期刊
BIOTECHNOLOGY LETTERS
卷 36, 期 7, 页码 1515-1522出版社
SPRINGER
DOI: 10.1007/s10529-014-1543-0
关键词
beta-Carotene; Escherichia coli; Isoprenoid biosynthesis; Lycopene; NADPH; Ribosome-binding sites
资金
- Tianjin Key Technology R&D program of Tianjin Municipal Science and Technology Commission [12ZCZDSY1 4700]
- National Basic Research Program of China [2011CBA0 0800]
- National High Technology Research and Development Program of China [2012AA02A704]
- Hundred Talent Program of the Chinese Academy of Sciences
Escherichia coli strain CAR001 that produces beta-carotene was genetically engineered to produce lycopene by deleting genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene beta-cyclase (crtY) from the crtEXYIB operon. The resulting strain, LYC001, produced 10.5 mg lycopene/l (6.5 mg/g dry cell weight, DCW). Modulating expression of genes encoding alpha-ketoglutarate dehydrogenase, succinate dehydrogenase and transaldolase B within central metabolic modules increased NADPH and ATP supplies, leading to a 76 % increase of lycopene yield. Ribosome binding site libraries were further used to modulate expression of genes encoding 1-deoxy-d-xylulose-5-phosphate synthase (dxs) and isopentenyl diphosphate isomerase (idi) and the crt gene operon, which improved the lycopene yield by 32 %. The optimal strain LYC010 produced 3.52 g lycopene/l (50.6 mg/g DCW) in fed-batch fermentation.
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