期刊
BIOTECHNOLOGY LETTERS
卷 36, 期 3, 页码 417-426出版社
SPRINGER
DOI: 10.1007/s10529-013-1377-1
关键词
Crop plants; Functional markers; Genomic selection; Next generation DNA sequencing; Plant biotechnology; plant breeding; polymorphisms
资金
- Department of Science and Technology (DST) of Government of India
The availability of complete genome sequences, along with other genomic resources for Arabidopsis, rice, pigeon pea, soybean and other crops, has revolutionized our understanding of the genetic make-up of plants. Next-generation DNA sequencing (NGS) has facilitated single nucleotide polymorphism discovery in plants. Functionally-characterized sequences can be identified and functional markers (FMs) for important traits can be developed at an ever-increasing ease. FMs are derived from sequence polymorphisms found in allelic variants of a functional gene. Linkage disequilibrium-based association mapping and homologous recombinants have been developed for identification of perfect markers for their use in crop improvement practices. Compared with many other molecular markers, FMs derived from the functionally characterized sequence genes using NGS techniques and their use provide opportunities to develop high-yielding plant genotypes resistant to various stresses at a fast pace.
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