Editorial Material
Orthopedics
Mario Hevesi, Jorge Chahla
Summary: Hip cartilage defects commonly occur in the anterosuperior acetabulum and central femoral head. Chondrolabral delamination can be treated with repair, but the treatment of articular defects varies in outcomes. Hip chondral lesions consistently predict arthroplasty after arthroscopy. Microfracture treatment has decreased in popularity due to similar outcomes to debridement alone, longer operation time, and potential complications. Debridement is recommended for small-to-moderate full-thickness chondral defects, while novel treatments are needed for grade III to IV defects. Biologically enhanced microfracture with platelet-rich plasma, micronized allograft extracellular cartilage matrix, and fibrin glue is one possible approach.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Lourdes Alcaide-Ruggiero, Veronica Molina-Hernandez, Juan Morgaz, J. Andres Fernandez-Sarmiento, Maria M. Granados, Rocio Navarrete-Calvo, Jose Perez, Setefilla Quiros-Carmona, Jose M. Carrillo, Ramon Cugat, Juan M. Dominguez
Summary: This study evaluates the efficacy of an autologous-based particulated autograft cartilage and platelet-rich plasma treatment for repairing cartilage defects. The results show that PACI + PRP treatment promotes the repair process, resulting in mature hyaline cartilage with enhanced structural and mechanical qualities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mauro Vasella, Kevin Arnke, Dalia Dranseikiene, Elia Guzzi, Francesca Melega, Gregory Reid, Holger Jan Klein, Riccardo Schweizer, Mark W. Tibbitt, Bong-Sung Kim
Summary: In this study, the feasibility of using methacrylated gelatin (GelMA) as a scaffold for mechanically processed stromal vascular fraction (mSVF) was investigated. The results showed that GelMA maintained cell viability and proliferation, and released growth factors, which had a promoting effect on both mSVF and dermal fibroblasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Jingchuan Zheng, Zhijun Zhao, Yongdong Yang, Shuo Wang, Yonggang Zhao, Yang Xiong, Shuhui Yang, Zhiye Qiu, Tianxi Song, Chunyang Zhang, Xiumei Wang
Summary: In this study, a biphasic mineralized collagen-based bone scaffold was successfully used to repair large-sized cranial bone defects in sheep. The scaffold showed good interfacial bond between the porous and compact phases, providing appropriate mechanical protection and structural integrity.
REGENERATIVE BIOMATERIALS
(2022)
Article
Orthopedics
Jorge Chahla, Brady T. Williams, Adam B. Yanke, Jack Farr
Summary: Focal chondral defects (FCDs) of the knee are a common and severe condition that can cause pain and dysfunction in young patients with high activity demands. Recent years have seen significant advancements in the understanding of FCDs etiology and surgical management, leading to an increase in surgical procedures. Treatment options range from arthroscopic debridement to cell-based techniques, and a comprehensive understanding of management is crucial for treating surgeons.
JOURNAL OF KNEE SURGERY
(2023)
Article
Engineering, Biomedical
Vishal Joseph Thomas, Nathan Foster Buchweitz, Jay John Baek, Yongren Wu, Jeremy John Mercuri
Summary: Focal chondral defects (FCDs) significantly affect patients' quality of life and impose economic costs on society. The study aimed to develop a scaffold for cartilage repair that contains both sulfated glycosaminoglycans (sGAGs) and collagen type II. The study evaluated different protocols for decellularizing bovine nucleus pulposus (bNP) and characterized the resulting scaffold's biochemistry, biomechanics, and cytocompatibility. The optimized decell protocol involved pre-crosslinking the tissue and resulted in a scaffold with preserved viscoelastic properties and decreased equilibrium properties compared to non-decelled fresh bNP tissue. The scaffold was also found to be cytocompatible with bone marrow stem cells (BMSCs), although it did not allow cellular infiltration.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Materials Science, Multidisciplinary
Zhiming Zhang, Zijie Rong, Guofeng Wu, Yihan Wang, Zhiwen Tan, Juan Zheng, Yanglei Jin, Zhihao Liang, Chun Liu, Jiasong Guo, Lixin Zhu
Summary: The study introduced a controlled-oxygen-generating composite scaffold to enhance seed cell survival and improve bone defect reconstruction.
APPLIED MATERIALS TODAY
(2021)
Article
Materials Science, Multidisciplinary
Bo Chen, Zhengjie Lin, Qimanguli Saiding, Yongcan Huang, Yi Sun, Xinyun Zhai, Ziyu Ning, Hai Liang, Wei Qiao, Bingsheng Yu, W. K. Kelvin Yeung, Jie Shen
Summary: The use of three-dimensional (3D) printed biocomposite scaffolds, reinforced with magnesium oxide (MgO), has shown promising results in the treatment of critical-sized bone defects (CSD). The incorporation of magnesium ions (Mg2+) in the scaffold enhances mechanical performance and cytocompatibility, leading to improved bone regeneration and repair.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Elena I. Pappa, Mariana S. Barbagianni, Stefanos G. Georgiou, Labrini V. Athanasiou, Dimitra Psalla, Dionysios Vekios, Eleni I. Katsarou, Natalia G. C. Vasileiou, Pagona G. Gouletsou, Apostolos D. Galatos, Nikitas N. Prassinos, Dimitris A. Gougoulis, Marianna Angelidou, Vicky Tsioli, George C. Fthenakis, Aikaterini I. Sideri
Summary: This study evaluated the efficacy of using autologous stromal vascular fraction from adipose tissue in enhancing bone healing. The findings suggest that the use of stromal vascular fraction can accelerate post-operative rehabilitation and significantly enhance bone formation, with osteogenic potential comparable to autologous bone graft.
Article
Medicine, General & Internal
Jensen G. Kolaczko, Lucas Haase, Matthew Kaufman, Jacob Calcei, Michael R. Karns
Summary: In this retrospective study, the presence of effusion was found to be the only parameter correlated with occult osteochondral injuries, while other parameters showed no association.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2022)
Article
Orthopedics
Yoshikazu Sumida, Kaori Nakamura, Sven Feil, Maja Siebold, Joachim Kirsch, Rainer Siebold
Summary: Arthroscopic second-look evaluation showed that all-arthroscopic ACI for patellar cartilage defects resulted in high-quality cartilage regeneration, with most lesions classified as normal or nearly normal. Overall, the majority of defects were repaired effectively, indicating the reproducibility and reliability of the procedure.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2022)
Article
Materials Science, Textiles
Amir Nahanmoghadam, Maryam Asemani, Vahabodin Goodarzi, Somayeh Ebrahimi-Barough
Summary: A composite scaffold incorporating PHBV and PCL polymers with 5% HA nanoparticles was successfully fabricated for bone critical size defect regeneration. The scaffold showed enhanced bone regeneration and decreased fibrous connective tissue formation compared to other groups.
FIBERS AND POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Elia Bari, Franca Scocozza, Sara Perteghella, Lorena Segale, Marzio Sorlini, Ferdinando Auricchio, Michele Conti, Maria Luisa Torre
Summary: The study demonstrated that combined printing of Lyosecretome in scaffolds can enhance osteogenic effects, promote MSC differentiation towards bone cells in osteogenic medium, and achieve better bone formation at 14 and 28 days.
Article
Chemistry, Multidisciplinary
Abdulsamet Emet, Erdi Ozdemir, Duygu Uckan Cetinkaya, Emine Kilic, Ramin Hashemihesar, Ali Celalettin Sinan Yuruker, Egemen Turhan
Summary: The study found that the combination of decellularized omentum scaffold with PRP and mesenchymal stem cells can promote the healing of critical-sized bone defects. The use of decellularized omentum scaffold maintains the vitality of new cells and enhances new bone formation, leading to better radiological and histological healing compared to the control group.
APPLIED SCIENCES-BASEL
(2021)
Article
Nanoscience & Nanotechnology
Keigo Shibahara, Koichiro Hayashi, Yasuharu Nakashima, Kunio Ishikawa
Summary: The study evaluated the ability of CAp HC scaffolds by reconstructing segmental defects in the rabbit ulnar shaft, showing that the scaffolds promoted bony callus formation at 4 weeks post-surgery and connected to the host bone at 12 weeks post-surgery in 10 and 15 mm defects. The results indicate that CAp HC scaffolds have good potential value for the reconstruction of critical-sized defects.
ACS APPLIED BIO MATERIALS
(2021)
Article
Oncology
Maria Villamonte, Fernando Burdio, Eva Pueyo, Ana Andaluz, Xavier Moll, Enrique Berjano, Aleksander Radosevic, Luis Grande, Miguel Pera, Benedetto Ielpo, Patricia Sanchez-Velazquez
Summary: The study aimed to compare the impact of an additional application of RF energy on the top of the resection surface on local recurrence in liver surgery. The results showed that additional margin coagulation (AMC) was associated with lower local recurrence, and the use of RF applicator was significantly related to reduced local recurrence. The Control group had smaller coagulation widths compared to the AMC group.
Article
Biology
Noelia Munoz-Dominguez, Irene Carreras-Sanchez, Alba Lopez-Fernandez, Joaquim Vives
Summary: This study presents a simple, efficient and reproducible method for cryopreservation of unprocessed UC tissue fragments, ensuring the quality attributes of the cells, and applicable for large-scale derivation and storage of UC-MSC.
Article
Cell & Tissue Engineering
Jesus Fernandez-Sojo, Joaquim Delgadillo, Joaquim Vives, Luciano Rodriguez, Ana Mendoza, Carmen Azqueta, Ana Garcia-Buendia, Elena Valdivia, Lluis Martorell, Nuria Rubio-Lopez, Monica Linares, Sofia Alonso, Agueda Ancochea, Enric Garcia-Rey, Nadia Garcia-Munoz, Laura Medina, Sergio Querol
Summary: This study describes and analyzes whether a hub-and-spoke organizational model can efficiently provide access to chimeric antigen receptor (CAR) T-cell therapy within a network of academic hospitals and address the growing demands of this complex and specialized activity. The results show that the model is efficient in incorporating CAR T-cell therapy into a public health hospital network, based on a retrospective evaluation of the Catalan Blood and Tissue Bank network.
Article
Oncology
Xavier Moll, Dolors Fondevila, Felix Garcia-Arnas, Fernando Burdio, Macarena Trujillo, Ramiro M. Irastorza, Enrique Berjano, Anna Andaluz
Summary: This study compared the coagulation zones created by two radiofrequency-based hemostatic devices and found that both external irrigation and internal cooling effectively prevented tissue sticking and created similar coagulation zones from a histological perspective. Saline-linked bipolar electrodes tended to create shallower coagulations than internally cooled monopolar electrodes.
INTERNATIONAL JOURNAL OF HYPERTHERMIA
(2022)
Article
Immunology
Juan Flores-Medina, Ana Lopez-Navas, Laura Martinez-Alarcon, Guillermo Ramis, Xavier Moll, Javier Gomez-Laguna, Pedro Gutierrez, Antonio Rios
Summary: This study analyzed the influence of religious beliefs on the attitude toward xenotransplantation in veterinary students. The results showed that individuals who practice Catholicism and those who consider themselves agnostic/atheist are more accepting of organ procurement for transplantation.
TRANSPLANTATION PROCEEDINGS
(2022)
Article
Multidisciplinary Sciences
Cristian Melgarejo, Carles Planas, Alex Cobos, Claudia Arrieta-Villegas, Iker A. Sevilla, Javier Bezos, Xavier Moll, Yvonne Espada, Joseba M. Garrido, Mariano Domingo, Enric Vidal, Bernat Perez de Val
Summary: This study assessed the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats challenged with the same strain of M. caprae. The findings showed that vaccinated goats had reduced lung lesions and bacterial load compared to unvaccinated controls, indicating enhanced protection against M. caprae challenge.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Lluis Martorell, Alba Lopez-Fernandez, Andrea Garcia-Lizarribar, Roger Sabata, Patricia Galvez-Martin, Josep Samitier, Joaquim Vives
Summary: Three dimensional (3D) bioprinting is an emerging technology with vast therapeutic potential in regenerative medicine. The success of 3D bioprinting relies on defining a bioprintable zone specific to each combination of hydrogels and scaffolds, which matches the mechanical and biological characteristics of the target tissue. This study investigates the advantages of natural hydrogel-based bioinks in preserving critical quality attributes (CQA) in bone tissue regeneration applications, particularly focusing on cell viability and osteogenic potential of multipotent mesenchymal stromal cells (MSCs) in vitro. The use of hyaluronic acid, fibrin, and gelatin as bioinks allows for optimal recovery of viable cells while maintaining MSC's proliferation capacity and osteogenic potency.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Immunology
Silvia Torrents, Andres Escudero del Moral, Margarita Codinach, Luciano Rodriguez, Sergi Querol, Joaquim Vives
Summary: Multipotent mesenchymal stromal cells (MSC) have the potential to modulate an imbalanced immune system, and a reliable immunopotency assay has been established using MSC-WJ co-culture and cryopreserved PBMC. This assay provides a standardized method for assessing the immunomodulatory potency of MSC and contributes to the harmonization of biological reagents usage.
IMMUNOLOGIC RESEARCH
(2023)
Article
Oncology
Xavier Moll, Dolors Fondevila, Felix Garcia-Arnas, Juan J. Perez, Benedetto Ielpo, Patricia Sanchez-Velazquez, Luis Grande, Sofia Jaume, Aleksandar Radosevic, Luis Barranco, Enrique Berjano, Fernando Burdio, Anna Andaluz
Summary: Pre-clinical studies suggest that TAMPD is more recommendable than glue for reducing POPF. This study aims to analyze the changes in the pancreas of patients after TAMPD and correlate the clinical findings with an animal model study.
INTERNATIONAL JOURNAL OF HYPERTHERMIA
(2023)
Article
Multidisciplinary Sciences
Amirhossein Sarreshtehdari, Fernando Burdio, Borja Lopez-Alonso, Oscar Lucia, Jose Miguel Burdio, Maria Villamonte, A. Andaluz, F. Garcia-Arnas, E. Berjano, Xavier Moll
Summary: The study investigated the effects of injecting glucose solution 5% on the impedance of tissue and its potential in focusing irreversible electroporation on liver tumors. The results showed a significant impedance alteration and no side effects on healthy liver tissue after glucose solution infusion.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Joaquim Vives, Fermin Sanchez-Guijo, Massimiliano Gnecchi, Jaap Jan Zwaginga
Summary: The development and production of cell gene and tissue therapies require a specialized workforce. Currently, there is a shortage of skilled workers in the industry and academia. Traditional educational programs are not suitable for training professionals ready to enter the fast-growing CGT field. This paper discusses the reasons for the growth acceleration in the CGT field, predicts future workforce needs, and evaluates potential solutions.
Article
Microbiology
Cristian Melgarejo, Alex Cobos, Carles Planas, Jaume Fondevila, Maite Martin, Zoraida Cervera, Guillermo Cantero, Xavier Moll, Yvonne Espada, Mariano Domingo, Enric Vidal, Bernat Perez de Val
Summary: This study assessed a new experimental model of tuberculosis (TB) in goats using intranasal nebulization of Mycobacterium caprae and compared it with endobronchial infection route. The results showed that the intranasal challenged group had slower progression of infection, lower severity and dissemination of lung lesions, and higher involvement of nasopharyngeal cavity in comparison to the endobronchial route. On the other hand, the endobronchial route accelerated the pulmonary TB lesion progression. Therefore, refinement of inoculation routes is needed for faithfully reproducing the natural TB infection when evaluating new vaccines or treatments.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Raquel Cabrera-Perez, Alexis Rafols-Mitjans, Angela Roig-Molina, Silvia Beltramone, Joaquim Vives, Laura Batlle-Morera
Summary: Our study investigated the osteogenic commitment of hard-to-handle MSC,WJ in different bone microenvironments. Co-culture experiments showed that MSC,WJ could efficiently differentiate into bone cells in the presence of osteogenic stimuli from MSC,BM. In vivo experiments demonstrated successful bone formation in the medullar cavity of tibias in mice treated with MSC,WJ. The presence of human cells in various organs indicated the potential systemic effects of engineered MSC,WJ. Overall, our findings support the use of off-the-shelf allogeneic MSC,WJ products in bone repair and regeneration.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Veterinary Sciences
Mauricio Tobon Restrepo, Rosa Novellas, Adria Aguilar, Xavier Moll, Yvonne Espada
Summary: This study revealed the features of lymph nodes in the abdomen, pelvis, and hindlimb of cats using CT and US, with CT performing better in identifying and measuring lymph nodes compared to US and anatomical studies. The height of lymph nodes showed the main statistical differences among different imaging techniques.
ACTA VETERINARIA SCANDINAVICA
(2022)
Meeting Abstract
Cell & Tissue Engineering
Joaquim Vives
TISSUE ENGINEERING PART A
(2022)