Review
Biotechnology & Applied Microbiology
Helen G. Smith, Matthew J. Beech, Jozef R. Lewandowski, Gregory L. Challis, Matthew Jenner
Summary: PKS and NRPS multienzymes produce valuable metabolites, with protein subunits requiring ordered self-assembly for correct organisation of catalytic domains. DDs at the N- and C-termini of each subunit interact in complementary pairs to facilitate substrate transfer and pathway fidelity. efforts to engineer biosynthetic pathways have focused on utilizing DDs.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biology
Lateef Babatunde Salam, Oluwafemi S. Obayori, Mutiat O. Mohammed
Summary: This study used Shotgun metagenomic analysis to explore secondary metabolites in contaminated agricultural soil, focusing on polyketide synthases and non-ribosomal peptide synthetases. The majority of detected biosynthetic genes were found to belong to type I polyketides and non-ribosomal peptides, indicating a potentially rich source of natural products with unique functionalities.
Article
Microbiology
Khanh Minh Chau, Thi Thu Hao Van, Dong Van Quyen, Hung Dinh Le, Trinh Hoai Thi Phan, Ngoc Duy Thi Ngo, Trang Dieu Thi Vo, Trung Thanh Dinh, Hoa Thi Le, Huynh Hoang Nhu Khanh
Summary: This study characterized and compared 16 potential anti-Vibrio probiotics isolated from marine sponges and fish intestines, as well as an anti-Vibrio bacteriocin, revealing a higher antimicrobial spectrum in intestinal Vi+ due to the higher gene frequency of two antimicrobial compounds. Additionally, a three-step procedure was used to purify an anti-Vibrio bacteriocin produced by B. methylotrophicus NTBD1, identifying essential properties for promising feed additives. This research provides necessary information on the potential of probiotic Bacillus species with anti-Vibrio antimicrobial properties for sustainable aquaculture.
Review
Biotechnology & Applied Microbiology
Richard H. Baltz
Summary: Microbial genome mining for drug discovery has accelerated due to advancements in genome sequencing, bioinformatics, metabolomics, and synthetic biology. The process involves sequencing microbes, identifying new secondary metabolite biosynthetic gene clusters, and implementing innovative technologies for natural product discovery.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Review
Chemistry, Physical
Maria L. Adrover-Castellano, Jennifer J. Schmidt, David H. Sherman
Summary: This review explores the general cyclization mechanism of TE domain in NRPS and PKS, including oligomerization and CLCs. It also discusses other cyclization catalysts such as C-T domains, R domains, R-D domains, Dieckmann cyclases, among others. The diversity of cyclization catalysts highlighted in the studies for the production of biologically relevant, complex cyclic natural products and related compounds is summarized here.
Article
Microbiology
Jolanta Lebedeva, Gabriele Jukneviciute, Rimvyde Cepaite, Vida Vickackaite, Raminta Pranckute, Nomeda Kuisiene
Summary: The study investigated the biosynthetic potential of two Paenibacillus sp. strains isolated from the Kruber-Voronja Cave, revealing multiple biosynthetic gene clusters and potentially novel bioactive compounds.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kajal Chakraborty, Vinaya Kizhakkepatt Kizhakkekalam, Minju Joy, Rekha Devi Chakraborty
Summary: This study isolated three novel antibacterial compounds from a seaweed-associated bacterium, showing potential activities against drug-resistant pathogens, with one compound demonstrating significantly greater antagonistic activities compared to standard antibiotics. The presence of specific genes in the bacterium supports its potential as a promising source of antimicrobial compounds with pharmaceutical and biotechnological applications.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Taylor B. Cook, Tyler B. Jacobson, Maya V. Venkataraman, Heike Hofstetter, Daniel Amador-Noguez, Michael G. Thomas, Brian F. Pfleger
Summary: This article highlights the use of Pseudomonas putida as a heterologous host for producing natural products, with genetic modifications and synthetic biology tools improving production yields. By introducing biosynthetic gene clusters encoding the synthesis of bioactive compounds, engineered strains showed robust production. Various strategies, including genome editing and increasing auxiliary protein availability, were utilized to increase titers of prodigiosin and glidobactin A.
METABOLIC ENGINEERING
(2021)
Article
Microbiology
Ambika Pokhrel, Jeffrey J. Coleman
Summary: The Fusarium solani species complex (FSSC) is a complex of at least 77 phylogenetically distinct species that includes agriculturally important and clinically relevant opportunistic pathogens. The FSSC has been found to produce a diverse range of secondary metabolites, which may contribute to its ability to colonize different habitats and hosts. This study identified a large number of secondary metabolite biosynthetic gene clusters (BGCs) in the genomes of ten FSSC fungi, including several novel BGCs and those responsible for the production of known compounds.
Review
Microbiology
Khorshed Alam, Arpita Mazumder, Suranjana Sikdar, Yi-Ming Zhao, Jinfang Hao, Chaoyi Song, Yanyan Wang, Rajib Sarkar, Saiful Islam, Youming Zhang, Aiying Li
Summary: Natural products derived from Streptomyces play a crucial role in the treatment of diseases and agriculture, yet many remain unexplored. The development of novel antibiotics, agrochemicals, and anticancer medicines is imperative due to increasing resistance. Genomic-driven drug discovery strategies offer a promising future for drug development from Streptomyces.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Peng Hou, Vincent V. Nowak, Chanel J. Taylor, Mark J. Calcott, Allison Knight, Jeremy G. Owen
Summary: In this study, 322 actinomycetes were isolated and their genome sequences were obtained from New Zealand lichens. By enumerating biosynthetic gene clusters (BGCs) and comparing to reference collections, it was found that actinomycetes from New Zealand lichens have the genetic capacity to produce large numbers of natural products, which are expected to be different from those identified in previous soil-based studies. This study sheds light on the actinomycete assemblage in New Zealand lichens and highlights the potential of lichen-sourced actinobacteria for discovering new secondary metabolites.
Article
Biochemistry & Molecular Biology
Jepri Agung Priyanto, Muhammad Eka Prastya, Rika Indri Astuti, Rhesi Kristiana
Summary: This study isolated and evaluated endophytic bacteria from Archidendron pauciflorum for their antibacterial and antibiofilm activities against multidrug-resistant strains. Seven bacteria showed antibacterial activity and extracts from four selected isolates displayed antibacterial activity. The isolates belonged to the genus Bacillus and contained genes responsible for secondary metabolite synthesis.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Shan Wang, Kewen Wu, Ya-Jie Tang, Hai Deng
Summary: This article introduces the research on alpha,beta-Dehydroamino acids (dhAAs) from 2000 to 2023 and their applications in natural products. DhAAs have synthetic flexibility and can be used in chemical reactions and biological applications. Dha and Dhb residues are the most common. The formation of other dhAA residues is less understood. The article also discusses the biosynthetic pathways of some dhAA residues.
NATURAL PRODUCT REPORTS
(2023)
Article
Microbiology
Ninfa Ramirez-Duran, Rafael R. de la Haba, Blanca Vera-Gargallo, Cristina Sanchez-Porro, Scarlett Alonso-Carmona, Horacio Sandoval-Trujillo, Antonio Ventosa
Summary: In this study, a comparative genomic analysis of the actinobacterial genus Saccharomonospora was conducted, revealing a high degree of novelty and diversity of biosynthetic gene clusters (BGCs) in members of this genus. The study supports a recent speciation event between Saccharomonospora halophila and Saccharomonospora iraqiensis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Ikechukwu C. Okorafor, Mengbin Chen, Yi Tang
Summary: A set of fungal tandem polyketide synthases has been discovered to produce high titers of olivetolic acid and related derivatives, which could enable new synthetic biology strategies for accessing microbial cannabinoids.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Katerina Nikolouli, Dimitris Mossialos
ANNALS OF MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Katerina Nikolouli, Anastasia Pournou, Glenn McConnachie, George Tsiamis, Dirnitris Mossialos
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2016)
Review
Entomology
Katerina Nikolouli, Herve Colinet, David Renault, Thomas Enriquez, Laurence Mouton, Patricia Gibert, Fabiana Sassu, Carlos Caceres, Christian Stauffer, Rui Pereira, Kostas Bourtzis
JOURNAL OF PEST SCIENCE
(2018)
Article
Microbiology
Kiriaki Karakasidou, Katerina Nikolouli, Grigoris D. Amoutzias, Anastasia Pournou, Christos Manassis, George Tsiamis, Dimitris Mossialos
Article
Entomology
K. Nikolouli, F. Sassu, L. Mouton, C. Stauffer, K. Bourtzis
JOURNAL OF PEST SCIENCE
(2020)
Article
Multidisciplinary Sciences
Fabiana Sassu, Katerina Nikolouli, Rui Pereira, Marc J. B. Vreysen, Christian Stauffer, Carlos Caceres
Article
Entomology
Fabiana Sassu, Katerina Nikolouli, Silvana Caravantes, Gustavo Taret, Rui Pereira, Marc J. B. Vreysen, Christian Stauffer, Carlos Caceres
Article
Genetics & Heredity
Katerina Nikolouli, Antonios A. Augustinos, Panagiota Stathopoulou, Elias Asimakis, Anastasios Mintzas, Kostas Bourtzis, George Tsiamis
Article
Multidisciplinary Sciences
Christopher M. Ward, Roswitha A. Aumann, Mark A. Whitehead, Katerina Nikolouli, Gary Leveque, Georgia Gouvi, Elisabeth Fung, Sarah J. Reiling, Haig Djambazian, Margaret A. Hughes, Sam Whiteford, Carlos Caceres-Barrios, Thu N. M. Nguyen, Amanda Choo, Peter Crisp, Sheina B. Sim, Scott M. Geib, Frantisek Marec, Irina Haecker, Jiannis Ragoussis, Alistair C. Darby, Kostas Bourtzis, Simon W. Baxter, Marc F. Schetelig
Summary: By using classical and modern genetic approaches, the gene responsible for the white pupae phenotype in multiple fruit fly species has been identified, offering a new method for generating sexing strains.
NATURE COMMUNICATIONS
(2021)
Review
Entomology
Marc J. B. Vreysen, Adly M. M. Abd-Alla, Kostas Bourtzis, Jeremy Bouyer, Carlos Caceres, Chantel de Beer, Danilo Oliveira Carvalho, Hamidou Maiga, Wadaka Mamai, Katerina Nikolouli, Hanano Yamada, Rui Pereira
Summary: The Insect Pest Control Section and its laboratory aim to develop and implement the sterile insect technique (SIT) to reduce the use of insecticides, protect the environment, improve human health, and facilitate international trade. Research conducted between 2010 and 2020 focused on genetic sexing strains, performance assessment, taxonomy, microbiota, genomics, radiation biology, and development of SIT packages. Challenges for the future include making SIT more cost-effective and addressing exotic species introduced by globalization and climate change.
Article
Entomology
Katerina Nikolouli, Fabiana Sassu, Spyridon Ntougias, Christian Stauffer, Carlos Caceres, Kostas Bourtzis
Summary: The study evaluated using Enterobacter sp. AA26 as a protein source in Drosophila suzukii larval diet to replace inactive brewer's yeast, and discussed its effects on the biological quality of the fruit flies.
Article
Biotechnology & Applied Microbiology
Antonios A. Augustinos, Katerina Nikolouli, Lucia Duran de la Fuente, Muhammad Misbah-ul-Haq, Danilo O. Carvalho, Kostas Bourtzis
Summary: This study investigates the effect of genomic background on the efficiency of the sterile insect technique (SIT) using Ae. aegypti genetic sexing strains (GSS). The results show that the genomic background does not negatively affect the inversion result, indicating that the key elements of the GSS can be introgressed into the local genomic background. This finding has implications for improving the application of SIT and addressing regulatory concerns.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)