期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 9, 页码 1265-1270出版社
SPRINGER
DOI: 10.1007/s10529-010-0317-6
关键词
Bioreporter; Escherichia coli; Inverted repeat sequence; MopR; mphK promoter
资金
- National Basic Research (973) Program of China [2007CB707805]
- National High-Tech (863) Program of China [2007AA021304]
An Escherichia coli bioreporter harboring the phenol-inducible mphK promoter (P-mphk) from Acinetobacter calcoaceticus PHEA-2 fused to a beta-galactosidase gene (lacZ) and the regulator gene (mopR) of A. calcoaceticus NCIB8250 was constructed to detect phenol. P-mphk containing three inverted repeats (IR1, IR2 and IR3) upstream of mphK was activated by the regulator MopR in the presence of phenol. Deletion analysis of P-mphk revealed that IR2 and IR3 were essential for promoter activity, while IR1 was involved in transcriptional repression. The sensitivity of the bioreporter for the detection of phenol (0.1-5 mu M) was improved by about 100% through deletion of IR1 in P-mphk.
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