期刊
BIOTECHNOLOGY LETTERS
卷 31, 期 3, 页码 443-448出版社
SPRINGER
DOI: 10.1007/s10529-008-9885-0
关键词
beta-Hydroxy amino acid L-threo-2,3-Dihydroxyphenylserine; L-Threonine aldolase; Whole-cell conversion
资金
- New Energy and Industrial Technology Development Organization ( NEDO) in Japan
- Chonbuk National University
l-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing l-threonine aldolase (l-TA) genes cloned from Streptomyces avelmitilis MA-4680. Maximum conversion rates were observed at 2 M glycine, 145 mM 3,4-dihydroxybenzaldehyde, 0.75% Triton-X, 5 g E. coli cells/l, pH 6.5 and 10A degrees C. In the optimized condition, overall productivity was 8 g/l, which represents 40 times the synthesis yield possible with no optimization of conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据