Article
Agricultural Engineering
Rachapudi Sreeharsha, G. Sai Tejaswini, S. Venkata Mohan
Summary: This study focuses on lipid and volatile fatty acid (VFA) production through dual-stage fermentation of spentwash in a biorefinery framework with Trichosporon cutaneum (Tc) and Yarrowia lipolytica (Yl). The results showed that Tc and Yl accumulated dry biomass of 2.9 and 2.5 g/L respectively in acidhydrolysed spentwash (AHSW) and produced total VFA of 16 and 5.5 g/L respectively. The lipid yields and titres were higher in Tc/AHSW compared to Yl, indicating the effectiveness of Tc in spentwash bioremediation. Furthermore, the analysis of fatty acid composition revealed the presence of higher oleic acid, which is ideal for biodiesel production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Luhuan Sun, Shuai Shao, Jie Bao
Summary: This study found that oleaginous yeast Trichosporon cutaneum exhibited good tolerance to high salinity after long-term adaptive evolution, with better lipid production in seawater compared to freshwater. Additionally, salty water containing phenol was successfully used for lipid fermentation with complete biodegradation.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Biochemistry & Molecular Biology
Juan Liu, Wenting Zhou, Qiaoning He, Man Zhao, Zhiwei Gong
Summary: High concentration of volatile fatty acids (VFAs) can be directly converted into microbial lipid by a robust oleaginous yeast Trichosporon cutaneum, with the blend of VFAs (acetic, propionic, and butyric acids) showing superior performance for lipogenesis. The study demonstrated that VFAs blend can be efficiently utilized by T. cutaneum for lipid production, indicating its potential for high-quality biodiesel production.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Energy & Fuels
Divyata Vilas Rane, Pratik Prashant Pawar, Annamma Anil Odaneth, Arvind Mallinath Lali
Summary: This study used dilute acid pre-treatment and enzymatic hydrolysis to recover 80% of fermentable sugars from corncobs. The enzymatic hydrolysate was then used for microbial oil production, resulting in high lipid production with excellent biofuel properties. The energy content of the microbial oil was found to be significantly higher than that of corncobs, suggesting the potential application of this method in corncob waste management.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Environmental Sciences
Xiaoyu Ma, Min Zhang, Zhen Gao, Ming Gao, Chuanfu Wu, Qunhui Wang
Summary: Utilizing banana straw hydrolysate and pretreated liquor in Rhodosporidium toruloides fermentation can achieve high biomass and lipid production. Additionally, using ethanol stillage as a nitrogen source can promote lipid accumulation, resulting in biodiesel production meeting Chinese and US standards.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Olga Matatkova, Irena Kolouchova, Kristyna Lokocova, Jana Michailidu, Petr Jaros, Marketa Kulisova, Tomas Rezanka, Jan Masak
Summary: The study found that the biological activity of rhamnolipids depends on their properties and the conditions of biofilm formation. Measurement and monitoring of biofilm growth and eradication under static and dynamic conditions are crucial for understanding biofilm behavior.
Article
Engineering, Chemical
Hsiang-Yen Su, Wei-Hai Lin, Ying-Lin Liang, Hsiang-Hui Chou, Si-Wei Wu, Hui-Ling Shi, Jia-Yu Chen, Ke-Ke Cheng
Summary: By genetically engineering the Enterobacter cloacae genome, the CL82 strain was constructed for the co-production of succinic acid and acetoin. CL82 showed high concentrations and productivities of succinic acid and acetoin from glucose, and could also utilize xylose and corncob hydrolysate for co-production. This engineering strategy provides a practical approach for simultaneous production of two commercial products using renewable feedstocks, reducing the cost of microbial fermentation.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Agricultural Engineering
Yasmi Louhasakul, Benjamas Cheirsilp
Summary: This study aimed to convert crude glycerol to lipid and lipase using Yarrowia lipolytica and directly transesterify wet yeast into biodiesel via response surface methodology. The combination of waste and surfactants improved yeast growth, lipid productivity, and lipase activity. The direct transesterification followed by acid-catalyst transesterification offered high FAME yields (>90%), showing the efficiency of the process for industrial biodiesel production from microbial lipids.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jia Zhang, Teng Xu, Xiaohang Wang, Xiaoyan Jing, Jia Zhang, Jiong Hong, Jian Xu, Jichao Wang
Summary: This study describes a process for converting corncob waste into xylitol and identifies the optimal enzyme genes and fermentation conditions for achieving the highest yield and purity of xylitol from corncob hydrolysate.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Chemical
Zakir Hussain, Joji Reddy Lingareddy, Nazleen Sulthana, Shashi Kanth Boddu
Summary: In this study, a functionalized catalyst was designed and synthesized for continuous biodiesel production. The use of a corncob-based solid acid catalyst showed high activity and strong affinity for oleic acid in the esterification reaction.
CHEMICAL ENGINEERING & TECHNOLOGY
(2023)
Article
Agricultural Engineering
Yaning Xu, Xuemei Wang, Zifu Li, Shikun Cheng, Jiacheng Jiang
Summary: Food waste hydrolysate shows great potential as a substrate for microbial oil synthesis, producing biological products with comparable lipid content to synthetic VFAs media. The optimal ratio for microbial oil synthesis is 20:5:5, with an increase in propionic acid proportion key to obtaining odd fatty acids.
BIORESOURCE TECHNOLOGY
(2022)
Article
Energy & Fuels
Stefano Cianchetta, Enrico Ceotto, Stefania Galletti
Summary: This study aimed to evaluate the microbial oil production by three selected strains, Mortierella isabellina, Cunninghamella echinulata, and Thamnidium elegans, using enzymatic hydrolysate from alkali pre-treated giant reed biomass and a semi-synthetic medium. M. isabellina showed the fastest sugar consumption, highest final cell and oil concentrations, as well as the highest cellular oil content, oil yield/g sugar consumed, and oil productivity in the giant reed hydrolysate. Oleic acid was the most abundant fatty acid in the oil, while γ-linolenic acid was highest in C. echinulata and T. elegans.
Article
Energy & Fuels
Marina Grubisic, Maja Galic Perecinec, Ines Peremin, Katarina Mihajlovski, Suncica Beluhan, Bozidar Santek, Mirela Ivancic Santek
Summary: This study focused on the production of lipids from lignocellulosic biomass for biodiesel production, using corn cobs as a carbon source for growth of oleaginous yeast. The results demonstrated that pretreated corn cobs and undetoxified filtrates were suitable carbon sources for the efficient accumulation of lipids in T. oleaginosus.
Article
Biotechnology & Applied Microbiology
Jinbao Yin, Chen Wang, Yilian Li, Bo Lv, Haosheng Lv, Yuyuan Xue, Jing Wu, Jianan Zhang
Summary: In this study, an inhibitor-tolerant strain of A. niger was used for the biological detoxification of corncob hydrolysate. The results showed that A. niger M13 could tolerate high concentrations of acetic acid, furfural, and HMF, and exhibited higher detoxification efficiency in spore form. It could also accumulate citric acid directly from undetoxified hydrolysate.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Hironaga Akita, Mohd Zulkhairi Mohd Yusoff, Shinji Fujimoto
Summary: The study focused on preparing hydrolysate from OPEFB through hydrothermal treatment, with hemicellulose yield decreasing from 153 mg/g-OPEFB to 27.5 mg/g-OPEFB as the temperature increased from 180 to 200 degrees C. The highest glucose yield was obtained from the enzyme-treated solid phase after hydrothermal treatment at 200 degrees C.
FERMENTATION-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Hung-Jen Wang, Pradeep Tyagi, Tsu-Kung Lin, Chao-Cheng Huang, Wei-Chia Lee, Michael B. Chancellor, Yao-Chi Chuang
Summary: This study examined the effects of low energy shock wave (LESW) on bladder and mitochondrial function in a rat model of HCl induced cystitis. The results showed that LESW treatment suppressed bladder overactivity induced by HCl, reduced inflammation and improved mitochondrial function.
BIOMEDICAL JOURNAL
(2022)
Article
Computer Science, Information Systems
Lianghao Xia, Chao Huang, Yong Xu, Huance Xu, Xiang Li, Weiguo Zhang
Summary: As deep learning techniques are applied to real-world recommendation tasks, many deep neural network based Collaborative Filtering (CF) models have been developed. However, most existing models are not well equipped to handle missing data. In this paper, we propose a Collaborative Reflection-Augmented Autoencoder Network (CRANet) that can leverage both observed and unobserved user-item interactions to improve recommendation performance. We also introduce a robust joint training algorithm using regularization-based tied-weights. Experimental results show that debiasing negative signals improves the performance compared to state-of-the-art techniques.
ACM TRANSACTIONS ON INFORMATION SYSTEMS
(2022)
Article
Engineering, Mechanical
Fei Lai, Chaoqun Huang, Xin Ye
Summary: In this paper, a unified dynamics model with 18 degrees of freedom for vehicle chassis is constructed. The influences of factors such as front wheel steering angle, road adhesion coefficient, braking mode of front and rear wheels, and initial speed on vehicle driving stability are analyzed. Furthermore, the influence of uneven road on vehicle driving stability is studied using phase plane and bifurcation analysis methods for the first time.
INTERNATIONAL JOURNAL OF AUTOMOTIVE TECHNOLOGY
(2022)
Article
Clinical Neurology
Fu Li, Xu Lu, Yaoying Ma, Yue Gu, Ting Ye, Chao Huang
Summary: MPL, through inhibiting innate immune stimulation, can prevent stress-induced depression-like behaviors in mice by reducing neuroinflammatory responses.
INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Immunology
Fu Li, Haitao Xiang, Yue Gu, Ting Ye, Xu Lu, Chao Huang
Summary: Pre-stimulation of the innate immune system by MPL can prevent chronic stress-induced anxiety-like behaviors and neuroinflammatory responses in the brain in mice.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Oncology
Chin-Chou Wang, Kuo-Tung Huang, Huang-Chih Chang, Chia-Cheng Tseng, Chien-Hao Lai, Jui Lan, Ting-Ting Liu, Chao-Cheng Huang, Meng-Chih Lin
Summary: The study aimed to assess PD-L1 expression in NSCLC patients with different histological types, EGFR and ALK status, and tumor tissue storage time. There were trends or significant differences in PD-L1 expression among these subgroups, with a higher survival benefit observed in patients without PD-L1 expression in EGFR and ALK mutation patients.
Article
Chemistry, Physical
Rui Jing, Huanzhi Zhang, Chaowei Huang, Fucai Su, Bojing Wu, Zongxu Sun, Fen Xu, Lixian Sun, Yongpeng Xia, Hongliang Peng, Xiangcheng Lin, Bin Li, Yongjin Zou, Hailiang Chu, Pengru Huang, Erhu Yan
Summary: Double cross-linking networks were constructed in PEG-based polymeric SSPCMs by introducing GO and hydroxylated h-BN, which improved the phase-change behavior and thermal conductivity. The SSPCMs exhibited good phase-change properties, excellent thermal reliability, and high thermal conductivity. The study represents a creative strategy for the development of SSPCMs for thermal energy storage and management applications.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Neurosciences
Ruiting Shi, Huijun Liu, Pingping Tan, Zhichao Hu, Yaoying Ma, Minxiu Ye, Yue Gu, Yue Wang, Ting Ye, Yiming Gu, Xu Lu, Chao Huang
Summary: Anxiety is a common psychological disease, and finding methods to prevent anxiety attacks is crucial. This study found that pre-stimulation of the innate immune system can prevent the development of anxiety-like behaviors and reduce neuroinflammatory responses in the brain.
Article
Neurosciences
Wei Tu, Fangfang Fu, Linglong Kong, Bei Jiang, Dana Cobzas, Chao Huang
Summary: Studying functional brain connectivity is crucial for understanding brain function and neuropsychological diseases. This paper proposes a pipeline that estimates robust and stable connectivity matrix, enhancing detectability of group differences. The pipeline is successfully applied to Alzheimer's disease research, showing accurate and stable results compared to traditional methods using original BOLD signals.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Genetics & Heredity
Man-Gang Lee, Yung-Kuo Lee, Shih-Chung Huang, Chen-Lin Chang, Chou-Yuan Ko, Wen-Chin Lee, Tung-Yuan Chen, Shiow-Jyu Tzou, Cheng-Yi Huang, Ming-Hong Tai, Yu-Wei Lin, Mei-Lang Kung, Ming-Chao Tsai, Yung-Lung Chen, Yi-Chen Chang, Zhi-Hong Wen, Chao-Cheng Huang, Tian-Huei Chu
Summary: This study uses bioinformatics analysis to investigate the role of DLK2 in ccRCC. The results suggest that DLK2 may serve as a novel biomarker and therapeutic target for ccRCC.
Article
Biochemical Research Methods
Qin Sun, Xun Bao, Qu Liang, Wei Xu, Qiangling Zhang, Xue Zou, Chaoqun Huang, Chengyin Shen, Yannan Chu
Summary: In this study, a fast gas chromatography proton transfer reaction mass spectrometry (FastGC-PTR-MS) equipment was developed by combining the advantages of rapid detection of proton transfer reaction mass spectrometry (PTR-MS) with the separation and qualitative capabilities of gas chromatography-mass spectrometry (GC-MS). The equipment was used for the rapid qualitative and quantitative detection of butanol isomers in latex paint, showing great potential in indoor environmental safety applications.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Pharmacology & Pharmacy
Hongxiang Hong, Xu Lu, Qun Lu, Chao Huang, Zhiming Cui
Summary: Sinomenine, a natural compound from Sinomenium acutum, has shown beneficial effects in various CNS disorders, including cerebral ischemia, traumatic brain injury, Alzheimer's disease, Parkinson's disease, and epilepsy. Its mechanisms of action involve inhibition of oxidative stress, neuroinflammation, and neuronal apoptosis. Sinomenine also exhibits anti-glioma effects and improves morphine dependence and sleep disturbance.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Clinical Neurology
Chao Huang, Zhangheng Huang, Zichuan Ding, Zongke Zhou
Summary: This study constructed and validated a clinical nomogram to predict cancer-specific survival (CSS) in postoperative patients with primary spinal and pelvic sarcomas. It could assist clinicians in classifying these patients into different mortality risk subgroups and realize sarcoma-specific management.
GLOBAL SPINE JOURNAL
(2022)
Article
Engineering, Civil
Peng Hang, Chao Huang, Zhongxu Hu, Chen Lv
Summary: This paper proposes a game-theoretic decision-making framework to address the coordination issue of connected automated vehicles (CAVs) in urban scenarios. By designing algorithms for driving risk assessment, decision-making cost function, and decision-making constraints, collaborative decisions of vehicles at unsignalized intersections are achieved.
IEEE TRANSACTIONS ON INTELLIGENT TRANSPORTATION SYSTEMS
(2022)
Article
Automation & Control Systems
Tianci Yang, Carlos Murguia, Chen Lv, Dragan Nesic, Chao Huang
Summary: This study addresses the problem of robust state reconstruction for discrete-time nonlinear systems when the actuators and sensors are injected with potentially unbounded attack signals. By exploiting redundancy in sensors and actuators and using a bank of unknown input observers (UIOs), the proposed observer-based estimator is capable of providing asymptotic estimates of the system state and attack signals under the condition that the numbers of sensors and actuators under attack are sufficiently small. Methods for isolating the compromised actuators and sensors are provided using the proposed estimator.
IEEE CONTROL SYSTEMS LETTERS
(2022)
