期刊
BIOTECHNOLOGY LETTERS
卷 30, 期 10, 页码 1807-1810出版社
SPRINGER
DOI: 10.1007/s10529-008-9762-x
关键词
enantioselective hydrolysis; epoxide hydrolase; glycerol; surfactant
资金
- Ministry of Land, Transportation and Maritime Affairs
- Ministry of Commerce, Industry and Energy, Republic of Korea
The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatalyst. Enantiopure (S)-styrene oxide with 98% ee was obtained with 41% yield (maximum yield = 50%) from 1.8 M racemic styrene oxide at pH 8.0, 4 degrees C in the presence of 40% (v/v) Tween 20 and 5% (v/v) glycerol.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据