期刊
出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.11.021
关键词
LC-MS/MS; Flavonoids; Paulownia tomentosa; Pharmacokinetic study
资金
- National Nature Science Foundation of China [81303156]
- Natural Science Foundation of Guangdong Province of China [2013040016491]
- Program for Outstanding Young Teachers in Higher Education Commission of Guangdong Province of China [Yq2013085]
A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of six components including apigenin, quercetin, apigenin-7-O-beta-D-glucoside, quercetin-3-O-beta-D-glucoside, 3'-methoxyluteolin-7-O-beta-D-glucoside, and tricin-7-O-beta-D-glucopyranoside in rat plasma using formononetin as the internal standard (IS). The plasma samples were pretreated by a one-step liquid-liquid extraction with dichloromethane. The chromatographic separation was carried out on a ZORBAX SB-Aq column with a gradient mobile phase consisting of acetonitrile and 2 mM aqueous ammonium acetate. All analytes and IS were quantitated through electrospray ionization in negative ion multiple reaction monitoring mode. The mass transitions were as follows: m/z 269.1 -> 117.2 for apigenin, m/z 301.2 -> 151.2 for quercetin, m/z 431.3 -> 311.2 for apigenin-7-O-beta-D-glucoside, m/z 463.2 -> 300.2 for quercetin-3-O-beta-D-glucoside, m/z 461.3 -> 283.1 for 3'-methoxyluteolin-7-O-beta-D-glucoside, m/z 491.3 -> 313.1 for tricin-7-O-beta-D-glucopyranoside, and m/z 267.2 -> 252.2 for IS, respectively. All calibration curves exhibited good linearity with correlation coefficient (r)> 0.995. The intra-day and inter-day precisions (RSD) at three QC levels were both less than 14.0% and the accuracies ranged from 89.8% to 113.8%. The extraction recoveries of six compounds ranged from 82.3% to 92.5%. The validated method was successfully applied to pharmacokinetic study of the six components in male rat plasma after oral administration of Paulownia tomentosa flower extract. (C) 2014 Elsevier B.V. All rights reserved.
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