Article
Biotechnology & Applied Microbiology
Carolina Ehrenfeld, Valentina Veloso-Gimenez, Rocio Corrales-Orovio, Rolando Rebolledo, Mauricio P. Boric, Jose Tomas Egana
Summary: As animal cells cannot produce oxygen, erythrocytes are responsible for gas interchange, being able to capture and deliver oxygen upon tissue request. Several other cells in nature produce oxygen by photosynthesis, raising the question of whether they could circulate within the vascular networks, acting as an alternative source for oxygen delivery. This study examines the physical and mechanical features of the photosynthetic microalga Chlamydomona reinhardtii and compares them with erythrocytes, finding similarities in size and rheological properties. Furthermore, the biocompatibility of the microalgae is evaluated, showing that they can co-culture with endothelial cells without affecting each other's morphology and viability. Additionally, the systemic injection of high numbers of microalgae in mice does not trigger harmful responses, suggesting their potential as a source of oxygen delivery.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mei-Li Zhao, Xiang-Yu Li, Cheng-Xiang Lan, Zi-Ling Yuan, Jia-Lin Zhao, Ying Huang, Zhang-Li Hu, Bin Jia
Summary: A synthetic pathway for ginsenosides was established in Chlamydomonas reinhardtii by introducing genes from Panax ginseng, resulting in high production of dammarenediol-II. Strategies such as gene loading and culture optimizing were employed to enhance productivity. This study provides a good platform for the further production of ginsenosides in microalgae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Monika Majewska, Darya Harshkova, Wojciech Pokora, Agnieszka Bascik-Remisiewicz, Szymon Tulodziecki, Anna Aksmann
Summary: Research found that DCF and atrazine had different effects on Chlamydomonas reinhardtii. Both substances decreased photosynthetic vitality of the cells, but for different reasons. Atrazine disrupted photosynthetic electron transport, leading to excessive ROS production and limited cell growth, while DCF silenced PSII reaction centers, preventing significant ROS overproduction.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Electrochemistry
Adnan Sayegh, Luca A. Perego, Marc Arderiu Romero, Louis Escudero, Jerome Delacotte, Manon Guille-Collignon, Laurence Grimaud, Benjamin Bailleul, Frederic Lemaitre
Summary: This study focuses on extracting energy from oxygenic photosynthesis by rerouting electron flow using intact photosynthetic organisms to generate photocurrent. Researchers found that some quinones can interact with the embedded photosynthetic chain, but the balance between toxicity and bioelectricity production needs to be considered.
Article
Chemistry, Physical
Huidan Xue, Yibei Dong, Zhihuan Li, Jing Wang, Xiaolong Yuan, Fei He, Zhengke Li, Xiang Gao, Jianxi Liu
Summary: Carbon dots have the ability to enhance photosynthesis in microalgae, promoting cell division and biomass. The red-emitting carbon dots improved the energy transfer and efficiency of photosynthesis, resulting in increased protein and lipid contents. Transcriptome analysis revealed that the carbon dots up-regulated genes associated with cell growth and energy conversion.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Valentina Veloso-Gimenez, Rosalba Escamilla, David Necunir, Rocio Corrales-Orovio, Sergio Riveros, Carlo Marino, Carolina Ehrenfeld, Christian Dani Guzman, Mauricio P. Boric, Rolando Rebolledo, Jose Tomas Egana
Summary: This study aimed to develop a perfusable photosynthetic solution with microalgae embedded to supply oxygen and support ex vivo organ preservation. The research found that the photosynthetic solution did not harm animal cells and provided sufficient oxygen to meet metabolic demands. Experimental validation showed that during organ perfusion, the photosynthetic microorganisms could reach the renal vasculature without causing tissue damage, surviving the process successfully.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biology
Peter Neofotis, Joshua Temple, Oliver L. Tessmer, Jacob Bibik, Nicole Norris, Eric Pollner, Ben Lucker, Sarathi M. Weraduwage, Alecia Withrow, Barbara Sears, Greg Mogos, Melinda Frame, David Hall, Joseph Weissman, David M. Kramer
Summary: The study shows that in Chlamydomonas reinhardtii, the pyrenoid can be strongly induced by hyperoxia, even in the presence of high levels of CO2 or bicarbonate. This suggests that the pyrenoid may be induced by a common product of photosynthesis specific to low CO2 or hyperoxia conditions. Additionally, evidence is presented for linkages between genetic variations in hyperoxia tolerance, H2O2 signaling, and pyrenoid morphologies.
Article
Chemistry, Multidisciplinary
Srikkanth Balasubramanian, Kui Yu, Anne S. Meyer, Elvin Karana, Marie-Eve Aubin-Tam
Summary: The study introduces a bioprinting technique for encapsulating microalgae within an alginate hydrogel matrix, resulting in materials with exceptional mechanical strength and stability. These bioprinted materials can detach and reattach onto different surfaces, survive without nutrients for at least 3 days, and be regenerative for future use. The potential product applications of these bioprinted living materials include artificial leaves, photosynthetic bio-garments, and adhesive labels, with the possibility of upscaling production.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Plant Sciences
Yaqi An, Dong Wang, Jingxia Du, Xinwei Wang, Jianwei Xiao
Summary: The carbon dioxide emitted by human accounts for only a small fraction of global photosynthesis consumption, half of which is due to microalgae. The high efficiency of algae photosynthesis is attributed to the pyrenoid-based CO2-concentrating mechanism (CCM). The formation of pyrenoid which has a variety of Rubisco-binding proteins mainly depends on liquid-liquid phase separation (LLPS) of Rubisco, a CO2 fixing enzyme. The current research on the structure, assembly and application of Chlamydomonas reinhardtii pyrenoids is summarized in this article, providing new ideas for improving crop photosynthetic performance and yield.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Julian Wichmann, Annibel Eggert, Liam D. H. Elbourne, Ian T. Paulsen, Kyle J. Lauersen, Olaf Kruse
Summary: In this study, the accessibility of different isoprenoid precursor pools for sesquiterpenoid production in Chlamydomonas reinhardtii was investigated. It was found that both the cytoplasm and chloroplast can be utilized for the generation of sesquiterpenoids. Targeting the fusion protein to the chloroplast and increasing gene dosage significantly improved sesquiterpene production. In addition, potential prenyl unit transporters were proposed based on bioinformatic analyses, which could enhance the capabilities of sesquiterpenoid production.
MICROBIAL CELL FACTORIES
(2022)
Article
Agricultural Engineering
Jia-Yi Lin, I-Son Ng
Summary: This study used genetic engineering to enhance the growth, lipid and lutein production of Chlamydomonas reinhardtii. CRISPRi was applied to down-regulate the phosphoenolpyruvate carboxylase (PEPC1) gene, resulting in increased carbon flux towards lipid synthesis. The engineered strain PGi showed the highest biomass, lipid and lutein production at 35°C.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Jia-Yi Lin, Sefli Sri Wahyu Effendi, I-Son Ng
Summary: This study explores the use of genetic modification to enhance CO2 capture and utilization in microalgae for increased biomass and product production. The results show that genetically modified C. reinhardtii can achieve maximum biomass, lutein, and lipid production under autotrophic conditions in a photo-bioreactor with 5% CO2, with improved CO2 assimilation rates.
BIORESOURCE TECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Yimin Chen
Summary: Photobiological hydrogen production through algae is a promising way to obtain green hydrogen energy, but its sustainability and cost hinder large-scale commercial production. This study presents a blueprint for sustained bioH(2) production and evaluates its economic feasibility locally. By comparing different methods and conducting geographic analysis, the potential of algal bioH(2) production is demonstrated.
ENERGY & ENVIRONMENTAL SCIENCE
(2022)
Article
Plant Sciences
Inmaculada Couso, Amanda L. Smythers, Megan M. Ford, James G. Umen, Jose L. Crespo, Leslie M. Hicks
Summary: This study reveals the synergistic role of inositol pyrophosphates and TOR signaling in coordinating cell growth and lipid metabolism in green algae. The inhibitory effect of rapamycin on TOR signaling is more significant in the vip1-1 mutant compared to wild-type, highlighting the independent yet coordinated interaction between InsPs and TOR. Additionally, phosphorylation patterns of photosystem proteins are affected by light stress and other stresses, indicating a novel role for inositol pyrophosphates and TOR signaling in response to environmental cues.
Article
Plant Sciences
Yu-Ting Li, Ying Li, Jian-Min Song, Qian-Huan Guo, Cheng Yang, Wen-Jing Zhao, Jun-Yan Wang, Jiao Luo, Yan-Ni Xu, Qiang Zhang, Xin-Yu Ding, Ying Liang, Yue-Nan Li, Qiu-Ling Feng, Peng Liu, Hui-Yuan Gao, Geng Li, Shi-Jie Zhao, Zi-Shan Zhang
Summary: Research in the past 60 years has shown that wheat breeding has optimized photosynthetic performance under high light conditions, despite a decrease in light levels on flag leaves over time. The stability of photosynthetic rates and resistance of the photosynthetic apparatus to high light, even with decreasing light intensity, may have contributed to increased wheat yields.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Environmental Sciences
Lisa Zaouk, Anthony Masse, Patrick Bourseau, Samir Taha, Murielle Rabiller-Baudry, Sebastien Jubeau, Benoit Teychene, Jeremy Pruvost, Pascal Jaouen
ENVIRONMENTAL TECHNOLOGY
(2020)
Article
Engineering, Mechanical
H. Ouldrebai, E. K. Si-Ahmed, M. Hammoudi, J. Legrand, Y. Salhi, J. Pruvost
EXPERIMENTAL TECHNIQUES
(2019)
Article
Biotechnology & Applied Microbiology
Antoinette Kazbar, H. Marec, H. Takache, A. Ismail, J. Pruvost
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
R. Nghiem Xuan, I Safitri, J. L. Mouget, J. Pruvost, V Turpin, P. Jaouen
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Energy & Fuels
Emeka G. Nwoba, David A. Parlevliet, Damian W. Laird, Kamal Alameh, Julien Louveau, Jeremy Pruvost, Navid R. Moheimani
Article
Biotechnology & Applied Microbiology
Jack Hoeniges, Razmig Kandilian, Chuanxin Zhang, Jeremy Pruvost, Jack Legrand, Dominique Grizeau, Laurent Pilon
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Biotechnology & Applied Microbiology
I Gifuni, L. Lavenant, J. Pruvost, A. Masse
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Energy & Fuels
Vladimir Heredia, Olivier Goncalves, Luc Marchal, Jeremy Pruvost
Summary: The biological responses of two algae species in nitrogen limitation and day-night cycles for energy-rich compound accumulation were investigated, with Parachlorella kessleri showing good potential for biodiesel and bioethanol production, while Nannochloropsis gaditana was found to be more efficient for biodiesel production.
Article
Chemistry, Medicinal
Antoine Decamp, Orane Michelo, Christelle Rabbat, Celine Laroche, Dominique Grizeau, Jeremy Pruvost, Olivier Goncalves
Summary: A new methodological approach relying on enzyme specificity was developed to estimate the composition of bioactive polysaccharides produced by microalgae directly in algal cultures. This method allows for routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors, with high resolution and without the constraints associated with traditional chromatographic methods.
Article
Green & Sustainable Science & Technology
Camilo Duran Quintero, Anne Ventura, Olivier Lepine, Jeremy Pruvost
Summary: This study investigates the environmental impacts of Spirulina production through a modeled production scenario combined with Morris sensitivity analysis. It finds a large variability in global warming potential depending on key parameters such as thermal regulation, setpoint temperature, and production period. By avoiding thermal regulation impacts and increasing biomass productivity, it is possible to reduce the global warming potential per unit of biomass. Expanding the cultivation period under suitable solar conditions can also contribute to higher biomass growth.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Multidisciplinary
Hareb Al Jabri, Aumaya Taleb, Raphaelle Touchard, Imen Saadaoui, Vincent Goetz, Jeremy Pruvost
Summary: Temperature and light are crucial factors for microalgae production, affecting their growth kinetics, physiological properties, and adaptation mechanisms. In the Qatar desert, there are wide variations in temperature and light intensity, making it essential to study their effects on microalgae for large-scale outdoor production systems. Studies show that dynamic light and temperature have interactive effects on biomass productivity, with microalgae showing a high level of adaptation to fluctuations in light and temperature stress.
APPLIED SCIENCES-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Vladimir Heredia, Jeremy Pruvost, Olivier Goncalves, Delphine Drouin, Luc Marchal
Summary: The aim of this study is to track and optimize the extraction of lipids from Nannochloropsis gaditana in wet extraction operations. Disruption efficiency of microalgal suspensions varied depending on their physiological states, with different methods used to recover the fatty acids released. The interaction between biomass concentration and solvent inlet rate was found to have the greatest influence on lipid recovery.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Vladimir Heredia, Luc Marchal, Olivier Goncalves, Jeremy Pruvost
Summary: This study investigates the optimization of TAG production in microalgae by continuous nitrogen-limited culture, focusing on mechanical resistance of strains and optimal harvesting time. The study compared continuous nitrogen addition to pulsed addition, with continuous addition resulting in the highest released TAG.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Rebiha Adjout, Jean-luc Mouget, Jeremy Pruvost, Imene Chentir, Celine Loiseau, Mohammed Bey Baba Hamed
Summary: This study investigated the growth and biochemical composition of H. ostrearia under different temperatures, irradiances, and pH values. The results showed that the production of specific metabolites can be controlled under specific conditions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jeremy Pruvost, Benjamin Le Gouic, Jean-Francois Cornet
Summary: This article introduces and validates a model that relates the effects of parameters on CO2 biofixation rates and CO2 use efficiency. It also discusses the different gas-liquid mass transfer performances of mechanically stirred and airlift systems. The article concludes that covered raceways have a higher CO2 use efficiency, while airlift systems have a higher CO2 supply but lower CO2 use efficiency.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
