Article
Biotechnology & Applied Microbiology
Bo Liang, Xinping Zhang, Chenfei Meng, Lu Wang, Jianming Yang
Summary: This study improved the transport performance of the MatPQM importer by obtaining MatP variants through directed evolution and a novel enzyme-inhibition-based high throughput screening approach. The best mutant, E103G/S194G/Y218H/L235P/N272S, showed twofold increased transport efficiency compared to the wild-type. Improved binding affinity of the mutant to the ligand was found to be the reason for the enhanced uptake activity of MatPQM. The engineered strain produced 20.08 g/L 3-HP with a yield of 0.87 mol/mol malonate, demonstrating the broad applications of this transporter mutant in microbial biosynthesis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hirotaka Kudo, Sho Ono, Kenta Abe, Mami Matsuda, Tomohisa Hasunuma, Tomoyasu Nishizawa, Munehiko Asayama, Hirofumi Nishihara, Shigeru Chohnan
Summary: Enhancing the CoA biosynthetic pathway with exogenous CoaA effectively increased P(3HB) production. Supplementing the medium with pantothenate or beta-alanine facilitated the accumulation of P(3HB).
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Changfeng Miao, Dong Meng, Yuling Liu, Fang Wang, Lu Chen, Zhaosong Huang, Xiangyu Fan, Pengfei Gu, Qiang Li
Summary: This study identified a phaC(R) gene encoding PHA synthase in Rhodoligotrophos defluvii, and successfully synthesized different content and structural PHA copolymers under different carbon source conditions, achieving efficient biosynthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Fangting Wu, Ying Zhou, Wenyu Pei, Yuhan Jiang, Xiaohui Yan, Hong Wu
Summary: This study constructed an optimized pathway for PHB production using sucrose as the sole carbon source and guided gene knockout to reduce byproduct generation. The use of sucrose as a carbon source under anaerobic conditions significantly improved PHB yield and production costs.
Article
Multidisciplinary Sciences
Ryan T. Santilli, John E. Williamson, Yoshitaka Shibata, Rosalie P. Sowers, Andrew N. Fleischman, Song Tan
Summary: The Penn State Protein Ladder system produces protein molecular weight markers through plasmid expression in E. coli, allowing for easy migration, purification, and detection on SDS-PAGE gels. Additionally, polycistronic expression vectors have been designed for more efficient production and as positive controls for recombinant protein expression and purification in research laboratories.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Shu Saito, Ryu Imai, Yuki Miyahara, Mari Nakagawa, Izumi Orita, Takeharu Tsuge, Toshiaki Fukui
Summary: This study successfully introduced an artificial pathway for the biosynthesis of P(3HB-co-3HHx) into Escherichia coli, resulting in higher production and 3HHx composition by modifying genes and adding a phosphite oxidation system.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiao-Ran Jiang, Xu Yan, Lin-Ping Yu, Xin-Yi Liu, Guo-Qiang Chen
Summary: The study describes the engineering of Halomonas bluephagenesis to achieve high-level production of 3-hydroxypropionic acid (3HP) and its copolymer under open and unsterile conditions by deleting a newly identified degradation pathway and balancing redox state.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Thanawat Boontip, Rungaroon Waditee-Sirisattha, Kohsuke Honda, Suchada Chanprateep Napathorn
Summary: This study aimed to increase PHB production by enhancing the expression of a PHB biosynthesis operon derived from alkaline-tolerant strain C. necator A-04. Through careful optimization, soluble phaC(A-04) protein was successfully expressed using a fusion protein with TF under a cold shock promoter, leading to high PHB productivity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ayaat M. Mahmoud, Cameron J. Nowell, Orlagh Feeney, Leonie van 't Hag, Thomas P. Davis, Kristian Kempe
Summary: An in-depth understanding of the physicochemical properties of nanocarriers is crucial for the development of novel delivery systems. In this study, well-defined hydrophobic carboxylated poly(3-hydroxypropionate)-based comb polymers were synthesized and characterized. The study demonstrated the importance of hydrophobicity in tuning the cellular interactions of carboxylated polymers for future drug carrier design.
Article
Biotechnology & Applied Microbiology
Nga Yu Poon, Anthony J. Sinskey, Kang Zhou
Summary: The threat of global plastic waste accumulation has led to the exploration of bioplastics. A proposed pathway for assimilating 1,3-propanediol (1,3-PDO) into central carbon metabolism was investigated using beta-alanine as a surrogate substrate. The study successfully engineered E. coli to assimilate beta-alanine and identified two enzymes from Vibrio natriegens that completed the metabolic pathway.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yang Li, Ying Wang, Ruxiang Wang, Xiongying Yan, Jiewen Wang, Xia Wang, Shouwen Chen, Fengwu Bai, Qiaoning He, Shihui Yang
Summary: In this study, a heterologous poly-3-hydroxybutyrate (PHB) pathway was engineered into Zymomonas mobilis, resulting in increased PHB production. By combining genetic strategies and process engineering, the researchers were able to optimize PHB content in recombinant strains and even generate a self-flocculating strain with higher PHB content. Furthermore, Z. mobilis showed potential for continuous co-production of high-titer ethanol and PHB.
Article
Biotechnology & Applied Microbiology
Joanne Hothersall, Sandie Lai, Nan Zhang, Rita E. Godfrey, Patcharawarin Ruanto, Sarah Bischoff, Colin Robinson, Tim W. Overton, Stephen J. W. Busby, Douglas F. Browning
Summary: This article describes a vector system controlled by the nitrate-responsive transcription activator protein in Escherichia coli for high-level controlled recombinant protein production. The use of nitrate allows for a simplified and cost-effective approach, as it is a cheap and abundant inorganic ion. The study demonstrates the expression of target proteins to high levels using this system and shows that inexpensive garden products can be used for controlled protein production due to the high nitrate levels present in commercial fertilizers.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Meenakshi Venkatesan, Cameron Semper, Stig Skrivergaard, Rosa Di Leo, Nathalie Mesa, Martin Kroyer Rasmussen, Jette Feveile Young, Margrethe Therkildsen, Peter J. Stogios, Alexei Savchenko
Summary: This study presents a cost-effective method for producing functionally active GFs in Escherichia coli, which can significantly reduce the cost of cell culture medium and facilitate the production of cell-cultured meat and tissue engineering.
Review
Biotechnology & Applied Microbiology
Albert Gyapong Aduhene, Hongliang Cui, Hongyi Yang, Chengwei Liu, Guangchao Sui, Changli Liu
Summary: Poly(3HP) is a novel biomaterial with biocompatibility, biodegradability, and high yield synthesis, which has been widely used in the bioplastic industry. The development of bio-polymer-based functional composites helps increase the value of raw microbial-biopolymers obtained from cheap sources.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Anindita Paul, Linjing Jia, Erica L. -W. Majumder, Chang Geun Yoo, Karthik Rajendran, Esteban Villarreal, Deepak Kumar
Summary: In this study, a mild two-stage hydrothermal pretreatment was used to convert industrial hemp fibrous waste into sugars for PHB production. The highest glucose and cellulose conversion were achieved with HWDM at 200 degrees C for 10 min. Fermentation with pH control resulted in a significant increase in PHB yield.
BIORESOURCE TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Yujin Cao, Rubing Zhang, Wei Liu, Guang Zhao, Wei Niu, Jiantao Guo, Mo Xian, Huizhou Liu
SCIENTIFIC REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Xinjun Feng, Wenjie Gao, Yifei Zhou, Zhiqiang Zhao, Xiutao Liu, Xiaojuan Han, Mo Xian, Guang Zhao
ENGINEERING IN LIFE SCIENCES
(2019)
Article
Biotechnology & Applied Microbiology
Cheng Wang, Junhua Wang, Jian Yuan, Lingyan Jiang, Xiaolong Jiang, Bin Yang, Guang Zhao, Bin Liu, Di Huang
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Review
Biotechnology & Applied Microbiology
Zhe Zhao, Mo Xian, Min Liu, Guang Zhao
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Biochemical Research Methods
Min Liu, Yingxin Fu, Wenjie Gao, Mo Xian, Guang Zhao
ACS SYNTHETIC BIOLOGY
(2020)
Article
Biochemical Research Methods
Jia Zhang, Yefei Wang, Baihui Chai, Jichao Wang, Lulu Li, Min Liu, Guang Zhao, Lishan Yao, Xiaolian Gao, Yifeng Yin, Jian Xu
ACS SYNTHETIC BIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Yifei Zhou, Yamei Ding, Wenjie Gao, Jichao Wang, Xiutao Liu, Mo Xian, Xinjun Feng, Guang Zhao
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Multidisciplinary Sciences
Ying Xu, Zhe Zhao, Wenhua Tong, Yamei Ding, Bin Liu, Yixin Shi, Jichao Wang, Shenmei Sun, Min Liu, Yuhui Wang, Qingsheng Qi, Mo Xian, Guang Zhao
NATURE COMMUNICATIONS
(2020)
Article
Biotechnology & Applied Microbiology
Shenmei Sun, Yamei Ding, Min Liu, Mo Xian, Guang Zhao
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jichao Wang, Xinyue Sui, Yamei Ding, Yingxin Fu, Xinjun Feng, Min Liu, Youming Zhang, Mo Xian, Guang Zhao
Summary: The study presents a genome-editing method based on a Rock-Paper-Scissors strategy, allowing for efficient genetic modifications with reduced cultivation times. The method has been successfully applied in Escherichia coli and Klebsiella pneumoniae, demonstrating its ability to prevent spontaneous genome mutations.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Min Liu, Likun Guo, Yingxin Fu, Meitong Huo, Qingsheng Qi, Guang Zhao
Summary: Protein acetylation has potential in metabolic regulation, but the regulatory mechanisms are not fully understood.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biotechnology & Applied Microbiology
Min Liu, Meitong Huo, Changshui Liu, Likun Guo, Yamei Ding, Qingjun Ma, Qingsheng Qi, Mo Xian, Guang Zhao
Summary: Protein lysine acetylation plays an important role in metabolic regulation, and can be used to control lactate synthesis, improve production and conversion efficiency in microbial synthesis, and provide insights into the treatment of lactate-related diseases.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Zhe Zhao, Ying Xu, Bo Jiang, Qingsheng Qi, Ya-Jie Tang, Mo Xian, Jichao Wang, Guang Zhao
Summary: The CpxRA system senses environmental stresses and regulates gene transcription for adaptation. This study identified over 10,000 putative CpxR binding sites and confirmed experimentally that 73 candidate genes are regulated by CpxR. These findings greatly expand our understanding of the CpxRA-dependent stress response network in Escherichia coli.
Article
Multidisciplinary Sciences
Likun Guo, Min Liu, Yujia Bi, Qingsheng Qi, Mo Xian, Guang Zhao
Summary: In this study, a synthetic carbon conserving pathway named SCTPK was constructed and optimized to improve the carbon atom economy in microbial cell factories. The pathway utilizes a generalist phosphoketolase Xfspk to convert glucose into stoichiometric amounts of acetylphosphate (AcP), and genetic circuits responding to AcP were created. Together with SCTPK, they form a gene-metabolic oscillator that autonomously regulates the production of valuable chemicals in response to intracellular AcP level.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Xiutao Liu, Xinjun Feng, Yamei Ding, Wenjie Gao, Mo Xian, Jichao Wang, Guang Zhao
METABOLIC ENGINEERING
(2020)