期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 978, 期 -, 页码 131-137出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2014.11.037
关键词
Spermine; Spermidine; Apoptosis; Food; MALDI-TOF MS; NanoUPLC-MS/MS
资金
- Ministry of Science and Technology-Taiwan [MOST 103-2113-M-037-001]
- National Science Council-Taiwan [NSC 102-2113-M-037-010]
- NSYSUKMU Joint Research Project [NSYSUKMU 103-P004]
- Teaching and Learning Excellence Program [U19604]
- Research Center for Environmental Medicine (Kaohsiung Medical University) [KMU-TP103A031]
Spermine and spermidine are multiple-nitrogen compounds found in many foods. Both compounds are essential for cell growth and human health. This study established a simple and fast method of detecting spermine and spermidine in food samples by matrix-assisted laser desorption/ionization combined with time-of-flight mass spectrometry (MALDI-TOF MS). After a simple sample preparation procedure, spermine and spermidine were directly detected by MALDI-TOF MS with no additional purification procedure. The calibration curves for spermine and spermidine ranged from 0.1 to 10 mu g/mL. In intra- and inter-batch assays of three different concentrations of spermine and spermidine, all relative standard deviations and relative errors were below 18.9%. These experimental results confirmed the practicability and effectiveness of the proposed MALDI-TOF MS method for fast determination of spermine and spermidine in food samples. Furthermore, since spermine and spermidine have important roles in apoptosis, up-regulation and down-regulation of spermine and spermidine during apoptosis were analyzed. After treating NRK-52E cells with spermine and spermidine, the cells were lysed, and cell proteins were collected, and digested. Apoptosis-related proteins were then identified by tandem MS. (C) 2014 Elsevier B.V. All rights reserved.
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