Review
Engineering, Environmental
Arkodip Mandal, Kaushik Chatterjee
Summary: 4D bioprinting using water as a stimulus shows great potential in tissue engineering by addressing the challenges of smart hydrogels and cytocompatible stimuli.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Zhiyuan Kong, Xiaohong Wang
Summary: Large diameter artery defects can be substituted by unbiodegradable polymers, while small diameter blood vessels and microvessels pose challenges in constructing complex vascular models. Vascularization strategies can be categorized into self-generated capillaries from implantation, pre-constructed vascular channels, and 3D printed cell-laden hydrogels. This review discusses the latest advances in 3D bioprinting of vascularized tissues/organs, including new printing techniques and bioinks for promoting angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Engineering, Biomedical
Guy Decante, Joao B. Costa, Joana Silva-Correia, Maurice N. Collins, Rui L. Reis, J. Miguel Oliveira
Summary: Three-dimensional (3D) bioprinting technology has gained significant attention in biomedical engineering and clinical applications, with advancements in bioink development allowing for more precise mimicry of native tissue characteristics and support for biofunctionality.
Review
Engineering, Biomedical
X. B. Chen, A. Fazel Anvari-Yazdi, X. Duan, A. Zimmerling, R. Gharraei, N. K. Sharma, S. Sweilem, L. Ning
Summary: Bioinks combine biomaterials and living cells, and are used in extrusion bioprinting to create 3D structures that resemble human tissue or organs. These printed constructs have various applications in tissue engineering and in vitro tissue modeling. The properties of the bioinks, including rheological, mechanical, and biological properties, as well as the printing process, play a crucial role in successful printing and application. This article critically reviews the latest developments in bioinks and biomaterial solutions for extrusion bioprinting, emphasizing bioink synthesis, characterization, and their influence on the printing process.
BIOACTIVE MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Maedeh Rahimnejad, Sepideh Jahangiri, Shahrzad Zirak Hassan Kiadeh, Seyedkamaladdin Rezvaninejad, Zarrin Ahmadi, Sepideh Ahmadi, Moein Safarkhani, Navid Rabiee
Summary: 3D bioprinting is an advanced technology that combines cells and bioactive molecules within a bioscaffold, but it lacks the ability to change or grow in response to a dynamic environment. The emerging field of 4D bioprinting, utilizing smart polymers and hydrogels, adds the dimension of time to 3D bioprinting, allowing for the fabrication of tissue-like structures that can change and reform as tissues are transformed. This has potential applications in biosensing, wound healing, soft robotics, drug delivery, and tissue engineering, but more research is needed to advance this field.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Engineering, Biomedical
Reza Noroozi, Zia Ullah Arif, Hadi Taghvaei, Muhammad Yasir Khalid, Hossein Sahbafar, Amin Hadi, Ali Sadeghianmaryan, Xiongbiao Chen
Summary: Bioprinting is a revolutionary technology in additive manufacturing that prints 3D cell-laden constructs for various clinical applications. It uses biomaterials and cells to regenerate tissues like heart, bone, cornea, cartilage, nerve, and skin, and also contributes to drug delivery, wound healing, bio-actuators, bio-robotics, and bio-sensors. The development of 4D bioprinting and stimuli-responsive materials has further enhanced its potential and led to a billion-dollar market. This study reviews the concepts, applications, and future research topics of 3D and 4D bioprinting, emphasizing the need for collaboration among clinicians, engineers, scientists, and regulatory bodies to translate this technology into biomedical systems.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Manufacturing
Frederik Wulle, Oliver Gorke, Sarah Schmidt, Maximilian Nistler, Gunter E. Tover, Oliver Riedel, Alexanser Verl, Achim Weber, Alexanser Southan
Summary: This paper introduces the potential and advantages of multi-axis 3D printing of hydrogels, and demonstrates through experiments the relative flexibility and water absorbency of 3D printed hydrogels compared to conventional molded hydrogels. The research also highlights related research questions, such as cross-linking chemistry and formulation of hydrogel inks.
ADDITIVE MANUFACTURING
(2022)
Review
Biotechnology & Applied Microbiology
Earnest P. Chen, Zeren Toksoy, Bruce A. Davis, John P. Geibel
Summary: The aim of tissue engineering with 3D bioprinting is to construct fully functional tissue and organ replacements for clinical applications. One of the main challenges is maintaining tissue viability through the inclusion of complex vascular networks. Recent advancements have made great strides in incorporating vascular networks in 3D printed tissue and organs, offering new possibilities for creating biologically functional tissues and organs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Shukufe Amukarimi, Zahra Rezvani, Neda Eghtesadi, Masoud Mozafari
Summary: The development of smart biomaterials has led to enhanced medical facilities in this century. One shining example is the rise of four-dimensional bioprinting technology, which can fabricate stimuli-responsive cell-laden structures that can undergo morphological changes in a pre-planned way.
Review
Biotechnology & Applied Microbiology
Claire Benwood, Josie Chrenek, Rebecca L. Kirsch, Nadia Z. Masri, Hannah Richards, Kyra Teetzen, Stephanie M. Willerth
Summary: Extrusion bioprinting, the most common form of bioprinting, can create personalized tissues for drug testing and cancer research using various materials. This paper reviews the properties and functions of natural bioinks in hard and soft tissue engineering, highlighting the importance of multi-component bioinks and potential future research directions.
BIOENGINEERING-BASEL
(2021)
Article
Polymer Science
Hadis Gharacheh, Murat Guvendiren
Summary: Composite hydrogel bioink, formulated by incorporating human allograft bone particles in a methacrylated alginate formulation, enhances osteogenesis of adult human mesenchymal stem cells (hMSCs) for bone tissue engineering. The bioink is suitable for extrusion-based 3D bioprinting, and in vitro studies show significantly enhanced osteogenic differentiation of hMSCs cultured within the bioprinted composite scaffolds.
Article
Chemistry, Multidisciplinary
Hyeonji Kim, Byeongmin Kang, Xiaolin Cui, Se-Hwan Lee, Kwangseok Lee, Dong-Woo Cho, Woonbong Hwang, Tim B. F. Woodfield, Khoon S. Lim, Jinah Jang
Summary: The new light-activated dECM bioinks with ruthenium/sodium persulfate (dERS) introduced in this study have improved printing performance and mechanical properties, enabling the fabrication of complex constructs with increased shape fidelity, expanding the biofabrication possibilities.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Jianhua Zhang, Esther Wehrle, Marina Rubert, Ralph Mueller
Summary: Three-dimensional (3D) bioprinting is a promising approach for tissue engineering, allowing precise placement of cells and biomaterials to mimic complex microstructures of biological tissues. Bioprinted tissues can benefit from exposure to fluid mechanical cues provided by bioreactor systems, aiding in tissue development and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Sara Simorgh, Neda Alasvand, Mahboobe Khodadadi, Faezeh Ghobadi, Maziar Malekzadeh Kebria, Peiman Brouki Milan, Saeid Kargozar, Francesco Baino, Ali Mobasheri, Masoud Mozafari
Summary: Tissue engineering and regenerative medicine use additive manufacturing technology for 3D printing, with bioactive glasses being one of the most promising raw materials that can improve the bioactivity and antibacterial activity of constructs. This review provides an up-to-date report on the development of 3D printed scaffolds using BG-containing raw biomaterials for tissue regeneration applications, focusing on their advantages and challenges.
Review
Chemistry, Multidisciplinary
Mohamadmahdi Samandari, Jacob Quint, Alejandra Rodriguez-delaRosa, Indranil Sinha, Olivier Pourquie, Ali Tamayol
Summary: Skeletal muscle injuries can lead to mobility limitations and loss of independence, with current treatments showing variable effectiveness. Skeletal muscle tissue engineering and bioprinting are considered promising solutions for reconstructing complex muscle structures and improving functional recovery.
ADVANCED MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Hossein Montazerian, Elham Davoodi, Avijit Baidya, Sevana Baghdasarian, Einollah Sarikhani, Claire Elsa Meyer, Reihaneh Haghniaz, Maryam Badv, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Hemostatic biomaterials have great potential in wound control, particularly for uncontrolled bleeding associated with damaged tissues, traumatic wounds, and surgical incisions. Current research focuses on enhancing the hemostatic properties of bioactive materials through triggering the coagulation cascade, using biocompatible and biodegradable materials for rapid blood coagulation, and developing tough bioadhesives for efficient incision sealing.
Article
Engineering, Biomedical
Edna Johana Bolivar-Monsalve, Carlos Fernando Ceballos-Gonzalez, Carolina Chavez-Madero, Brenda Guadalupe de la Cruz-Rivas, Silvana Velasquez Marin, Shirley Mora-Godinez, Luisa Maria Reyes-Cortes, Ali Khademhosseini, Paul S. Weiss, Mohamadmahdi Samandari, Ali Tamayol, Mario Moises Alvarez, Grissel Trujillo-de Santiago
Summary: This study presents an effective and practical method for the fabrication of pre-vascularized engineered tissues. By co-extruding cell-laden hydrogels and sacrificial materials through printheads containing Kenics static mixing elements, thin hydrogel filaments containing dozens of hollow microchannels as small as a single cell are continuously and one-step fabricated. The hollow channels in the filaments promote cell viability, metabolic activity, proliferation, and cell alignment.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Materials Science, Biomaterials
Marvin Mecwan, Reihaneh Haghniaz, Alireza Hassani Najafabadi, Kalpana Mandal, Vadim Jucaud, Johnson V. John, Ali Khademhosseini
Summary: Researchers have successfully engineered a thermoresponsive shear-thinning hydrogel, composed of a thermoresponsive polymer and hemostatic silicate nanodisks, as an injectable hemostat. This hydrogel demonstrates rapid mechanical recovery and temperature-dependent blood coagulation, effectively preventing blood loss. It shows comparable efficacy to a commercially available hemostat and can be easily removed using a cold saline wash.
BIOMATERIALS SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Kasinan Suthiwanich, Masaya Hagiwara
Summary: Localization of different hydrogel conditions in a unit-based scaffold enables spatial control of 3D tissue morphogenesis. This technique allows for the trapping of hydrogel solutions without the need for chemical additives, facilitating the spatial organization of biomolecular compositions and physical conditions within the scaffold. The localization of growth factors or matrix proteins in hydrogels demonstrates the direct dependence of tissue development on local hydrogel conditions. The scaffold-based localization technique is simple to use and has promising applications in biomedical research.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Gang Ge, Kalpana Mandal, Reihaneh Haghniaz, Mengchen Li, Xiao Xiao, Larry Carlson, Vadim Jucaud, Mehmet Remzi Dokmeci, Ghim Wei Ho, Ali Khademhosseini
Summary: Adhesive materials have been receiving increasing attention for their excellent sealing ability. However, existing adhesives typically have weak adhesion strength and deteriorate in harsh environments. In this study, a novel ionogel with tunable mechanical properties is developed using a deep eutectic solvent as the medium for photopolymerization. The ionogel demonstrates fast gelation and bonding without external pressure, high adhesion in various environments, and wide applications in packaging, marine engineering, medical adhesives, and electronic assembly.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Editorial Material
Engineering, Environmental
Unax Lertxundi, Saioa Domingo-Echaburu, Susana Barros, Miguel Machado Santos, Teresa Neuparth, Jose Benito Quintana, Rosario Rodil, Rosa Montes, Gorka Orive
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Pauline Cheng, Ahmad Rashad, Ankit Gangrade, Natan Roberto de Barros, Ali Khademhosseini, Jonathan Tam, Padmini Varadarajan, Devendra K. Agrawal, Finosh G. Thankam
Summary: Myocardial infarction leads to the loss of cardiomyocytes, but stem cell therapy shows promise in restoring cardiac function. This article discusses the application of different stem cell phenotypes and strategies to promote their differentiation into cardiomyocytes.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Engineering, Biomedical
Hossein Montazerian, Alireza Hassani Najafabadi, Elham Davoodi, Rasoul Seyedmahmoud, Reihaneh Haghniaz, Avijit Baidya, Wei Gao, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Mussel-inspired catechol-functionalization of degradable natural biomaterials is proposed as a potential approach to achieve bioadhesion for sutureless wound closure. This approach involves a simple oxidative polymerization step before conjugation of catechol-carrying molecules to amplify catechol function in bioadhesion. The modified gelatin with poly(l-DOPA) moieties shows improved wound control and enhanced cohesion, surpassing commercial sealants, and also possesses photothermal responsiveness and antibacterial activity, making it an effective biomaterial design strategy for wound closure applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Fahimeh Roshanfar, Saeed Hesaraki, Alireza Dolatshahi-Pirouz, Mohsen Saeidi, Sara Leal-Marin, Birgit Glasmacher, Gorka Orive, Sajjad Khan Einipour
Summary: Calcium phosphate cements (CPCs) are promising for bone defect treatment due to their properties, however their brittleness limits their application. This study investigated the use of hybrid silk fibroin (SF) and kappa-carrageenan (k-CG) nanofibers as reinforcements in CPC. The addition of nanofibers improved the mechanical properties and cell mineralization of CPC composites, indicating their potential for bone cell regeneration.
Article
Materials Science, Multidisciplinary
Hossein Montazerian, Shameek Mitra, Alireza Hassani Najafabadi, Rasoul Seyedmahmoud, Yuting Zheng, Mehmet Remzi Dokmeci, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Inspired by mussel adhesion, catechol functionalization has been widely used to impart adhesion to biomaterials. However, the bioadhesion capacity of catechol motifs in hydrogels that crosslink through free-radical-based systems is debated. This study reevaluates the bioadhesion efficacy of catechol functionalized gelatin biomolecules in methacryloyl-modified photo-cross-linkable biomaterials, and investigates the effects of catechol content on adhesion strength and hydrogel integrity. The cytotoxic and immunostimulatory effects of catechol groups are also evaluated for clinical applications.
ACS MATERIALS LETTERS
(2023)
Editorial Material
Environmental Sciences
Iker Egana, Saioa Domingo-Echaburu, Gorka Orive, Unax Lertxundi
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Oncology
Ali Zarrabi, David Perrin, Mahboubeh Kavoosi, Micah Sommer, Serap Sezen, Parvaneh Mehrbod, Bhavya Bhushan, Filip Machaj, Jakub Rosik, Philip Kawalec, Saba Afifi, Seyed Mohammadreza Bolandi, Peiman Koleini, Mohsen Taheri, Tayyebeh Madrakian, Marek J. Los, Benjamin Lindsey, Nilufer Cakir, Atefeh Zarepour, Kiavash Hushmandi, Ali Fallah, Bahattin Koc, Arezoo Khosravi, Mazaher Ahmadi, Susan Logue, Gorka Orive, Stevan Pecic, Joseph W. Gordon, Saeid Ghavami
Summary: Rhabdomyosarcoma is a rare cancer that affects skeletal muscle, primarily in children and young adults. The treatment strategies for this disease include multi-agent chemotherapies following surgical resection and radiotherapy. This review provides a detailed clinical understanding of rhabdomyosarcoma, including its classification, diagnosis, and treatment strategies. The impact of apoptosis, macro-autophagy, and the unfolded protein response on chemotherapy response is discussed. Additionally, the use of in vivo mouse and zebrafish models and in vitro three-dimensional bioengineering models for screening future therapeutic approaches and promoting muscle regeneration is explored.
Article
Chemistry, Analytical
Safoora Khosravi, Saeid Soltanian, Amir Servati, Ali Khademhosseini, Yangzhi Zhu, Peyman Servati
Summary: Wearable sweat biosensors embedded in textile substrates have gained attention for noninvasive monitoring of health parameters. A flexible electrochemical glucose sensor that can be screen-printed onto a textile substrate is demonstrated, achieving a linear response in the range of 20-1000μM of glucose concentration with high sensitivity and stability. These textile-based biosensors show high selectivity towards glucose and have the potential to impact the next generation of wearable devices.
Review
Automation & Control Systems
Baishali Kanjilal, Yangzhi Zhu, Vaishali Krishnadoss, Janitha M. M. Unagolla, Parnian Saemian, Alessia Caci, Danial Cheraghali, Iman Dehzangi, Ali Khademhosseini, Iman Noshadi
Summary: Ionic liquids (ILs) have unique properties that make them promising candidates for biomedical applications, but their cytocompatibility limitations are enhanced by using bioionic liquids (BILs) derived from biological molecules. BILs can be synthesized and immobilized onto biopolymers, and their functionalization enables the design of responsive actuators and sensors. The cytocompatibility of BIL-functionalized polymers also makes them suitable for power storage and implantable devices. This review focuses on the recent advances of BILs in biomedical applications, specifically their use as functionalization agents for biopolymers and solvents for supermolecular ionic networks.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Chemistry, Multidisciplinary
Bruna Gregatti Carvalho, Aya Nakayama, Hiromi Miwa, Sang Won Han, Lucimara Gaziola de la Torre, Dino Di Carlo, Junmin Lee, Han-Jun Kim, Ali Khademhosseini, Natan Roberto de Barros
Summary: A novel mRNA-releasing matrix based on GelMA microporous annealed particle scaffolds is reported. The sustained release of mRNA complexes achieves indirect intracellular delivery, while direct intracellular delivery is achieved by cell adhesion on the mRNA-releasing scaffolds. This hybrid system demonstrates efficient protein expression, offering potential for mRNA-releasing biomaterials in tissue engineering.
