期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 5, 页码 669-673出版社
SPRINGER
DOI: 10.1007/s10529-009-0196-x
关键词
Antibacterial peptide; Cecropin B; Fusion expression; Green fluorescent protein; Antiserum
Antibacterial peptides have a broad range of antibacterial properties that makes them highly toxic for expression in Escherichia coli. For prepare an antiserum to detect these peptides, we developed a cecropin B mutant with a green fluorescent protein fusion partner resulting in high expression of a 37 kDa fusion peptide in E. coli with a yield of 7.9 mg/l culture medium after purification on Ni-IDA resin. Guinea pigs when immunized with the fusion peptide produced a specific antiserum which titers in excess of 1:25,600.
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