期刊
BIOTECHNOLOGY LETTERS
卷 31, 期 10, 页码 1595-1599出版社
SPRINGER
DOI: 10.1007/s10529-009-0057-7
关键词
2-Aminobutyric acid; Asymmetric synthesis; Biphasic reaction; Product inhibition; Transaminase
资金
- Korean Ministry of Education
- Yonsei University and Seoul RBD Program [NT080612, KU080657]
Asymmetric synthesis of an unnatural amino acid was demonstrated by omega-transaminase from Vibrio fluvialis JS17. l-2-Aminobutyric acid was synthesized from 2-oxobutyric acid and benzylamine with an enantiomeric excess higher than 99%. The reaction showed severe product inhibition by benzaldehyde, which was overcome by employing a biphasic reaction system to remove the inhibitory product from the aqueous phase. In a typical biphasic reaction (50 mM 2-oxobutyric acid, 70 mM benzylamine and 2.64 U/ml purified enzyme) using hexane as an extractant, conversion of 2-oxobutyric acid reached 96% in 5 h whereas only 39% conversion was obtained without the product extraction.
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