期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 3, 页码 509-515出版社
SPRINGER
DOI: 10.1007/s10529-010-0465-8
关键词
Bioethanol; Ethanol tolerance; Unsaturated fatty acid
资金
- Ministry of Education, Science and Technology [2009-0081512]
- Ewha Womans University
- National Research Foundation of Korea [2008-2000110] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The effect of change in unsaturated fatty acid composition on ethanol tolerance in Saccharomyces cerevisiae overexpressing ScOLE1 (a dagger 9 fatty acid desaturase gene of S. cerevisiae), CaFAD2 (a dagger 12 fatty acid desaturase gene of Candida albicans), or CaFAD3 (omega 3 fatty acid desaturase gene of C. albicans) was examined. ScOLE1 over-expression increased the total unsaturated fatty acid content and enhanced ethanol tolerance, compared with a control strain. In contrast, overexpression of CaFAD2 and CaFAD3, which led to production of linoleic acid (18:2) and alpha-linolenic acid (18:3), respectively, neither changed total unsaturated fatty acids nor enhanced ethanol tolerance. The total unsaturated fatty acid content rather than the degree of unsaturation is thus an important factor for ethanol tolerance.
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