期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 4, 页码 829-836出版社
SPRINGER
DOI: 10.1007/s10529-010-0488-1
关键词
Chondrocyte; De-differentiation; Redifferentiation; Spinner-flask culture
资金
- National Research Foundation of Korea, Republic of Korea [2009-0086518, 2009-0092213]
- National Research Foundation of Korea [2009-0092213, 2009-0086518] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据