期刊
BIOTECHNOLOGY LETTERS
卷 37, 期 2, 页码 333-341出版社
SPRINGER
DOI: 10.1007/s10529-014-1691-2
关键词
Aspergillus oryzae; Quantitative real-time PCR; Repression under secretion stress; Taka-amylase A gene promoter; Truncation and deletion analysis
资金
- National Science and Technology Foundation in China [2011AA100905]
- Guangdong Provincial Department of Science and Technology Research Project [2012 B010900028]
- National Natural Science Foundation of China [31100026]
The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, which was depressed under secreted protein stress. We conducted a truncation and deletion analysis of the amyB promoter to identify cis-elements required for RESS. A nucleotide sequence (positions -378 to -291) without any binding sites for the transcriptional activator AmyR, which is involved in amylolytic gene expression, was required for RESS. The octamer sequence TCACGGGC (positions -307 to -300) constituted the core sequence of the upstream activating element essential for amyB down-regulation under secretion stress. Both the inactivation of AmyR and RESS contributed to the down-regulation of amyB expression under ER stress.
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