Article
Pharmacology & Pharmacy
Filippo Silva, Leopoldo Sitia, Raffaele Allevi, Arianna Bonizzi, Marta Sevieri, Carlo Morasso, Marta Truffi, Fabio Corsi, Serena Mazzucchelli
Summary: Protein nanocages are promising candidates in nanoscaled delivery systems, but concerns about endotoxin contamination in their production have prompted the development of a combined protocol for effectively removing endotoxins while maintaining high purity and protein recovery rates. This protocol, involving affinity purification with Endotrap HD resin and treatment with Triton X-114, has demonstrated success in maintaining nanocage interactions with target cells and specific recognition of cancer cells, paving the way for potential clinical trials.
Article
Chemistry, Multidisciplinary
Meng Gao, Xi Liu, Zhenzhen Wang, Hui Wang, Tristan Asset, Di Wu, Jun Jiang, Qianqian Xie, Shujuan Xu, Xiaoming Cai, Jia Li, Weili Wang, Huizhen Zheng, Xingfa Gao, Nikolai Tarasenko, Benjamin Rotonnelli, Jean-Jacques Gallet, Frederic Jaouen, Ruibin Li
Summary: This study proposes a mechanism for detoxifying endotoxins by engineering a dephosphorylation reaction using a synthetic nanozyme. The synthesized CeO2 effectively catalyzed the breakdown of P-O bonds in endotoxins, resulting in the attenuation of toxicity. Additionally, the CeO2 column exhibited a significantly higher treatment capacity compared to commercial resins.
Review
Medicine, General & Internal
Jakub Smiechowicz
Summary: Lipopolysaccharide, the main component of Gram-negative bacteria, is a potent toxin that can cause organ dysfunction in sepsis. Various pathways are involved in sensing endotoxin, and non-canonical activation of caspase-mediated pyroptosis is believed to play a major role in sepsis pathophysiology. Although anti-endotoxin therapies are considered as treatment options, there is currently not enough evidence to support their effectiveness. Further research is needed to evaluate the removal of endotoxin in COVID-19 patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemical Research Methods
Lucija Rebula, Andrej Raspor, Mojca Bavcar, Ales Strancar, Maja Leskovec
Summary: Bacteriophages have great potential as therapeutic agents, particularly in human therapy and antibiotic replacement. However, phage lysates may contain harmful by-products, making purification crucial. This article presents a two-step chromatographic purification method for P. aeruginosa bacteriophage, achieving high recovery rates and reducing contaminants. An HPLC analytical method was also developed for accurate measurement and analysis of phage concentration and impurities.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Sanchita Bhadra, Vylan Nguyen, Jose-Angel Torres, Shaunak Kar, Stephane Fadanka, Chiara Gandini, Harry Akligoh, Inyup Paik, Andre C. Maranhao, Jenny Molloy, Andrew D. Ellington
Summary: The study developed cellular reagents that can replace commercial pure enzymes in diagnostic and molecular biology reactions, and a simplified method for preparing these reagents using a common bacterial incubator and inexpensive chemical desiccants. The dried cellular reagents were shown to be effective in various biological processes, including PCR and DNA assembly, and successfully produced locally in countries such as the United Kingdom, Cameroon, and Ghana. The results demonstrate the potential for scalable local and distributed reagent production and highlight the opportunities available through synthetic biology.
Article
Multidisciplinary Sciences
Yuki Takahashi, Masatoshi Saito, Haruo Usuda, Tsukasa Takahashi, Shimpei Watanabe, Takushi Hanita, Shinichi Sato, Yusaku Kumagai, Shota Koshinami, Hideyuki Ikeda, Sean Carter, Erin L. Fee, Lucy Furfaro, Sylvain Chemtob, Jeffrey Keelan, David Olson, Nobuo Yaegashi, John P. Newnham, Alan H. Jobe, Matthew W. Kemp
Summary: This study investigated the anti-inflammatory efficacy of rytvela in a preterm sheep model of chorioamnionitis, showing that rytvela reduced LPS-induced inflammation and partially inhibited the expression of cytokines/chemokines in fetal tissues undergoing an active inflammatory response.
Article
Nanoscience & Nanotechnology
Sidharth Razdan, Justin Adler, Dibbya Barua, Sutapa Barua
Summary: The study aims to develop a multifunctional biofilter using PolyBall nanoparticles for efficient removal of endotoxins, cyanotoxins, and toxic heavy metal ions from water.
ACS APPLIED BIO MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Hongxu Sun, Hongrui Wang, Qiwei Chen, Wenge Dong, Chao Gao, Haiyan Song, Hui Peng, Ren Li, Hao Wu, Liangyu Hou, Yanhong Chang, Hui Luo
Summary: Protein purification is a fundamental technique in biological research and industrial production, and there is a constant pursuit of efficient, convenient, economical, and environmentally friendly methods. This study discovered that certain cations, including alkaline earth metals, alkali metals, and even nonmetals, can precipitate multi-histidine-tagged proteins at low salts concentrations. Based on this finding, a novel cation affinity purification method was developed that requires only three centrifugal separations and achieves high purity similar to immobilized metal affinity chromatography. The study also provides insights into unexpected protein precipitation and highlights the importance of considering the influence of cations on experimental results. The interaction between histidine-tagged proteins and cations may have broad application prospects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Michihiko Tsushima, Shinichi Sato, Kazuki Miura, Tatsuya Niwa, Hideki Taguchi, Hiroyuki Nakamura
Summary: Intracellular photocatalytic-proximity labeling (iPPL) is a method used to analyze protein-protein interactions in the microenvironment of living cells. Acriflavine was found to be an efficient cell-membrane-permeable photocatalyst for iPPL experiments. iPPL was applied to study the histone-associated protein H2B in cells, and it was found that proteins interacting directly with histones and RNA-binding proteins could be selectively labeled.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Udochukwu Camillius Ejike, Chong Joo Chan, Crystale Siew Ying Lim, Renee Lay Hong Lim
Summary: The study compared the functional differences between yFIP-Lrh produced in Pichia pastoris and eFIP-Lrh produced in E. coli. The results showed that yFIP-Lrh induced higher levels of Th1 and Th2 cytokines in mouse splenocytes and exhibited greater cytotoxic effects on cancer cells compared to eFIP-Lrh, possibly due to different post-translational modifications in the proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Junjia Luan, Lei Wang, Junnan Yang, Xuepeng Li, Jinxiang Wang, Fangchao Cui, Hongbo Mi, Yongxia Xu, Shumin Yi, Jianrong Li
Summary: Novel antioxidant peptides were identified from Pacific saury, and their structures and properties were analyzed. These peptides showed strong antioxidant activity and protective effects against oxidative damage in cells.
Article
Biology
Samsuzzoha Mondal, Honey Priya James, Francesco Milano, Rui Jin, Tobias Baumgart
Summary: BAR proteins, such as Amphiphysin, are known for generating membrane curvature during endocytosis. We successfully expressed and purified recombinant Amphiphysin and its N-BAR domain using affinity chromatography and GST tags. The cleavage of the GST tag in the N-BAR domain caused precipitation, which was minimized by adding glycerol to the purification buffers. Size exclusion chromatography was used to remove any oligomeric species. This protocol can also be applied to purify other N-BAR proteins.
Article
Chemistry, Multidisciplinary
Samuel L. Scinto, Tyler R. Reagle, Joseph M. Fox
Summary: This study demonstrates a new method for site-selective functionalization of proteins using pyridyl-tetrazine tags, allowing for direct affinity purification on commonly used nickel-iminodiacetate (Ni-IDA) resins. The method is also applicable for protein purification from complex mixtures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemical Research Methods
Rebecca Elizabeth Kattan, Deena Ayesh, Wenqi Wang
Summary: During intracellular signal transduction, protein-protein interactions (PPIs) play a critical role in regulating protein localization and function. Mass spectrometry-based interactome analysis has become a popular method for studying PPI networks, but the analysis of large datasets can be challenging. In this review, we discuss the methods and resources commonly used for analyzing large interactome-related proteomic data and propose a guideline for identifying novel interacting proteins.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Biochemical Research Methods
Roxana Roshankhah, Kyle Jackson, Truong Thu Ngoc Nguyen, Robert Pelton, Zeinab Hosseinidoust, Raja Ghosh
Summary: As cases of multidrug resistant bacterial infections increase, scientists and clinicians are turning to bacteriophages as alternatives to antibiotics. Researchers have developed a rapid, scalable, and efficient membrane chromatographic method for purifying bacteriophages, which removes impurities and ensures a high phage count in the purified samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Anne Serdakowski London, Kunal Patel, Lisa Quinn, Martin Lemmerer
PROTEIN EXPRESSION AND PURIFICATION
(2015)
Meeting Abstract
Oncology
Scott D. Collins, Parmita Saxena, Xiao Y. Li, Yeonju Shim, Lance Ostrom, Nicholas C. Yoder, Kalli C. Catcott, Molly A. McShea, Xiuxia Sun, Sanela Bilic, William R. Tschantz, Meghan Flaherty, Keith Mansfield, Tiancen Hu, Vladimir Capka, Markus Kurz, Ivana Liric Rajlic, Anne Serdakowski London, Duc Nguyen, Rebecca Mosher, Matthew J. Meyer, Aaron Bourret, Jamal Saeh, Scott Cameron, Emma Lees, Carl U. Bialucha
Article
Immunology
Anne Serdakowski London, Chietara Japutra, Kyle Planck, Michael Lihon, Andrew Anh Nguyen
INTERNATIONAL IMMUNOPHARMACOLOGY
(2016)
Article
Oncology
Carl U. Bialucha, Scott D. Collins, Xiao Li, Parmita Saxena, Xiamei Zhang, Clemens Durr, Bruno Lafont, Pierric Prieur, Yeonju Shim, Rebecca Mosher, David Lee Lance Ostrom, Tiancen Hu, Sanela Bilic, Ivana Liric Rajlic, Vladimir Capka, Wei Jiang, Joel P. Wagner, GiNell Elliott, Artur Veloso, Jessica C. Piel, Meghan M. Flaherty, Keith G. Mansfield, Emily K. Meseck, Tina Rubic-Schneider, Anne Serdakowski London, William R. Tschantz, Markus Kurz, Duc Nguyen, Aaron Bourret, Matthew J. Meyer, Jason E. Faris, Mary J. Janatpour, Vivien W. Chan, Nicholas C. Yoder, Kalli C. Catcott, Molly A. McShea, Xiuxia Sun, Hui Gao, Juliet Williams, Francesco Hofmann, Jeffrey A. Engelman, Seth A. Ettenberg, William R. Sellers, Emma Lees
Article
Physiology
Jennifer R. Peters-Hall, Melissa L. Coquelin, Michael J. Torres, Ryan LaRanger, Busola R. Alabi, Sei Sho, Jose F. Calva-Moreno, Philip J. Thomas, Jerry W. Shay
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2018)
Article
Cell & Tissue Engineering
Ryan LaRanger, Jennifer R. Peters-Hall, Melissa Coquelin, Busola R. Alabi, Christopher T. Chen, Woodring E. Wright, Jerry W. Shay
TISSUE ENGINEERING PART A
(2018)
Article
Biochemical Research Methods
Anne Serdakowski London, Brendan Kerins, William R. Tschantz, Kasey Mackay
BIOTECHNOLOGY JOURNAL
(2012)
Article
Biochemical Research Methods
Martin Lemmerer, Anne Serdakowski London, Alexandra Panicucci, Cristina Gutierrez-Vargas, Michael Lihon, Philippe Dreier
JOURNAL OF IMMUNOLOGICAL METHODS
(2013)
Article
Engineering, Biomedical
Busola R. Alabi, Ryan LaRanger, Jerry W. Shay
ACTA BIOMATERIALIA
(2019)
Article
Chemistry, Medicinal
Kris White, Matthew Esparza, Jue Liang, Prasanna Bhat, Jacinth Naidoo, Briana L. McGovern, Michael A. P. Williams, Busola R. Alabi, Jerry Shay, Hanspeter Niederstrasser, Bruce Posner, Adolfo Garcia-Sastre, Joseph Ready, Beatriz M. A. Fontoura
Summary: An inhibitor of the HA protein has been identified in this study, which prevents entry and replication of influenza viruses, showing potent antiviral activity against various subtypes. Important moieties for antiviral activity have been identified through synthesis of derivatives of this compound, suggesting its potential as a lead for drug development to enhance pan-subtype efficacy in combination with other influenza A virus antivirals.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sara Sepe, Francesca Rossiello, Valeria Cancila, Fabio Iannelli, Valentina Matti, Giada Cicio, Matteo Cabrini, Eugenia Marinelli, Busola R. Alabi, Alessia di Lillo, Arianna Di Napoli, Jerry W. Shay, Claudio Tripodo, Fabrizio d'Adda di Fagagna
Summary: As individuals age, the expression of ACE2 in the lungs increases, making elderly individuals more susceptible to SARS-CoV-2. Telomere damage triggers DDR activation, leading to an upregulation of ACE2 and potentially increasing susceptibility to infection in the elderly.
Article
Oncology
Shiqin Liu, Busola Ruth Alabi, Qingqing Yin, Tanya Stoyanova
Summary: Prostate cancer is a common cancer in men in the United States and often leads to cancer-related deaths. Androgen deprivation therapy is the standard treatment for advanced prostate cancer, but some patients develop castration-resistant prostate cancer and even neuroendocrine prostate cancer. Neuroendocrine prostate cancer is characterized by the expression of neuroendocrine markers and the absence of androgen receptor, and is often associated with aggressive tumors, therapy resistance, and poor clinical outcomes.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Cell Biology
Meghan A. Rice, Vineet Kumar, Dhanir Tailor, Fernando Jose Garcia-Marques, En-Chi Hsu, Shiqin Liu, Abel Bermudez, Vijayalakshmi Kanchustambham, Vishnu Shankar, Zintis Inde, Busola Ruth Alabi, Arvind Muruganantham, Michelle Shen, Mallesh Pandrala, Rosalie Nolley, Merve Aslan, Ali Ghoochani, Arushi Agarwal, Mark Buckup, Manoj Kumar, Catherine C. Going, Donna M. Peehl, Scott J. Dixon, Richard N. Zare, James D. Brooks, Sharon J. Pitteri, Sanjay V. Malhotra, Tanya Stoyanova
Summary: SU086 is identified as a potential treatment for advanced prostate cancer, either as a single agent or in combination with second-generation anti-androgens. It inhibits prostate cancer cell growth and decreases heat shock protein 90 (HSP90) levels, while perturbing glycolysis.
CELL REPORTS MEDICINE
(2022)
