Article
Energy & Fuels
Lucia Coimbra, Karen Malan, Alejandra Fagundez, Mairan Guigou, Claudia Lareo, Belen Fernandez, Martin Pratto, Silvia Batista
Summary: Genetically engineered yeast strains were developed for efficient ethanol production from lignocellulosic biomass. The recombinant strain CAT-1-XIT (pRS42K::XI) showed promising results with 74% consumption of D-xylose and production of 12.6 g/L ethanol.
BIOENERGY RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Jung-Hoon Bae, Mi-Jin Kim, Bong Hyun Sung, Yong-Su Jin, Jung-Hoon Sohn
Summary: The extracellular conversion of xylose to xylulose before uptake has been found to be effective in xylose fermentation or glucose/xylose co-fermentation, reducing the competition between glucose and xylose for cellular uptake. The engineered XI secretion system developed in this study may enable the simultaneous utilization of C5/C6 sugars from sustainable lignocellulosic biomass.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Meilin Kong, Xiaowei Li, Tongtong Li, Xuebing Zhao, Mingjie Jin, Xin Zhou, Hanqi Gu, Vladimir Mrsa, Wei Xiao, Limin Cao
Summary: CCW12(OE) is a Saccharomyces cerevisiae strain constructed by overexpressing CCW12, which shows high ethanol production efficiency in various hydrolysates and exhibits better stress resistance and fermentation performance compared to the control strain WXY70.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agronomy
Heeyoung Park, Sang Un Park, Byeong-Kwan Jang, Jeong Jae Lee, Yong Suk Chung
Summary: Kenaf, an annual fiber crop mainly grown in India and China, is emerging as a promising bio-based energy source due to its rapid growth, CO2 absorption capabilities, and high productivity. The study evaluated 10 different cultivars of kenaf for cellulosic ethanol production, finding that ethanol yield and productivity were significantly impacted by cultivar type, rather than biomass composition. Engineering yeast to utilize xylose under pH control can maximize ethanol production from kenaf.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Review
Biotechnology & Applied Microbiology
Dielle Pierotti Procopio, Emanuele Kendrick, Rosana Goldbeck, Andre Ricardo de Lima Damasio, Telma Teixeira Franco, David J. Leak, Yong-Su Jin, Thiago Olitta Basso
Summary: The engineering of xylo-oligosaccharide-consuming Saccharomyces cerevisiae strains is a promising approach for the effective utilization of lignocellulosic biomass and development of economic industrial fermentation processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Gabriel de Souza Colombo, Isis Viana Mendes, Betulia de Morais Souto, Cristine Chaves Barreto, Luana Assis Serra, Eliane Ferreira Noronha, Nadia Skorupa Parachin, Joao Ricardo Moreira de Almeida, Betania Ferraz Quirino
Summary: The current climate crisis calls for the replacement of fossil energy sources with sustainable alternatives. This study presents a new bacterial XI enzyme that has been successfully expressed in Saccharomyces cerevisiae, allowing efficient consumption and metabolism of xylose.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Haijie Wang, Limin Cao, Qi Li, Nalin N. Wijayawardene, Jian Zhao, Min Cheng, Qi-Rui Li, Xiaobin Li, Itthayakorn Promputtha, Ying-Qian Kang
Summary: Efficient renewable bioethanol can alleviate energy crisis and environmental pollution. Genetically modified strain GRE3(OE) shows remarkable fermentation efficiency in different lignocellulosic hydrolysates, making it a promising candidate for industrial ethanol development.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agricultural Engineering
Xiaowei Li, Rui Zhao, Shan Li, Yating Wang, Xinnan Wang, Wenya Yang, Mengchan Yang, Weihua Xiao, Shihui Yang, Xiaoqing Lin, Xiaojie Zheng, Xiaojun Ma, Ling Zhao, Wei Xiao, Limin Cao
Summary: This study focused on improving yeast strains for mixed sugar fermentation in lignocellulosic hydrolysates, with SPT2(OE) showing superior ethanol yield and titer within 24 hours in different hydrolysates.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Biotechnology & Applied Microbiology
Juhi Sharma, Vinod Kumar, Rajendra Prasad, Naseem A. Gaur
Summary: Lignocellulosic biomass is an abundant material on earth and can be processed by microorganisms to produce biofuels. Saccharomyces cerevisiae is an ideal candidate for consolidated bio-processing (CBP) to produce cellulose-based ethanol. However, there are still challenges in the process, such as incomplete substrate catabolism and low expression of heterologous proteins.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Celina Borgstrom, Viktor C. Persson, Oksana Rogova, Karen O. Osiro, Ester Lundberg, Peter Spegel, Marie Gorwa-Grauslund
Summary: In this study, using GFP-based biosensors, the researchers found that the sensing response to D-xylose is similar to that of low concentrations of D-glucose. They hypothesized that the formation of glycolytic intermediates may be the cause of this response. Further analysis confirmed systemic changes in intracellular sugar phosphate levels after disrupting glycolysis by deleting the PGI1 gene. The results suggest that targeting the metabolic node around Pgi1p and the level of D-fructose-6-phosphate could potentially improve D-xylose utilization.
MICROBIAL CELL FACTORIES
(2022)
Article
Food Science & Technology
Peizhou Yang, Jianchao Chen, Wenjing Wu, Suwei Jiang, Yanhong Deng, Jiuling Lu, Hu Wang, Yong Zhou, Yuyou Geng, Zhi Zheng
Summary: CRISPR-Cas9 was used to knock out MET5 and SIZ1 genes in S. cerevisiae MET5 and SIZ1 using XYL1 and XYL2 expression cassettes, leading to improved tolerance to organic acids and increased xylose utilization efficiency. The co-deletion of MET5 and SIZ1 resulted in enhanced tolerance to acetic acid and lactic acid. Transcriptomics analysis showed up-regulation of carbon metabolism and down-regulation of ribosome biogenesis in S. cerevisiae MET5dSIZ1d. This mutant strain exhibited higher ethanol concentrations in both kiwifruit and corn stalk hydrolysate fermentations, indicating its potential for ethanol production from cellulosic hydrolysates and substrates rich in organic acids.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yang Sun, Meilin Kong, Xiaowei Li, Qi Li, Qian Xue, Junyan Hou, Zefang Jia, Zhipeng Lei, Wei Xiao, Shuobo Shi, Limin Cao
Summary: A yeast strain suitable for 2G bioethanol production was successfully developed using metabolic engineering and adaptive evolution. The strain exhibited improved xylose utilization, higher tolerance against inhibitors, reduced glycerol by-product production, and outperformed in ethanol production using industrial hydrolysate.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agronomy
Fangqing Wei, Menglei Li, Ming Wang, Hongxing Li, Zailu Li, Wensheng Qin, Tiandi Wei, Jianzhi Zhao, Xiaoming Bao
Summary: The study engineered LF1 yeast strain to improve robustness through ARTP mutagenesis, resulting in mutant LF1-6 with enhanced tolerance but reduced xylose utilization. Iterative ARTP mutagenesis produced strain 6M-15, restoring xylose utilization and exhibiting high co-fermentation performance for ethanol production.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Efri Mardawati, Emilda Ayu Febrianti, Hana Nur Fitriana, Tri Yuliana, Norisca Aliza Putriana, Sri Suhartini, Kasbawati
Summary: This study investigated the potential of using oil palm empty fruit bunch (OPEFB) as a biomass waste for xylitol and ethanol production. Integrated microaerobic xylitol production and anaerobic ethanol semi simultaneous saccharification and fermentation methods were used. The results showed that OPEFB with high cellulose and hemicellulose content can be a valuable resource for sustainable production of biofuels and biochemicals.
Article
Biotechnology & Applied Microbiology
Patricia J. Slininger, Maureen A. Shea-Andersh, Stephanie R. Thompson, Bruce S. Dien, Cletus P. Kurtzman, Venkatesh Balan, Leonardo da Costa Sousa, Nirmal Uppugundla, Bruce E. Dale, Michael A. Cotta
BIOTECHNOLOGY FOR BIOFUELS
(2015)
Article
Biochemistry & Molecular Biology
Michael J. Bowman, Bruce S. Dien, Karl E. Vermillion, Jeffrey A. Mertens
CARBOHYDRATE RESEARCH
(2015)
Article
Chemistry, Applied
Divya Ramchandran, Ping Wang, Bruce Dien, Wei Liu, Michael A. Cotta, Vijay Singh
Article
Agronomy
Michael D. Casler, Jerome H. Cherney, E. Charles Brummer, Bruce S. Dien
Article
Agriculture, Multidisciplinary
Ming-Hsu Chen, Kelly S. Swanson, George C. Fahey, Bruce S. Dien, Alison N. Beloshapka, Laura L. Bauer, Kent D. Rausch, M. E. Tumbleson, Vijay Singh
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2016)
Article
Environmental Sciences
Akwasi A. Boateng, Michelle J. Serapiglia, Charles A. Mullen, Bruce S. Dien, Fawzy M. Hashem, Robert B. Dadson
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2015)
Review
Biotechnology & Applied Microbiology
Mingjie Jin, Patricia J. Slininger, Bruce S. Dien, Suresh Waghmode, Bryan R. Moser, Andrea Orjuela, Leonardo da Costa Sousal, Venkatesh Balan
TRENDS IN BIOTECHNOLOGY
(2015)
Article
Chemistry, Multidisciplinary
Ya-Ping Xue, Mingjie Jin, Andrea Orjuela, Patricia J. Slininger, Bruce S. Dien, Bruce E. Dale, Venkatesh Balan
Article
Energy & Fuels
N. Qureshi, S. Liu, S. Hughes, D. Palmquist, B. Dien, B. Saha
BIOENERGY RESEARCH
(2016)
Article
Agricultural Engineering
Sun Min Kim, Bruce S. Dien, M. E. Tumbleson, Kent D. Rausch, Vijay Singh
BIORESOURCE TECHNOLOGY
(2016)
Correction
Biotechnology & Applied Microbiology
Patricia J. Slininger
BIOTECHNOLOGY AND BIOENGINEERING
(2016)
Article
Biotechnology & Applied Microbiology
Patricia J. Slininger, Bruce S. Dien, Cletus P. Kurtzman, Bryan R. Moser, Erica L. Bakota, Stephanie R. Thompson, Patricia J. O'Bryan, Michael A. Cotta, Venkatesh Balan, Mingjie Jin, Leonardo da Costa Sousa, Bruce E. Dale
BIOTECHNOLOGY AND BIOENGINEERING
(2016)
Article
Chemistry, Applied
Ming-Hsu Chen, Michael J. Bowman, Michael A. Cotta, Bruce S. Dien, Loren B. Iten, Terence R. Whitehead, Kent D. Rausch, M. E. Tumbleson, Vijay Singh
CARBOHYDRATE POLYMERS
(2016)
Article
Plant Sciences
Erin D. Scully, Tammy Gries, Gautam Sarath, Nathan A. Palmer, Lisa Baird, Michelle J. Serapiglia, Bruce S. Dien, Akwasi A. Boateng, Zhengxiang Ge, Deanna L. Funnell-Harris, Paul Twigg, Thomas E. Clemente, Scott E. Sattler
Article
Chemistry, Multidisciplinary
Bruce S. Dien, J. Y. Zhu, Patricia J. Slininger, Cletus P. Kurtzman, Bryan R. Moser, Patricia J. O'Bryan, Roland Gleisner, Michael A. Cotta