期刊
BIOTECHNOLOGY JOURNAL
卷 5, 期 11, 页码 1192-1197出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201000202
关键词
Biocatalysis; Cross-linked enzyme aggregate; Enzyme immobilization; Sucrose phosphorylase; Thermostability
资金
- Flanders' Agency for Innovation by Science and Technology (IWT-Vlaanderen) [SBO-50191]
Sucrose phosphorylase is an interesting biocatalyst that can glycosylate a variety of small molecules using sucrose as a cheap but efficient donor substrate. The low thermostability of the enzyme, however, limits its industrial applications, as these are preferably performed at 60 degrees C to avoid microbial contamination. Cross-linked enzyme aggregates (CLEAs) of the sucrose phosphorylase from Bifidobacterium adolescentis were found to have a temperature optimum that is 17 degrees C higher than that of the soluble enzyme. Furthermore, the immobilized enzyme displays an exceptional thermostability, retaining all of its activity after 1 week incubation at 60 degrees C. Recycling of the biocatalyst allows its use in at least ten consecutive reactions, which should dramatically increase the commercial potential of its glycosylating activity.
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