Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Agricultural Engineering
Hongxin Fu, Huihui Zhang, Xiaolong Guo, Lu Yang, Jufang Wang
Summary: In this study, the carbon catabolite repression (CCR) in Clostridium tyrobutyricum was successfully eliminated by inactivating hprK and xylR genes, leading to increased butyric acid production and excellent fermentation performance using different lignocellulosic biomass.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Tingting Liu, Matthew Jay Malkmes, Liying Zhu, He Huang, Ling Jiang
Summary: A novel process combining Metal-Organic Frameworks (MOFs) with simultaneous saccharification and fermentation for butyric acid production was developed, resulting in increased productivity, stability, and efficiency of butyric acid production from rice straw. This innovative approach demonstrates the potential for utilizing abundant low-cost feedstocks for sustainable chemical production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hongxin Fu, Meng Lin, I-Ching Tang, Jufang Wang, Shang-Tian Yang
Summary: In this study, benzyl viologen (BV) was utilized to enhance butyric acid production by Clostridium tyrobutyricum from glucose, while eliminating acetic acid accumulation. The addition of BV increased butyric acid yield and productivity, inhibited acetate production, and promoted glucose and acetate co-utilization for butyric acid production. Manipulating metabolic flux and redox balance through BV and acetate addition provided a simple approach for efficient butyric acid production.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Yukai Suo, Wenyi Li, Liqiong Wan, Linshuang Luo, Shuang Liu, Shiwen Qin, Jufang Wang
Summary: Lignocellulosic biomass is a rich and renewable feedstock for biobased butyric acid production. However, the presence of furan derivatives from the pretreatment of lignocellulose severely inhibits the growth of the best strain for producing butyric acid, Clostridium tyrobutyricum. This study investigated the tolerance mechanism of C. tyrobutyricum to furan derivatives and found that upregulation of genes related to membrane transport, heat shock proteins, and transcriptional regulation occurs under furan derivative stress. Additionally, it was discovered that C. tyrobutyricum is unable to rapidly convert furan derivatives to low-toxicity alcohols, leading to its low tolerance. These findings provide insights into the development of furan derivative-tolerant strains for enhanced bioconversion of lignocellulosic biomass to butyric acid.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yueying Lin, Wanjing Sun, Geng Wang, Haohan Chen, Xun Pei, Yuyue Jin, Shang-Tian Yang, Minqi Wang
Summary: Rice husk hydrolysate can be effectively utilized for the bioproduction of butyrate with immobilized-cell fermentation.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Linqi Zhao, Gaoya Sun, Jing Jiang, Li Chen, Jin Huang
Summary: Microbubbles coupling fermentation technology improved oxygen transfer to aerobic microorganisms, but its effect on anaerobic Clostridium tyrobutyricum had not been reported. Evaluation showed that using microbubbles coupling fibrous-bed bioreactor (MBFBB) led to shorter fermentation time and higher butyrate productivity compared to conventional fibrous-bed bioreactor (FBB). The long-term stability and reliability for butyrate production using brewer's spent grain (BSG) were achieved in ten cycle process.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ryan J. Stoklosa, Carrington Moore, Renee J. Latona, Nhuan P. Nghiem
Summary: This study investigated cultivating Clostridium tyrobutyricum to produce butyric acid using pretreated sweet sorghum bagasse (SSB) hydrolysate. Results showed that up to 11.4 g/L of butyric acid with a selectivity of 87% could be produced, with similar outputs achieved in bioreactor fermentation with pH control. Further improvements in butyric acid titer and yield can be achieved through optimizing nutrient supplementation and fed-batch fermentation processing of pretreated SSB hydrolysate.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Li Cao, Yue Gao, Xue-Zhen Wang, Guang-Yuan Shu, Ya-Nan Hu, Zong-Ping Xie, Wei Cui, Xiao-Peng Guo, Xiang Zhou
Summary: Clostridium tyrobutyricum is a fermentation strain used to produce butyric acid, which can be catalyzed to produce n-butanol, a promising biofuel. Butyric acid has various uses and has been approved as a food flavoring by the EU. Heavy-ion irradiation can enhance butyric acid production, and mathematical models have been developed to predict fermentation outcomes using mutant strains of C. tyrobutyricum.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Bing Wang, Xiang Zhou, Wei Liu, Mei-Han Liu, Dan Mo, Qing-Feng Wu, Ya-Juan Wang, Miao-Miao Zhang, Lei Chen, Shan Yuan, Bo Zhou, Xin Li, Dong Lu
Summary: This study developed a method to pretreat refinery final molasses using acid-base treatment and dynamic ion-exchange membrane technology, making it a suitable substrate for C. tyrobutyricum fermentation to produce butyric acid. The sequential filtration with dynamic ion-exchange membranes effectively removed impurities and harmful substances while retaining fermentable sugars. This innovative approach provides industrial feasibility and economic competitiveness for the utilization of refinery final molasses by C. tyrobutyricum.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nasib Qureshi, Siqing Liu, Badal C. Saha
Summary: This study evaluated the ability of Clostridium tyrobutyricum NRRL 67062 to produce butyric acid during glucose fermentation. The findings showed that high concentrations of glucose and butyric acid strongly inhibited cell growth and fermentation. The strain exhibited a butyric acid tolerance of 58 g L-1, and the use of corn steep liquor as a nutrient solution improved both cell growth and acid production.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Edouard Munier, Helene Licandro, Eric Beuvier, Remy Cachon
Summary: This study aims to understand the production of butyrate, hydrogen, and CO2 from the oxidation of lactate with acetate in Clostridium tyrobutyricum and proposes an alternative carbon source to glucose. By analyzing the genome, genes involved in butyrate formation and lactate oxidation were identified, and a metabolic scheme was constructed and confirmed. This study improves our understanding of lactate oxidation metabolic pathways and paves the way for the production of butyrate and hydrogen with C. tyrobutyricum.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Camila A. B. Silva Rabelo, Alfonso M. Vidal, Rocio Casanova-Gonzalez, Miguel Romero-Garcia, Maria Bernadete A. Varesche, Inmaculada Romero, Eulogio Castro
Summary: This study investigates the use of vine shoots as a renewable and widely available feedstock for hydrogen production. The results showed that physical, chemical, and biological pre-treatments can enhance the hydrolysis and fermentation of vine shoots into hydrogen. Laccase pre-treatment was found to be the most effective, leading to increased production of soluble sugars and hydrogen. Additionally, the addition of cellulase during hydrolysis further improved hydrogen production. These findings have practical implications in the field of biomass energy.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agronomy
Bing Wang, Xiang Zhou, Jun-Le Ren, Miao-Miao Zhang, Qing-Feng Wu, Shan Yuan, Wei Liu, Dong Lu
Summary: In this study, molasses was pretreated to meet the requirements of Clostridium tyrobutyricum fermentation. Through microbial adaptive laboratory evolution, C. tyrobutyricum was able to convert fructose and glucose in the treated molasses into butyric acid.
Article
Biotechnology & Applied Microbiology
Karlo Guerrero, Roberto Gallardo, Ernesto Gonzalez, Fabian Veliz, Raul Conejeros, Juan Carlos Gentina, German Aroca
Summary: By adding butyrate and neutral red to the culture medium, and using electro-fermentation with an external electron source, the production of butanol can be increased. However, the presence of butyrate and the absence of neutral red in electro-fermentation are detrimental to the microorganism.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)