Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing

Plasmid DNA fermentation strain and process-specific effects on vector yield, quality, and transgene expression

(2010) Aaron E. Carnes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Separation of supercoiled from open circular forms of plasmid DNA, and biological activity detection

(2010) Huangjin Li et al.   CYTOTECHNOLOGY

DNA vaccines: an historical perspective and view to the future

(2010) Margaret A. Liu   IMMUNOLOGICAL REVIEWS

Marker-free plasmids for gene therapeutic applications—Lack of antibiotic resistance gene substantially improves the manufacturing process

(2010) Jürgen Mairhofer et al.   JOURNAL OF BIOTECHNOLOGY

pFARs, Plasmids free of antibiotic resistance markers, display high-level transgene expression in muscle, skin and tumour cells

(2010) Corinne Marie et al.   JOURNAL OF GENE MEDICINE

Critical design criteria for minimal antibiotic-free plasmid vectors necessary to combine robust RNA Pol II and Pol III-mediated eukaryotic expression with high bacterial production yields

(2010) Aaron E. Carnes et al.   JOURNAL OF GENE MEDICINE

A robust system for production of minicircle DNA vectors

(2010) Mark A Kay et al.   NATURE BIOTECHNOLOGY

EcoCyc: a comprehensive database of Escherichia coli biology

(2010) I. M. Keseler et al.   NUCLEIC ACIDS RESEARCH

Biosafety of DNA vaccines: New generation of DNA vectors and current knowledge on the fate of plasmids after injection

(2010) Florence Faurez et al.   VACCINE

Engineering of bacterial strains and vectors for the production of plasmid DNA

(2009) Diana M. Bower et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Plasmid DNA vaccine vector design: Impact on efficacy, safety and upstream production

(2009) James A. Williams et al.   BIOTECHNOLOGY ADVANCES

Generic plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes

(2009) James A. Williams et al.   BIOTECHNOLOGY AND BIOENGINEERING

Epigenetic regulation of the bacterial cell cycle

(2009) Justine Collier   CURRENT OPINION IN MICROBIOLOGY

DNA vaccine manufacture: scale and quality

(2009) Ying Cai et al.   Expert Review of Vaccines

Progress and Prospects: The design and production of plasmid vectors

(2009) D R Gill et al.   GENE THERAPY

Pyruvate Kinase-Deficient Escherichia coli Exhibits Increased Plasmid Copy Number and Cyclic AMP Levels

(2009) D. S. Cunningham et al.   JOURNAL OF BACTERIOLOGY

Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress

(2009) Pedro H. Oliveira et al.   JOURNAL OF BIOTECHNOLOGY

DNA plasmid production in different host strains of Escherichia coli

(2009) Adam Singer et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint

(2009) Drew S Cunningham et al.   Microbial Cell Factories

Proteome analysis of the Escherichia coli heat shock response under steady-state conditions

(2009) Svenja Lüders et al.   Proteome Science

Structural instability of plasmid biopharmaceuticals: challenges and implications

(2009) Pedro H. Oliveira et al.   TRENDS IN BIOTECHNOLOGY

Improved antibiotic-free DNA vaccine vectors utilizing a novel RNA based plasmid selection system

(2009) Jeremy Luke et al.   VACCINE

Enhancement of plasmid DNA yields during fed-batch culture of a fruR-knockout Escherichia coli strain

(2008) Dave Siak-Wei Ow et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Modified Escherichia coli B (BL21), a superior producer of plasmid DNA compared with Escherichia coli K (DH5α)

(2008) Je-Nie Phue et al.   BIOTECHNOLOGY AND BIOENGINEERING

Host strain influences on supercoiled plasmid DNA production inEscherichia coli: Implications for efficient design of large-scale processes

(2008) Sin Yee Yau et al.   BIOTECHNOLOGY AND BIOENGINEERING

The effect of heating rate onEscherichia colimetabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: A scale-down study

(2008) Luis Caspeta et al.   BIOTECHNOLOGY AND BIOENGINEERING

The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse

(2008) T. Durfee et al.   JOURNAL OF BACTERIOLOGY

Effect of temperature up-shift on fermentation and metabolic characteristics in view of gene expressions in Escherichia coli

(2008) Chowdhurry Hasan et al.   Microbial Cell Factories

DNA vaccines: ready for prime time?

(2008) Michele A. Kutzler et al.   NATURE REVIEWS GENETICS

Comparative studies on in vitro expression and in vivo immunogenicity of supercoiled and open circular forms of plasmid DNA vaccines

(2007) Vinod Bhaskara Pillai et al.   VACCINE