siyu Jiang, Zhongquan Dai, Yinghui Li
Summary: Ocn is synthesized by osteoblasts and has been found to regulate energy metabolism, testosterone synthesis, and brain development. However, its precise function in bone is not fully understood. Researchers often use Bglap and Bglap2 knockout mice to study the function of Ocn, but the compensatory mechanisms of Bglap3 when Bglap and Bglap2 are knocked out are unclear.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Monica Kakkar, Vivek Kapoor, S. K. Singla, R. K. Jethi
Summary: In an in vitro system utilizing collagen isolated from sheep tendons, fluoride was found to inhibit mineralization at lower concentrations and stimulate it at higher concentrations, while also inhibiting demineralization. At low concentrations, fluoride acted similarly to Mg(2+) in inhibiting mineralization, but at higher concentrations, it acted as crystal poisons to inhibit demineralization. However, unlike magnesium and pyrophosphate, fluoride at higher concentrations was found to stimulate rather than inhibit mineralization.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Harry C. Blair, Quitterie C. Larrouture, Irina L. Tourkova, Deborah J. Nelson, Steven F. Dobrowolski, Paul H. Schlesinger
Summary: This article reviews the unique properties of bone formation, focusing on the current understanding of mechanisms of bone mineral transport. The bone matrix has specialized mechanisms for continuous transport and deposition of mineral, which is absent in other connective tissues. The specialized proteins that regulate connective tissue structure and formation are surprisingly similar in mineralized and non-mineralized tissues.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Engineering, Biomedical
Xiaoyan Ren, Qi Zhou, Meiwand Bedar, David Foulad, Kelly X. X. Huang, Dillon Dejam, Natalie J. J. Dahan, Vasiliki Kolliopoulos, Brendan A. C. Harley, Justine C. C. Lee
Summary: The study investigates the effects of phosphate content in nanoparticulate mineralized collagen glycosaminoglycan (MC-GAG) on the microenvironment and osteoprogenitor differentiation. It is found that MC-GAG exhibits a temporal relationship with soluble phosphate, eluting early in culture and then absorbing, regardless of the differentiation of primary human mesenchymal stem cells (hMSCs). The intrinsic phosphate content of MC-GAG is sufficient to stimulate osteogenic differentiation of hMSCs without the addition of exogenous phosphate, and this process can be reduced but not eliminated by knocking down the sodium phosphate transporters PiT-1 or PiT-2. The contributions of PiT-1 and PiT-2 to MC-GAG-mediated osteogenesis are nonredundant but nonadditive.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Paper & Wood
Dan Ding, Kunling Liu, Yunlan Liu, Yanfeng Lu, Yonghua Lu, Yao Cheng, Jinhao Li, Guangxian Zhang, Fengxiu Zhang
Summary: A halogen-free and formaldehyde-free phosphorus-nitrogen synergistic flame retardant was synthesized and applied to cotton fabric. The results showed that the flame retardant had excellent protective effects and did not significantly affect the softness and breaking strength of the cotton fabric.
Article
Biochemistry & Molecular Biology
Mara Rubia Marques, Pedro Henrique Graciano de Assis, Patricia Santos Azeredo, Jaqueline Aguiar Fleury, Janaina Ribeiro Costa, Liana Silva Gomes, Danilo Silva Lima, Naiara Cristina de Souza Ribeiro, Manoel Francisco Biancardi, Fernanda Cristina Alcantara dos Santos
Summary: The results of this study indicate that aluminum intake during the neonatal phase can impair endochondral ossification by affecting epiphyseal cartilage and bone architecture.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2022)
Article
Food Science & Technology
Peter G. Chiarelli, Jinru Chen, Ronald B. Pegg, Kevin Mis Solval
Summary: This study investigated the effect of demineralization on the physicochemical properties of hydrolyzed collagen from cannonball jellyfish. Demineralization significantly reduced mineral content and increased protein content, resulting in enhanced gelling and thickening properties of the gelatin powders. Gelatin solutions prepared with demineralized powders showed higher strength and viscosity. This study highlights the importance of demineralization in producing functional gelatin powders from cannonball jellyfish.
Article
Biochemistry & Molecular Biology
Veronika Hefka Blahnova, Lucy Vojtova, Veronika Pavlinakova, Johana Muchova, Eva Filova
Summary: By modifying collagen I-based foams with calcined hydroxyapatite, researchers found that the resulting scaffold showed significant osteogenic potential. The scaffold promoted the expression of osteogenic markers and could be easily manipulated to suit different defect sizes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Behnam Bakhtiarimoghadam, Sadegh Shirian, Esmaeil Mirzaei, Siavash Sharifi, Iraj Karimi, Gelavizh Gharati, Sara Takallu, Hassan Nazari
Summary: The study aimed to investigate the effect of different hydrogel materials with or without mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on bone regeneration in rabbit models. The results showed that in situ HA/Co hydrogel seeded with MSC and enriched by PRP can accelerate bone regeneration by promoting osteoblastic production of osteocalcin protein. Therefore, this approach may be a promising new strategy for bone tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Yan Zhang, Pan Hao, Huilian Li, Bo Miao
Summary: The study found that ponicidin has beneficial effects on the proliferation and calcium deposition of MG-63 cells, making it a potential candidate for treating bone diseases such as osteoporosis.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Geochemistry & Geophysics
Paul V. Ullmann, Kristyn K. Voegele, Kenneth J. Lacovara
Summary: Recent experiments have shed light on the reactions that stabilize biomolecules during early diagenesis, but little is known about the impact of groundwater chemistry on molecular preservation in bones over time. To investigate this, the researchers conducted experiments simulating bone decay using different fluid compositions. They found that acidic conditions during early diagenesis can outweigh the preservative effects of stabilization reactions, while early-diagenetic permineralization can significantly slow down biomolecular decay. The results provide insights into the decay of biomolecules in bone and other tissues, and further experiments could yield important information about the role of early-diagenetic environments in controlling decay.
Article
Endocrinology & Metabolism
Sarah Seberg Diemar, Louise Lylloff, Maria Sode Ronne, Line Tang Mollehave, Malene Heidemann, Betina Heinsbaek Thuesen, Jesper Johannesen, Anders J. Schou, Steffen Husby, Niels Wedderkopp, Christian Molgaard, Niklas Rye Jorgensen
Summary: The study aimed to establish reference intervals for bone turnover markers in children and adolescents, showing that these markers vary with age and sex, with β-CTX and PINP being the most affected by these changes which reflect bone metabolism processes.
Review
Biotechnology & Applied Microbiology
Abhishek Indurkar, Rajan Choudhary, Kristaps Rubenis, Janis Locs
Summary: Bone is a composite material consisting of inorganic calcium phosphate and organic collagen. The mineralization of collagen plays a crucial role in the formation of bone and tooth. It has been found that synthetic calcium phosphate undergoes a transformation to apatite nanocrystals before entering the collagen gap zone. However, this transformation is hindered by a nucleation catalyst. Organic macromolecules and low-molecular-weight carboxylic compounds present in bone and tooth can interact with both apatite and collagen, controlling nucleation, crystal growth, and particle size. These compounds also prevent the transformation of calcium phosphate into apatite before entering the collagen gap zone. Therefore, understanding the role of these organic compounds is important in regulating collagen mineralization.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Marta S. Carvalho, Joao C. Silva, Christopher M. Hoff, Joaquim M. S. Cabral, Robert J. Linhardt, Claudia L. da Silva, Deepak Vashishth
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Biochemical Research Methods
Timothy P. Cleland, Elena R. Schroeter, Caitlin Colleary
Summary: The term "diagenetiform" is introduced to accurately describe the changes detected on ancient proteins due to burial, providing a new opportunity for top-down paleoproteomic studies.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemical Research Methods
Timothy P. Cleland, Julianne J. Sarancha, Christine A. M. France
Summary: Proteomic techniques have provided a more comprehensive understanding of protein diagenesis in archaeology and paleontology, revealing various proteins in addition to collagen. The correlation between total deamidation and wt% N suggests a potential impact on nitrogen signal in bulk analyses. Despite varying sample quality, the amino acid profiles still reflect expected collagen I percentages, allowing for valid single amino acid stable isotope analyses.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Biology
Stacyann Bailey, Grazyna E. Sroga, Betty Hoac, Orestis L. Katsamenis, Zehai Wang, Nikolaos Bouropoulos, Marc D. McKee, Esben S. Sorensen, Philipp J. Thurner, Deepak Vashishth
Article
Multidisciplinary Sciences
Caroline Solazzo, Jean Soulat, Timothy Cleland
Summary: Tortoiseshell, a proteinaceous material derived from marine turtles, was used to create luxurious items in the 17th and 18th centuries and continues to drive illegal poaching of marine turtles. Proteomics analysis has identified marker sequences that can be used for species identification and studying degradation patterns in archaeological tortoiseshell.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Chemistry, Analytical
G. Asher Newsome, Timothy P. Cleland
Summary: The concentric trace gas permeation tube diffuses chemical reagents to a central carrier gas stream for driving chemical reaction pathways and influencing gas-phase chemistry in various atmospheric pressure ionization sources for mass spectrometry. The tunable permeation through the reservoir-jacketed polymer membrane is used to add dopants to the electrospray plume and perform hydrogen-deuterium exchange on biomolecules. Water permeation is also utilized to humidify the sample gas stream.
ANALYTICAL CHEMISTRY
(2021)
Article
Evolutionary Biology
Xuankun Li, Emily Ellis, David Plotkin, Yume Imada, Masaya Yago, Jacqueline Heckenhauer, Timothy P. Cleland, Rebecca B. Dikow, Torsten Dikow, Caroline G. Storer, Akito Y. Kawahara, Paul B. Frandsen
Summary: The research team presents a new annotated genome assembly of Neomicropteryx cornuta, offering valuable insights into the early evolution of Amphiesmenoptera. The highly contiguous genome assembly provides important resources for studying ecological and evolutionary transitions in the group. The study demonstrates high recovery of universal single copy orthologs and provides genome annotation of 15,643 genes.
GENOME BIOLOGY AND EVOLUTION
(2021)
Article
Endocrinology & Metabolism
Bowen Wang, Zehai Wang, Atharva A. Poundarik, Mohammed J. Zaki, Richard S. Bockman, Benjamin S. Glicksberg, Girish N. Nadkarni, Deepak Vashishth
Summary: This study found that poor glycemic control in individuals with type 2 diabetes is associated with an increased risk of all fractures, while the use of metformin and DPP4 inhibitors is associated with a reduced risk of fractures. Insulin, thiazolidinediones, and meglitinides are associated with an increased risk of fractures.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biochemical Research Methods
Elena R. Schroeter, Timothy P. Cleland, Mary H. Schweitzer
Summary: Paleoproteomics aims to characterize proteins in specimens affected by time, providing biological information not obtainable through morphological study. While most analyses focus on historical, archeological, or relatively young samples, recent advancements are expanding the exploration of the Cenozoic fossil history.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Engineering, Biomedical
Timothy P. Cleland, Zehai Wang, Bowen Wang, Catalin R. Picu, Deepak Vashishth
Summary: The study found that the distal phalanges in bat wings are actually low density bones containing collagen and proteins associated with mineralization, rather than cartilage. Changes in proteins related to mineralization are correlated with alterations in mineral crystal thickness and alignment along the wing length.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Biochemical Research Methods
Timothy P. Cleland, Michael L. Power
Summary: Milk is an important source of nutrition for neonatal mammals, containing bioactive molecules that may impact their physiology and immune function. This study used mass spectrometry to characterize the milk proteomes of a Pongo pygmaeus and a Gorilla gorilla, finding changes in the proteomes across lactation. The study also identified changes in immunoglobulins and demonstrated differences between samples from different individuals.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Physics, Atomic, Molecular & Chemical
G. Asher Newsome, Timothy P. Cleland
Summary: A miniature permeation device is used to add controlled amounts of gas-phase dopant to the air around atmospheric pressure nanoflow electrospray sources, including both a pulled glass capillary needle in a custom mount and a commercial nano-liquid chromatography emitter in a commercial ion source housing. By adjusting the flow rate, positioning, and enclosure of the nanoflow emitter, the charge distribution of intact proteins sprayed at 300 nL/min can be shifted to lower charge states with increasing amounts of nitrogen doped with diethylamine, leading to increased signal abundance. The extent of the increase depends on the mass and isoelectric point of the proteins.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Physics, Applied
Stacyann Bailey, Atharva A. Poundarik, Grazyna E. Sroga, Deepak Vashishth
Summary: Through various experimental methods, we have demonstrated for the first time the impact of glycation on the bone protein Osteocalcin (OC), including its effect on bone's resistance to fracture and structural integrity, as well as the biological and mechanical functions of OC through cross-linking with other proteins. These findings contribute to a better understanding of the effects of OC and its modifications on bone fragility, and provide evidence for potential therapeutic targets for maintaining skeletal health.
APPLIED PHYSICS REVIEWS
(2023)
Article
Multidisciplinary Sciences
Molly L. Corder, Emanuel F. Petricoin, Yue Li, Timothy P. Cleland, Alexandra L. DeCandia, A. Alonso Aguirre, Budhan S. Pukazhenthi
Summary: This study analyzed the serum metabolomes of captive black rhinos and found differences in metabolite profiles between subspecies, sex, and health status. The results indicate metabolic dysregulation and involvement of specific metabolic pathways in disease syndromes among captive black rhinos.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Timothy P. Cleland, Michael L. Power
Summary: This study used mass spectrometry to analyze the milk proteomes of orangutans and gorillas, finding changes in various proteins across lactation and identifying species/individual differences in samples from 8 to 12 months. This research serves as a baseline for other zoo-based milk studies.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
