Article
Microbiology
Yidong Liu, Wenxuan Sun, Liting Ma, Rong Xu, Chunyu Yang, Ping Xu, Cuiqing Ma, Chao Gao
Summary: Given the intrinsic and potential resistance of Pseudomonas aeruginosa to antibiotics, it is crucial to develop new therapeutic options for infections caused by this bacterium. Bacterial metabolic pathways are being explored as potential targets for new antibiotics. This study investigates the metabolic mechanisms of glycerol and its intermediate glycerol 3-phosphate (G3P) in P. aeruginosa PAO1. The results reveal the importance of G3P homeostasis for the growth and virulence factor production of P. aeruginosa PAO1. The enzyme GlpD, involved in G3P catabolism, is identified as a potential therapeutic target for the prevention and treatment of P. aeruginosa infections.
Article
Biochemistry & Molecular Biology
Kevin Wang, Jonathan Hirschenson, Amanda Moore, Ryan J. Mailloux
Summary: This study found that the glutathionylation of mitochondrial complex I can lead to increased production of ROS during reverse electron transfer from the ubiquinone pool. This has implications for understanding the relationship between mitochondrial glutathionylation reactions and oxidative stress induction in various pathologies.
Article
Biochemistry & Molecular Biology
Clauber Henrique Souza da Costa, Ted Wilson Bichara, Guelber Cardoso Gomes, Alberto Monteiro dos Santos, Kaue Santana da Costa, Anderson Henrique Lima e Lima, Claudio Nahum Alves, Jeronimo Lameira
Summary: Allosteric changes in enzyme activity are modulated by ligand binding, with implications for drug design. This study focused on analyzing the conformational dynamics of glycerol-3-phosphate dehydrogenase in Leishmania mexicana, providing insights into potential drug discovery targets in the reversible redox conversion pathway.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Cell Biology
Zilv Luo, Zhaowei Chen, Zijing Zhu, Yiqun Hao, Jun Feng, Qiang Luo, Zongwei Zhang, Xueyan Yang, Jijia Hu, Wei Liang, Guohua Ding
Summary: The study shows that Ang II upregulates GPD1 expression and promotes G-3-P synthesis in podocytes, leading to lipid accumulation and mitochondrial dysfunction. Knockdown of GPD1 protects podocytes, while overexpression of GPD1 exacerbates Ang II-induced cell injury.
CELLULAR SIGNALLING
(2022)
Article
Agricultural Engineering
Pseudomonas Asiatica, Trinh Thi Nguyen, Suman Lama, Satish Kumar Ainala, Mugesh Sankaranarayanan, Ashish Singh Chauhan, Jung Rae Kim, Sunghoon Park
Summary: The study demonstrates that Pseudomonas asiatica C1 can be a promising host for the production of 3-HP from glycerol by genetic editing and optimization, achieving high production efficiency and molar yield on glucose.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Jueun Lee, Jin A. Shin, Eun-Mi Lee, Miso Nam, Eun-Mi Park
Summary: Recent studies demonstrate that changes in energy metabolism in activated microglia play a role in their immune responses in the ischemic brain. Noggin, an antagonist of bone morphogenetic protein, has been found to enhance myelination in the ischemic brain, and conditioned media (CM) from activated BV2 microglia treated with noggin can increase the expression of myelin basic protein (MBP) in oligodendrocytes. Metabolite analysis revealed that noggin treatment suppressed the increase in intracellular glucose and lactate levels in BV2 cells after ischemia/reperfusion (I/R), but increased extracellular levels of glucose and several amino acids. In MO3.13 cells, noggin CM reversed changes in metabolite levels induced by vehicle CM, suggesting that noggin-induced metabolic changes in microglia create a favorable environment for myelin synthesis in oligodendrocytes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Yilin Liu, Xiaomin Huo, Shanshan Yu, Wenhao Dong, Yongchang Xue, Changbin Liu
Summary: In this study, researchers expressed the transcription factor LEAFY COTYLEDON 2 from Arabidopsis thaliana in Chlorella sorokiniana and found that it significantly improved the relative electron transport rate under high-intensity light, leading to increased lipid content and altered fatty acid composition. Through analysis of differentially expressed proteins, the study revealed the regulatory mechanisms of AtLEC2 on TAG biosynthesis and photosynthesis-related proteins, facilitating fixed carbon flow in photosynthesis to TAG.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Genetics & Heredity
Madhulika Rai, Sarah M. Carter, Shefali A. Shefali, Nader H. Mahmoudzadeh, Robert Pepin, Jason M. Tennessen
Summary: The enzyme glycerol-3-phosphate dehydrogenase 1 (GPDH1) plays essential roles in the development and metabolism of Drosophila melanogaster. Depletion of maternal and zygotic pools of GPDH1 affects larval development, lifespan, fat storage, oogenesis, and embryogenesis. Metabolomics analysis further reveals metabolic adaptations in GPDH1 mutants that differ between generations. These findings expand our understanding of GPDH1's functions in early development and provide insights into the survival mechanisms of flies lacking this key enzyme.
G3-GENES GENOMES GENETICS
(2022)
Article
Food Science & Technology
Zhi-Xiang Liu, Lin-Lin Yuan, Nan Zhang, Min Zhao, Yue Li, Ming-Yue Zhao, Yu-Meng Wang, Zi-Zhuo Wang, Yang-Yang Zhang, Jin-Long Tian
Summary: In this study, phenylpropanoids in N. tangutorum were rapidly and efficiently discovered by UPLC-MS guided separation. Fifteen phenylpropanoids were identified, and they were found to be the chemical basis for the hypoglycemic effects of N. tangutorum. Glycerol phosphate dehydrogenase (GPDH) was identified as the most likely target of the compounds.
Article
Agriculture, Multidisciplinary
Minyoung Kim, Jongchan Lee, Lynn Heo, Sang Jun Lee, Sang-Wook Han
Summary: Acidovorax citrulli (Ac) is the causal agent of bacterial fruit blotch in watermelon. A putative glycerol-3-phosphate dehydrogenase in Ac (GlpdAc) was found to play a role in glycerol metabolism, glycolysis, cell aggregation, and virulence. This study expands the understanding of proteins associated with virulence in Ac.
PLANT PATHOLOGY JOURNAL
(2021)
Review
Cell Biology
Matthew J. Merrins, Barbara E. Corkey, Richard G. Kibbey, Marc Prentki
Summary: This review focuses on recent developments in the understanding of nutrient-induced insulin secretion. It challenges the canonical model of ATP production and K-ATP channels. The study discusses the importance of beta cell metabolic oscillations, the alignment of metabolic cycles with insulin secretion, and the regulatory roles of metabolic signaling components. A refined model of the signaling process is proposed for evaluation by the field.
Article
Multidisciplinary Sciences
Zhengchao Xia, Ningming Zhao, Mingzhou Liu, DanDan Jiang, Shanjun Gao, Peizhi Ma, Li Huang
Summary: This study found that GPD1 acts as a tumor suppressor in breast cancer by inhibiting proliferation, migration, and invasion of cancer cells through activating lipid synthesis and the PI3K/AKT signaling pathway. Additionally, a positive correlation was observed between GPD1 level and survival rate in breast cancer patients. Therefore, GPD1 could serve as a prognostic biomarker and potential therapeutic target for breast cancer.
Article
Multidisciplinary Sciences
Morteza Ghazanfari Jajin, Raziyeh Abooshahab, Kourosh Hooshmand, Ali Moradi, Seyed Davar Siadat, Roghieh Mirzazadeh, Koorosh Goodarzvand Chegini, Mehdi Hedayati
Summary: In this study, plasma metabolomics profiling was performed to explore the metabolic pathways involved in the tumorigenesis of medullary thyroid cancer (MTC). The results revealed perturbations in the biosynthesis of unsaturated fatty acids and amino acid metabolisms, particularly leucine, glutamine, and glutamate, which are tightly responsible for tumor cells' energy production. Furthermore, specific metabolites such as linoleic acid, linolenic acid, and leucine showed high discriminatory power in distinguishing MTC patients from healthy individuals. This research provides insights into the metabolic profile of MTC and highlights the potential of metabolomics as a diagnostic tool.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Ankit Dhoundiyal, Vanessa Goeschl, Stefan Boehm, Helmut Kubista, Matej Hotka
Summary: Electrical activity in neurons is accompanied by an increase in cytosolic Ca2+ concentration, which is crucial for securing energy supply. The glycerol-3-phosphate shuttle (G3PS) plays a significant role in neuronal energy supply when other energy transfer pathways are not available.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ines Baptista, Effrosyni Karakitsou, Jean-Baptiste Cazier, Ulrich L. Guenther, Silvia Marin, Marta Cascante
Summary: Through TKTL1 knockdown experiments, it was found that TKTL1 plays a key role in the metabolic adaptation to hypoxia in THP-1 AML cells by modulating the activities of G6PD and GAPDH, which involve changes in redox capacity and transcriptional regulation of key enzymes and transporters related to glucose, PPP, and amino acid metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dermatology
Jiaxing You, Chao Liu, Yixin Chen, Weifen Zhu, Hongye Li, Lin Li
Summary: The aim of this study was to identify the most cited articles in the field of diabetic foot ulcer (DFU) research. The study conducted a bibliometric analysis using Boolean search terms in two databases. The findings revealed the characteristics of the top-cited articles, such as publication year, country of origin, and level of evidence. The majority of the articles were clinical studies, providing valuable insights into the history and development of DFU research.
INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS
(2023)
Article
Green & Sustainable Science & Technology
Xiang Wang, Si-Fen Liu, Zhen-Yao Wang, Yao Wang, Ting-Bin Hao, Srinivasan Balamurugan, Da-Wei Li, Yuhe He, Hong-Ye Li, Carol Sze Ki Lin
Summary: The research on sustainable alternatives for producing valuable polyunsaturated fatty acids (PUFAs) has been promoted due to the limited supply of wild deep-sea fish and the potential accumulation of toxic pollutants. In this study, an evolved strain of Phaeodactylum tricornutum microalgae was generated through adaptive evolution, resulting in enhanced biomass and PUFA production capabilities. The use of food waste hydrolysate as a carbon source further improved biomass yield and PUFA production. This study provides novel insights into sustainable microalgal-based waste valorisation for PUFA production.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Microbiology
Yunpeng Gai, Lei Li, Bing Liu, Haijie Ma, Yanan Chen, Fang Zheng, Xuepeng Sun, Mingshuang Wang, Chen Jiao, Hongye Li
Summary: This study identifies the crucial role of bZIP transcription factors in fungal morphogenesis, abiotic stress response, and pathogenesis in phytopathogenic fungus Alternaria alternata. The findings expand our understanding of how microbial pathogens utilize these genes to cope with environmental stresses and achieve successful infection in the host plant.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Chemistry, Medicinal
Yang Liu, Siyuan Xu, Qiudie Cai, Dawei Li, Hongye Li, Weidong Yang
Summary: In this study, we investigated the interaction between okadaic acid (OA) and gut microbiota using an in vitro fermentation experiment. We found that OA can reshape the bacterial composition and lead to the production of various metabolites. Our findings confirmed the direct interaction between OA and gut bacteria, providing valuable evidence for understanding the metabolic process and complex toxicity of OA.
Article
Engineering, Environmental
Yuhan Ma, Wasiqi Yuan, Wenjie Kan, Changyue Huang, Jiawei Zhu, Guilong Zhang, Hongye Li, Srinivasan Balamurugan, Lifang Wu
Summary: Microalgae are being investigated as green cell factories for biochemistry and pollution control. This study focuses on enhancing the synthesis of selenium nanoparticles (SeNPs) in microalgae and understanding the molecular mechanisms involved. Chlorella vulgaris was fortified with quercetin to promote SeNPs biotransformation. Quercetin facilitated the formation of SeNPs within 72 hours, converting almost 97% of inorganic selenite to SeNPs. Electron microscopy confirmed the biogenic ability of the algal system. Quercetin fortification protected C. vulgaris from selenite-induced impairment and improved its growth rate, while also altering the fatty acid profile.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Li Li, Shuang Chen, Si-yuan Xu, Da-wei Li, Wei-dong Yang
Summary: This study investigated the toxicity of Gambierdiscus caribaeus on marine medaka and found that direct or indirect exposure to G. caribaeus through the food chain can affect the behavior of marine medaka and cause pathological damage to its tissues. Transcriptome sequencing and RT-qPCR analysis revealed that G. caribaeus exposure triggers physiological and biochemical responses in marine medaka.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Agronomy
Bei Liu, Xingxing Liang, Jinchao Kong, Chen Jiao, Hongye Li, Yunpeng Gai
Summary: In this study, the genetic structure and diversity of Colletotrichum gloeosporioides, the dominant species causing citrus anthracnose, were analyzed using the Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. A total of 19 GAPDH haplotypes were identified, with the main haplotype (haplotype 5) distributed widely among isolates from different Citrus species and locations. The genetic differentiation index (Fst) revealed significant genetic differentiation between populations from different regions and host species. The results suggest that genetic differentiation in C. gloeosporioides is mainly influenced by gene mutation, recombination, or migration within native populations rather than natural selection caused by geography or host variety.
Article
Agriculture, Multidisciplinary
Li-Gong Zou, Yi-Tong Yao, Fu-Fang Wen, Xiao Zhang, Bo-Tao Liu, Da-Wei Li, Yu-Feng Yang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: The excessive accumulation of catechol in the environment due to its burgeoning commercial applications poses a severe ecological threat. This study investigated the potential of the microalga Crypthecodinium cohnii to degrade catechol and utilize the byproduct as a carbon source. Results showed that catechol significantly increased the growth of C. cohnii and was rapidly catabolized within 60 hours. Transcriptomic analysis identified key genes involved in catechol degradation, and real-time polymerase chain reaction (RT-PCR) analysis revealed a remarkable increase in the transcription of these genes. The study also demonstrated altered primary metabolite content and the ability of C. cohnii to tolerate catechol treatment without adverse effects.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Zi-Hao Qin, Xiaomeng Hu, Jin-Hua Mou, Guo-Hui He, Guang-Bin Ye, Hong-Ye Li, Shauhrat Singh Chopra, Liang Dong, Carol Sze Ki Lin, Xiang Wang
Summary: This study conducted Life Cycle Assessments (LCA) to evaluate the environmental impact of laboratory-scale EPA production by Phaeodactylum tricornutum. The results showed that technical advancements, particularly metabolic engineering, significantly reduced the global warming potential and cumulative energy demand associated with EPA production. Electricity consumption and extraction solvents were identified as the main contributors to the environmental impact. The study suggests future directions should focus on increasing EPA productivity, adopting alternative cultivation approaches with less energy input, and implementing environmentally friendly methods for EPA recovery.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Chemistry, Medicinal
Kuan-Kuan Yuan, Zi-Min Chen, Ya-Xin Liu, Hong-Ye Li, Wei-Dong Yang
Summary: This study found that docosahexaenoic acid (DHA) can enhance the response of Perna viridis to diarrhetic shellfish toxins (DSTs) produced by Prorocentrum lima. The addition of DHA increased the esterification level of DSTs, activated the Nrf2 signaling pathway, and alleviated the damage to the digestive glands. These findings provide insights into the role of DHA in the environmental adaptation of bivalves.
Article
Agronomy
Yunpeng Gai, Qichen Niu, Jinchao Kong, Lei Li, Xingxing Liang, Yuwei Cao, Xianqi Zhou, Xuepeng Sun, Haijie Ma, Mingshuang Wang, Neeraj Shrivastava, Hongye Li, Chen Jiao
Summary: A comparative transcriptome analysis was performed to study the gene expression patterns of Alternaria alternata during infection of citrus. Significant gene expression differences were found between the early and late stages of infection. Several novel genes related to plant-pathogen interaction were identified. RT-qPCR analysis confirmed the expression patterns of selected genes from the peroxisome pathway.
Article
Microbiology
Wen Wang, Tao Xiong, Yating Zeng, Wenwen Li, Chen Jiao, Jianping Xu, Hongye Li
Summary: This study conducted an extensive survey of Phyllosticta species across 11 citrus-producing provinces in southern China and identified five Phyllosticta species with pathogenicity on different citrus species. The findings have important implications for the control and management of Citrus Black Spot and related diseases.
Article
Engineering, Environmental
Jin-Hua Mou, Zi-Hao Qin, Yu-Feng Yang, Si-Fen Liu, Wei Yan, Lan Zheng, Ya-Hui Miao, Hong-Ye Li, Patrick Fickers, Carol Sze Ki Lin, Xiang Wang
Summary: This study investigated the characteristics of four types of food waste and their hydrolysates, and explored their potential application in biorefineries. The results showed that the characteristics of food waste and their hydrolysates varied based on their origins, and the origin and storage conditions of food waste affected microalgal growth and paramylon production.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Marine & Freshwater Biology
Jian-Wei Zheng, Guo-Hui He, Rui-Xia Xu, Xiang Wang, Hong-Ye Li, Wei-Dong Yang
Summary: Studies have found that there are reciprocal inhibitory effects between Karenia mikimotoi and Prorocentrum shikokuense under co-cultures. RNA sequencing analysis revealed that genes involved in photosynthesis, carbon fixation, energy metabolism, nutrients absorption and assimilation were up-regulated in K. mikimotoi after co-cultured with P. shikokuense, while genes involved in DNA replication and cell cycle were down-regulated. In contrast, genes involved in energy metabolism, cell cycle, and nutrients uptake and assimilation were down-regulated in P. shikokuense under co-culture with K. mikimotoi. Additionally, the expression of PLA2G12 and nitrate reductase, which may play important roles in the allelopathy of K. mikimotoi, was significantly increased. These findings provide new insights into the interspecies competition between K. mikimotoi and P. shikokuense and offer a novel strategy for studying interspecific competition in complex systems.
Article
Public, Environmental & Occupational Health
Jinping Zhang, Peng Liu, Morong Wang, Jie Wang, Jie Chen, Wenling Yuan, Mei Li, Zhijuan Xie, Wangping Dong, Hongye Li, Yan Zhao, Lun Wan, Tian Chu, Lu Wang, Hui Zhang, Ting Tao, Jing Ma
Summary: This study analyzed the clinical features and diagnostic methods of 19 patients with COVID-19. The findings showed that there were more male than female critically ill patients, and they often had symptoms such as fever and dry cough. Chest computed tomography revealed ground-glass changes in all cases. Significant changes were observed in clinical laboratory indices, especially in the D-dimer level of non-survivors.
JOURNAL OF PUBLIC HEALTH-HEIDELBERG
(2022)
