期刊
BIOTECHNOLOGY LETTERS
卷 35, 期 9, 页码 1509-1518出版社
SPRINGER
DOI: 10.1007/s10529-013-1226-2
关键词
Populus suaveolens; Glucose-6-phosphate dehydrogenase; Oxidative pentose phosphate pathway; Cold resistance
资金
- Nation Natural Science Foundation of China [30271093]
- Foundation for Distinguished Young Talents in Higher Education of Guangdong, China [LYM10040]
- Key Laboratory of Biomass Energy of Guangdong Higher Education Institutes, South China Agricultural University, China [BOP2012-7]
A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据