期刊
BIOTECHNOLOGY LETTERS
卷 34, 期 2, 页码 261-268出版社
SPRINGER
DOI: 10.1007/s10529-011-0746-x
关键词
Acanthopagrus schlegelii; Polyunsaturated fatty acid elongase; Saccharomyces cerevisiae
资金
- National Academy of Agricultural Science, the Rural Development Administration, Republic of Korea [PJ006745]
To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate gamma-linolenic acid (18:3 n-6) to di-homo-gamma-linolenic acid (20:3 n-6). In addition, the transformed cells converted arachidonic acid (20:4 n-6) and eicosapentaenpic acid (20:5 n-3) to docosatetraenoic acid (22:4 n-6) and docosapentaenoic acid (22:5 n-3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C-18 Delta 6/C-20 Delta 5 but not C-22 PUFA substrates.
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