Article
Chemistry, Analytical
Petr Kozlik, Jana Vaclova, Kveta Kalikova
Summary: In this study, three recently developed HILIC columns were characterized in detail to compare their peptide retention mechanisms, revealing a multimodal retention mechanism in systems with these stationary phases. By balancing the properties of analytes and stationary phases, satisfactory retention and peak shape of peptides can be achieved. Buffer concentration and pH optimization was confirmed to be an effective tool for manipulating retention and peak symmetry, enhancing the potential applications of these columns in the analysis of diverse compounds.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Michal Maly, Zuzana Kadlecova, Pavel Dubsky, Kveta Kalikova
Summary: Mixed-mode chromatography is a widely used analytical method that combines reversed-phase and ion-exchange interactions in one column. Understanding how these interactions affect analyte retention is important for designing effective mixed-mode-based methods. Previous models were limited, but this paper improves upon them by considering weak acids and bases, as well as the effect of organic modifiers.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Qi Wu, Xiudan Hou, Haitao Lv, Hui Li, Liang Zhao, Hongdeng Qiu
Summary: The novel octadecylamine-derived CDs, C18-CDs, were successfully designed, synthesized, and applied in RPLC/HILIC mixed-mode chromatography, showing good separation performance for both hydrophobic and hydrophilic compounds. The C18-CDs/SiO2 column demonstrated efficient separation for various compounds under different modes, indicating its potential for further research in mixed-mode chromatography.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Fei Wang, Fan Yang, Jiawei Liu, Quan Bai
Summary: This study investigates the retention behavior and mechanism of solutes in hydrophilic interaction chromatography (HILIC) using stoichiometric displacement theory (SDT). A HILIC/reversed-phase liquid chromatography (RPLC) dual-retention mechanism is studied in detail using a β-CD HILIC column. The study finds that a four-parameter equation derived from SDT-R accurately describes the U-shaped curves of solutes with RPLC/HILIC dual-retention mechanisms on the β-CD column, providing a theoretical guide for the development of HILIC and the enhancement of separation efficiency.
Article
Materials Science, Multidisciplinary
Nannan Wu, Yalei Wang, Yongle Tong, Yulin Tan, Huaifei Liu, Xiang Xiong, Rutie Liu
Summary: The corrosion behaviors and mechanism of CMAS on Yb2SiO5 (YbMS) ceramics were studied. The results showed that CMAS corroded YbMS and generated various stable apatite and garnet phases, depending on the doping cations. The diffusion of Ca2+ along the grain boundaries and volume of YbMS was observed as the reaction front, resulting in discontinuous reaction layers. The dissolved YbO1.5 diffused along the CMAS glass grid and formed a cyclic apatite phase when the solubility was saturated, above 1300 degrees C.
Article
Chemistry, Analytical
Jin-Cheng Yang, Shuo Gao, Jing-Han Zhang, Hai-Tao Lv, Qi Wu
Summary: In this study, a novel type of carbon dots (CDs) was synthesized using citric acid, 1-aminoethyl-3-methylimidazolium bromide ionic liquid, and octadecylamine. The synthesized CDs possessed multiple functional groups, including carboxyl, amino, hydroxyl, and imidazole groups. A silica gel chromatographic column functionalized with CDs (Si-CDs) was developed and shown to be suitable for separating compounds with different properties. The retention mechanism on the Si-CDs column involved hydrophobic, pi-pi stacking, and electrostatic interactions. Real sample analysis of residual thiamphenicol and chloramphenicol in milk demonstrated the rapidity and reliability of the developed detection method based on this Si-CDs column.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Bohumil Dolensky, Ameneh Tatar, Martin Havlik, Tereza Navratilova, Jan Hajduch, Pavel Matejka
Summary: This study presents a methodology for deconvolution of total solute retention into structure-specific contributions, using different chemically modified silica-based stationary phases to investigate the impact of various structures on solute retention. The results provide insight into the retention mechanism and emphasize the importance of considering structure-specific factors in chromatographic evaluations.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Maria Carmen Prieto-Blanco, Angela Planas-Franco, Soledad Muniategui-Lorenzo, Maria Jose Gonzalez-Castro
Summary: A retention behavior based on mixed-mode reversed-phase (RP)/hydrophilic interaction liquid chromatography (HILIC) was observed for benzalkonium chloride (BAK) using a core-shell column functionalized with biphenyl groups. The behavior was dependent upon the chemical structure of the analyte with mixed functionality and the selectivity of the chromatographic column. Bimodal retention was observed for the four BAK homologues, and the data were modeled using polynomial equations to predict the U-shaped retention.
Article
Biochemical Research Methods
Katarina Molnarova, Ales Duris, Tomas Jecmen, Petr Kozlik
Summary: Glycoproteomics, a challenging field due to glycosylation heterogeneity, benefits from hydrophilic interaction liquid chromatography for glycopeptide separation. Comparing HILIC columns in human immunoglobulin G glycopeptide separation, it was found that different columns exhibit higher separation power for different glycoforms and can effectively elucidate the structure of isomeric glycoforms. The retention mechanism for HILIC columns is discussed based on determined chromatographic parameters.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Ryan Kilgore, Arianna Minzoni, Shriarjun Shastry, Will Smith, Eduardo Barbieri, Yuxuan Wu, Jacob P. Lebarre, Wenning Chu, Juliana O'Brien, Stefano Menegatti
Summary: Viral vectors play a significant role in modern medicine and biotechnology as they are used for gene therapies, oncolytic agents, vaccine platforms, and cell therapies. The success of viral vectors relies on flexible and affordable biomanufacturing strategies to meet the increasing demand worldwide. Downstream process technology, including the discovery of affinity ligands and development of innovative adsorbents, is crucial in ensuring high yield and purity of viral vectors. Novel process layouts and analytical methods are also important in monitoring the quality attributes of viral vectors. This study provides a comprehensive overview of the bioprocess toolbox for viral vectors and discusses future directions for their success.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Fangbin Fan, Xiaofeng Lu, Xiaojing Liang, Licheng Wang, Yong Guo
Summary: The study introduces a new design strategy for dual-network hydrogel, which is successfully applied in liquid chromatography for extended applications. This new stationary phase exhibits high selectivity and chromatographic separation performance, effectively separating various compounds.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Kaixing Luo, Yu Luo, Yanjuan Liu, Yuefei Zhang, Wei Chen, Zhengwu Bai, Sheng Tang
Summary: In this work, a new strategy was adopted to simultaneously graft two different functional ligands onto the silica surface, resulting in a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) using recycled commercial C18 columns as raw materials. The obtained Sil-CDI-DD exhibited enhanced separation selectivity and efficiency, providing multiple interaction sites for diverse target analytes and enabling the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Applied
Solmaz Hajizadeh
Summary: The demand for efficient separation and purification methods has led to the development of new techniques and materials, such as composite cryogels. These composite cryogels can directly purify targets from particulate-containing fluids and offer higher binding capacities, improved selectivity, and enhanced overall performance compared to conventional chromatographic columns.
REACTIVE & FUNCTIONAL POLYMERS
(2023)
Article
Biochemical Research Methods
Jinxin Chi, Dandan Zhu, Yiqiong Chen, Guihua Huang, Xucong Lin
Summary: In this study, a facile and practical aptamer-grafted ionic affinity monolith with mixed-mode mechanism was explored as a versatile platform for online specific recognition of polar and non-polar mycotoxins. The mixed-mode mechanism provided better flexibility in adjusting separation selectivity and reducing nonspecific adsorption compared to single mode. The affinity monolith showed good recoveries of OTA, AFB1, and ZEN in cereals, demonstrating the efficiency and competency of this method for online mycotoxin recognition.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Elham Sadat Hosseini, Kourosh Tabar Heydar
Summary: The research synthesized two acridine derivatives and developed two mixed-mode stationary phases by functionalizing silica. These stationary phases were designed with multiple interactions, including pi-pi stacking interaction, reverse phase, hydrophilic interaction, and anion exchange, based on the structure of the acridine groups on the silica gel. The performance of the columns was investigated by chromatographic parameters in terms of column efficiency, asymmetry factor, retention factor, and resolution.
Article
Engineering, Biomedical
Sabine Rosenfeldt, Frank Mickoleit, Cornelia Joerke, Joachim H. Clement, Simon Markert, Valerie Jerome, Stephan Schwarzinger, Ruth Freitag, Dirk Schueler, Rene Uebe, Anna S. Schenk
Summary: This study characterizes a multi-step purification protocol for bacterial magnetosomes (MS) in terms of purity, iron content, size, and polydispersity. The potential cytotoxic effects of isolated MS when incubated with mammalian cells are also addressed. The study helps establish prerequisites for real-world applications of magnetosomes in biotechnology and biomedicine by providing a detailed overview of the process-structure relationship during the isolation of MS.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Michael Mader, Moritz Helm, Mingxia Lu, Martina H. Stenzel, Valerie Jerome, Ruth Freitag, Seema Agarwal, Andreas Greiner
Article
Chemistry, Multidisciplinary
Valerie Jerome, Christopher V. Synatschke, Ruth Freitag
Article
Microbiology
Nicolas Weithmann, Stanislava Mlinar, Eva Sonnleitner, Alfons Rupert Weig, Ruth Freitag
Summary: Flexible feeding of biogas production can save substrates and storage capacity while maintaining control over electricity production. Adjustment of feeding amounts enables rapid recovery of gas production levels, with a noticeable impact on microbial community dynamics.
Article
Biochemistry & Molecular Biology
Daniel Keim, Katrin Gollner, Ulrich Gollner, Valerie Jerome, Ruth Freitag
Summary: The study demonstrated that a 24-armed PDMAEMA nano-star can achieve high levels of transgene expression (40%) in human B cells while maintaining sufficient cell viability. Optimal transfection results were obtained with four days of stimulation with rhCD40L post-thawing. Furthermore, B cell subsets were found to impact transfection outcomes, highlighting the importance of considering the complexity and heterogeneity of B cell populations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
M. Helm, S. A. B. Riedl, K. Gollner, U. Gollner, V Jerome, R. Freitag
Summary: The study presents a method for isolating primary B cells from both juvenile and adult tonsillar material, achieving purities >= 80% through nylon wool column separation. This method preserves all B cell subsets and provides high number of cells from tonsils, enhancing the comparability of results in subsequent experiments. The isolated B cells demonstrate proliferative capability and show a decline in proliferative response with donor age.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Julia Rudolph, Matthias Voelkl, Valerie Jerome, Thomas Scheibel, Ruth Freitag
Summary: The study found that macrophages can effectively ingest microplastic particles, while epithelial cells did not show significant ingestion of microplastics. Macrophages from two cell lines exhibited high ingestion of microplastics of all sizes, but cytotoxic effects were only observed in one cell line. Cell populations with high particle interactions showed signs of cellular stress and effects on cell proliferation.
SCIENTIFIC REPORTS
(2021)
Article
Ecology
Simon A. B. Riedl, Matthias Voelkl, Anja Holzinger, Julia Jasinski, Valerie Jerome, Thomas Scheibel, Heike Feldhaar, Ruth Freitag
Summary: The intestinal cells of the earthworm Eisenia fetida are suitable for studying the toxic effects of environmental pollutants on a cellular level. Exposure to pollutants and soil elutriates led to a decrease in metabolic activity of the cells. Additionally, microplastic particles did not show active uptake, but had dose and size dependent effects on metabolism that may correlate with organismic effects.
Article
Environmental Sciences
Thomas Steiner, Julia N. Moeller, Martin G. J. Loeder, Frank Hilbrig, Christian Laforsch, Ruth Freitag
Summary: High-caloric-value household biowaste is a desirable substrate for biogas and fertilizer production, but it is often contaminated by plastic packaging. This study found that fertilizer from biowaste treatment plants had significant plastic contamination, while fertilizer from agricultural biogas plants and greenery composts had minimal contamination. The level of contamination appeared to be influenced by operating conditions, with shredding of incoming material increasing the degree of contamination.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Nanoscience & Nanotechnology
Julia Jasinski, Magdalena Wilde, Matthias Voelkl, Valerie Jerome, Thomas Frohlich, Ruth Freitag, Thomas Scheibel
Summary: This study investigates the formation of protein corona on microplastic particles and its influence on particle-cell interactions. The research demonstrates that particle history plays a significant role in protein corona formation and subsequent interactions with murine intestinal epithelial-like cells. Lysozyme preincubation shows the highest rate of particle-cell interactions among the tested proteins.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Agricultural Engineering
Stanislava Mlinar, Alfons R. Weig, Ruth Freitag
Summary: Despite extensive research, the effects of NH3 and NH4+ on anaerobic digestion and associated microorganisms are still not fully understood. This study investigated the effects of different NH3/NH4+ ratios on microorganisms involved in anaerobic digestion of two substrates. It was found that bacteria, particularly gram-positive bacteria, were affected by total ammonia nitrogen (TAN), while archaea were largely influenced by NH3 concentration. Adjusting the NH3/NH4+ ratio can avoid inhibition and stabilize biogas production in nitrogen-rich substrates.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Moritz Helm, Songyan B. Huang, Katrin Gollner, Ulrich Gollner, Valerie Jerome, Ruth Freitag
Summary: Polyelectrolyte microcapsules based on SCS and PDADMAC are investigated for cultivating human primary B cells, showing that the microenvironment provided by the microcapsules accelerates the induction of B cell differentiation, particularly the formation of plasma cells, and supports their long-term survival.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Engineering, Environmental
Yuanhu Zhang, Tasmai Paul, Julian Brehm, Matthias Voelkl, Valeïrie Jerome, Ruth Freitag, Christian Laforsch, Andreas Greiner
Summary: The toxic effects of polystyrene particles are due to residual styrene monomers, which may lead to ambiguous interpretations of their impact on cells, tissues, and organisms. Therefore, it is important to analyze the residual styrene content. A simple and sensitive UV-vis method was developed to analyze residual styrene and polystyrene concentration. The study found that standard polystyrene particles containing residual monomers exhibited low but significant cytotoxicity, while in-house synthesized polystyrene particles with reduced styrene content did not.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Julia Jasinski, Matthias Voelkl, Jonas Hahn, Valerie Jerome, Ruth Freitag, Thomas Scheibel
Summary: The impact of microplastic particles on organisms is currently under intense research. This study investigated the fate of polystyrene particles ingested by murine macrophages, including their distribution during cell division and possible mechanisms of excretion. The results showed that particle distribution during cell division was cell-specific, and no active excretion of microplastic particles was observed. Additionally, polarized macrophages exhibited higher phagocytic activity and particle uptake compared to non-polarized cells. Furthermore, it was found that submicron particles were co-localized with the endoplasmic reticulum, while 0.5μm particles were occasionally found in endosomes.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Ivonne L. Diaz Ariza, Valerie Jerome, Leon D. Perez Perez, Ruth Freitag
Summary: Nonviral gene delivery vectors are less toxic and immunogenic compared to viral ones, but improvements in efficacy are still needed, with challenges in effective complexation, protection, and intracellular dissociation. Research on copolymers with multiple interactive functions has shown that optimizing transfection benefits from treatment as a multiparameter problem.
ACS APPLIED BIO MATERIALS
(2021)