期刊
BIOTECHNOLOGY LETTERS
卷 34, 期 8, 页码 1475-1482出版社
SPRINGER
DOI: 10.1007/s10529-012-0916-5
关键词
Biofuels; Cellulase; Enzyme recycle; Lignocellulose; Precipitation
资金
- project Demonstrating Industrial scale second generation bioethaol production-Kalundborg Cellulosic Ethanol Plant under the EU FP7 framework program
- project Development of improved second generation (2G) bioethanol technology to prepare for commercialization under the Danish Energy Technology and Demonstration Programme (EUDP)
Comparative studies between commercial Trichoderma reesei cellulase preparations show that, depending on the preparation and loading, total protein precipitation can be as high as 30 % under standard hydrolysis conditions used for lignocellulosic materials. ATR-IR and SDS-PAGE data verify precipitates are protein-based and contain key cell wall hydrolyzing enzymes. Precipitation increased considerably with incubation temperature; roughly 50-150 % increase from 40 to 50 A degrees C and 800 % greater at 60 A degrees C. All of the reported protein losses translated into significant, and often drastic, losses in activity on related 4-nitrophenyl substrates. In addition, supplementation with the non-ionic surfactant PEG 6,000 decreased precipitation up to 80 % in 24 h precipitation levels. Protein precipitation is potentially substantial during enzymatic hydrolysis of lignocelluloses and should be accounted for during lignocellulose conversion process design, particularly when enzyme recycling is considered.
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