期刊
BIOTECHNOLOGY LETTERS
卷 31, 期 10, 页码 1633-1637出版社
SPRINGER
DOI: 10.1007/s10529-009-0043-0
关键词
Cell culture; Elicitation; Secondary metabolite; Silymarin
资金
- Ministerio de Ciencia e Innovacion [BFU2008-02876/BFI]
- Junta de Castilla y Leon, Spain [SA009A06]
Production of silymarin and the effect of the elicitor, methyl jasmonate (MeJA), was monitored in cell cultures of Silybum marianum over 4 years. Silymarin concentrations gradually declined after prolonged subculture, making the success of elicitor strategy limited in long-term cultures. The continuous presence of MeJA in cultures for an extended period was necessary for induction of silymarin accumulation. A repeated elicitor strategy was not a good option for improving silymarin productivity in batch cultures. Removal of medium from elicited cultures and addition of fresh medium avoided the toxic effects of elicitor accumulation, allowing the system to respond to a repeated MeJA treatment without loss of productivity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据