期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 8, 页码 1077-1082出版社
SPRINGER
DOI: 10.1007/s10529-010-0259-z
关键词
Aldehyde dehydrogenase II; Ethanol production; Glycerol; Hansenula polymorpha; Pyruvate decarboxylase
资金
- KRIBB Research Initiative
- National Research Council of Science & Technology (NST), Republic of Korea [KGM2420913] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. The ethanol yield was 3.3-fold higher (2.74 g l(-1)) in the engineered yeast compared with the parent strain (0.83 g l(-1)). Further engineering to stimulate glycerol utilization in the recombinant strain via expression of dhaD and dhaKLM genes from Klebsiella pneumoniae encoding glycerol dehydrogenase and dehydroxyacetone kinase, respectively, resulted in a 3.7-fold increase (3.1 g l(-1)) in ethanol yield.
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