期刊
BIOTECHNOLOGY LETTERS
卷 31, 期 12, 页码 1929-1936出版社
SPRINGER
DOI: 10.1007/s10529-009-0097-z
关键词
Fermentation; NAD(+) kinase; NADPH/NADP(+) ratio; Thymidine
Intracellular NADPH/NADP(+) ratio in cells grown on various production media with different carbon and nitrogen sources had a positive correlation with the thymidine production. To improve thymidine production in a previously engineered E. coli strain, NAD(+) kinase was overexpressed in it resulting in the NADPH/NADP(+) ratio shifting from 0.184 to 0.267. The [NADH + NADP(+)]/[NAD(+) + NADPH] ratio was, however, not significantly altered. In jar fermentation, 740 mg thymidine l(-1) was produced in parental strain, while 940 mg l(-1) of thymidine was produced in NAD(+) kinase-expressing strain.
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