Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Md Shaffiulah Shajib, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: This study evaluated the ability of a BMP inhibitor, LDN 193189, to prevent BMSC hypertrophy. The results showed that LDN 193189 did not prevent hypertrophy, but it was effective in countering mineralization and adipogenesis.
Article
Biochemistry & Molecular Biology
Natalie Vallant, Bynvant Sandhu, Karim Hamaoui, Maria Prendecki, Charles Pusey, Vassilios Papalois
Summary: This study compared the paracrine effects of MSC supernatants from different age and genotype sources on macrophages, revealing distinct patterns of inflammatory marker expression. These findings are crucial for preclinical and clinical studies utilizing MSCs for inflammation and allo-response mitigation in transplant patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Sarah Hochmann, Kristy Ou, Rodolphe Poupardin, Michaela Mittermeir, Martin Textor, Salaheddine Ali, Martin Wolf, Agnes Ellinghaus, Dorit Jacobi, Juri A. J. Elmiger, Samantha Donsante, Mara Riminucci, Richard Schaefer, Uwe Kornak, Oliver Klein, Katharina Schallmoser, Katharina Schmidt-Bleek, Georg N. Duda, Julia K. Polansky, Sven Geissler, Dirk Strunk
Summary: Multipotent stromal cells are attractive sources for cell therapy and tissue engineering, but their application is limited due to functional diversity. In this study, we compared the regenerative potential of different stromal cell types in vitro and in vivo and found that only bone marrow stromal cells (BMSCs) could fully regenerate critical-size femoral defects. This ability was attributed to cell type-specific epigenetic landscapes controlling transcriptional stromal differentiation networks. BMSCs initiated defect healing and eventually contributed to tissue remodeling.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ryan A. Denu, Peiman Hematti
Summary: MSCs have great potential for cellular therapy but are limited by tissue scarcity, requiring ex vivo expansion. Expansion and aging are associated with oxidative stress, highlighting the need for strategies to reduce it.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Cell & Tissue Engineering
Mohammad Saeed Kahrizi, Elnaz Mousavi, Armin Khosravi, Sara Rahnama, Ali Salehi, Navid Nasrabadi, Farnoosh Ebrahimzadeh, Samira Jamali
Summary: Mesenchymal stem/stromal cells (MSCs) have the potential to be used in regenerative medicine due to their self-renewal ability and ability to differentiate into multiple lineages. MSCs also secrete various mediators that play a complex role in modulating immune responses and promoting angiogenesis. However, MSCs may lose their biological function after being collected and expanded in vitro, and they may encounter a harsh environment after transplantation due to the lack of proper tensegrity structure between cells and the matrix. Therefore, pre-conditioning of MSCs is suggested to enhance their performance in vivo, leading to improved transplantation efficacy in regenerative medicine. In this review, we provide an overview of pre-conditioning methods that can enhance the therapeutic efficacy of MSCs in organ failures, particularly in the kidney, heart, lung, and liver.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Andromachi Pouikli, Monika Maleszewska, Swati Parekh, Ming Yang, Chrysa Nikopoulou, Juan Jose Bonfiglio, Constantine Mylonas, Tonantzi Sandoval, Anna-Lena Schumacher, Yvonne Hinze, Ivan Matic, Christian Frezza, Peter Tessarz
Summary: This study reveals the impact of normal oxygen levels on the differentiation of bone-derived mesenchymal stem cells. High oxygen concentration promotes chromatin compaction and histone hypo-acetylation, resulting in osteogenic defects. Additionally, decreased activity of citrate carrier leads to the accumulation of acetyl-CoA inside mitochondria. Restoring cytosolic acetyl-CoA levels rescues the osteogenic defects.
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Cell & Tissue Engineering
Davod Pashoutan Sarvar, Hosein Effatpanah, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: The study highlights the important role of MSC-derived extracellular vesicles (MSC-EVs) in hematopoietic regulation and immune response after transplantation. MSC-EVs directly fuse with hematopoietic stem cell membranes, affecting their fate, and regulate the proliferation and differentiation of hematopoietic stem cells through the release of various bioactive factors.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Guanyin Chen, Dong Fan, Wangqian Zhang, Shuning Wang, Jintao Gu, Yuan Gao, Lei He, Weina Li, Cun Zhang, Meng Li, Yingqi Zhang, Zhaohui Liu, Qiang Hao
Summary: This study found that under hypoxic conditions, there was a significant increase in the expression of Mkx in MSCs. Mkx mediated hypoxia-induced tenogenic differentiation of MSCs but could not completely repress the proliferation of hypoxic MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Environmental Sciences
Hana Najahi, Nicola Alessio, Tiziana Squillaro, Gea Oliveri Conti, Margherita Ferrante, Giovanni Di Bernardo, Umberto Galderisi, Imed Messaoudi, Sergio Minucci, Mohamed Banni
Summary: This study evaluated the effects of different size microplastics present in water bottles on human mesenchymal stromal cells. The results showed that microplastic treatments led to a decrease in cell proliferation, altered differentiation potential, and loss of osteocyte phenotype. This indicates that environmental microplastics can be bioavailable for cell uptake and may result in irreversible diseases.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ines Moniz, Joao Ramalho-Santos, Ana F. Branco
Summary: This study demonstrates that different oxygen tensions have distinct effects on umbilical cord-mesenchymal stem cells (UC-MSCs), including changes in proliferation and metabolism. The use of CoCl2 as a substitute for hypoxic chambers yields different metabolic effects compared to gas-based strategies. Severe hypoxia modulates survival-related mechanisms through different mTOR complexes. This research provides valuable insights into the establishment of a hypoxia model for UC-MSCs and how different strategies for modulating hypoxia may impact experimental outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Chenghai Li, Bin Wang
Summary: Fibrosis refers to the connective tissue deposition and stiffness usually caused by injury, with tissue-resident mesenchymal cells playing a significant role in fibrogenic processes. MSCs may be a potential therapeutic option for fibrosis resolution, but their functional benefits in translational applications need to be carefully balanced with their potential risks.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Veterinary Sciences
Benjamin Uberti, Anita Plaza, Claudio Henriquez
Summary: Mesenchymal stem/stromal cells (MSCs) therapy is an important part of regenerative medicine and can modulate immune and inflammatory responses. However, there are discrepancies between the effects of MSCs in clinical applications and the results in experimental models, possibly due to limited survival or impaired function of cells in the inflammatory environment. Pre-conditioning strategies can enhance the survival and efficacy of MSCs.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Multidisciplinary Sciences
Alicia Sanchez-Mata, Elena Gonzalez-Munoz
Summary: This review outlines the origin, cell identity markers, and immunomodulatory properties of MenSCs, aiming to enhance understanding of their characteristics and identify potential avenues for exploration.
Article
Cell & Tissue Engineering
Yijun Liu, Nathalie Munoz, Bruce A. Bunnell, Timothy M. Logan, Teng Ma
Article
Cell & Tissue Engineering
Ang-Chen Tsai, Yijun Liu, Xuegang Yuan, Teng Ma
TISSUE ENGINEERING PART A
(2015)
Article
Biotechnology & Applied Microbiology
Teng Ma, Ang-Chen Tsai, Yijun Liu
BIOCHEMICAL ENGINEERING JOURNAL
(2016)
Article
Biotechnology & Applied Microbiology
Ang-Chen Tsai, Yijun Liu, Teng Ma
BIOCHEMICAL ENGINEERING JOURNAL
(2016)
Article
Cell & Tissue Engineering
Yijun Liu, Nathalie Munoz, Ang-Chen Tsai, Timothy M. Logan, Teng Ma
Article
Biochemical Research Methods
Ang-Chen Tsai, Yijun Liu, Xuegang Yuan, Ravindran Chella, Teng Ma
BIOTECHNOLOGY JOURNAL
(2017)
Article
Biotechnology & Applied Microbiology
Nathalie Munoz, Junho Kim, Yijun Liu, Timothy M. Logan, Teng Ma
JOURNAL OF BIOTECHNOLOGY
(2014)
Article
Thermodynamics
Xiao-Sen Li, Yi-Jun Liu, Zhi-Yong Zeng, Zhao-Yang Chen, Gang Li, Hui-Jie Wu
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2011)
Article
Cell & Tissue Engineering
Sebastien Sart, Yijun Liu, Teng Ma, Yan Li
TISSUE ENGINEERING PART A
(2014)