期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 6, 页码 1177-1184出版社
SPRINGER
DOI: 10.1007/s10529-011-0552-5
关键词
Gene expression; qRT-PCR; Reference genes; Xanthomonas citri subsp citri
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (Fapesp)
- Fundo de Defesa da Citricultura (Fundecitrus)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.
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