Article
Biotechnology & Applied Microbiology
Aafke C. A. van Aalst, Sophie C. de Valk, Walter M. van Gulik, Mickel L. A. Jansen, Jack T. Pronk, Robert Mans
Summary: This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of Saccharomyces cerevisiae. It discusses the context dependency, theoretical impact, and potential for industrial application of different strategies.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Review
Chemistry, Physical
Christiaan H. L. Tempelman, Ryan Oozeerally, Volkan Degirmenci
Summary: Lignocellulosic biomass is a cheap and plentiful resource that could play a key role in sustainable chemical production. The conversion of simple sugars in lignocellulosic biomass into commercially valuable products like 5-hydroxymethyl furfural (HMF) is a significant challenge and various heterogeneous catalysts have been proposed to address this issue.
Article
Chemistry, Multidisciplinary
Navya Subray Bhat, Shobhita L. Hegde, Saikat Dutta, Putla Sudarsanam
Summary: This study presents an efficient method to synthesize HMF esters from biomass-derived CMF, with potential applications as chemical intermediates, fuel additives, and bioactive compounds. By converting microcrystalline cellulose into CMF under optimized conditions and further transforming it into HMF esters, high yields were achieved.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Physical
Rui Li, Qixuan Lin, Yao Liu, Xiaohui Wang, Chuanfu Liu, Feng Peng, Junli Ren
Summary: The solvent effect on the selective production of furfural or 5-hydroxymethylfurfural (HMF) from fructose was investigated using experiments, nuclear magnetic resonance (NMR), and molecular dynamics (MD) simulation. It was found that solvent polarity and basicity played a crucial role in the selective formation of furfural and HMF. The arrangement of solvents around fructose tautomers, particularly around different C-OH, resulted in different products. High yields of furfural (76.2%) and HMF (90.5%) were achieved in γ-butyrolactone (GBL)-H2O and dimethyl sulfoxide (DMSO)-H2O solvents.
JOURNAL OF CATALYSIS
(2023)
Article
Chemistry, Inorganic & Nuclear
Shaofeng Xiong, Xiaona Liu, Zhigang Shen, Zhongting Hu, Hongyun Yang, Jian Hao, Jinjun Cai, Jie Yang
Summary: A sulfur vacancy-rich Zn3In2S6/Bi2MoO6 heterojunction photocatalyst was developed for the selective oxidation of biomass-derived molecules and the production of H-2. The catalyst exhibited outstanding performance and could contribute to the development of new technologies for biomass resource utilization.
INORGANIC CHEMISTRY
(2023)
Article
Engineering, Environmental
Pierre Desir, Dionisios G. Vlachos
Summary: This study investigates fructose dehydration to HMF in a biphasic microreactor with high yields and optimal organic-to-water ratios identified at high temperatures. The research also uncovers the dual role of the solvent in both protecting HMF from degradation and modifying fundamental chemistry. Additionally, mass transfer limitations in microreactors at longer residence times and higher temperatures are revealed, highlighting the importance of reactor design and operation.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Daniele Polidoro, Alvise Perosa, Maurizio Selva
Summary: In this study, three different multiphase systems were investigated for the oxidation of 5-hydroxymethyl-furfural (HMF). The conditions for each system allowed for excellent control of the reaction selectivity and high yields of the desired products. The catalyst was efficiently segregated and recycled under all tested conditions.
Article
Agricultural Engineering
Tingwei Zhang, Wenzhi Li, Huining Xiao, Yongcan Jin, Shufang Wu
Summary: This article summarizes the advantages and disadvantages of different catalytic systems for the synthesis of furfural from biomass and biomass pretreatment hydrolysate based on recent literature, and puts forward suggestions for furfural production research.
BIORESOURCE TECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Olja Simoska, Zayn Rhodes, Samali Weliwatte, Jaime R. Cabrera-Pardo, Erin M. Gaffney, Koun Lim, Shelley D. Minteer
Summary: The development of electrochemical catalytic conversion of 5-hydroxymethylfurfural (HMF) has gained attention recently, but is still in its early stages with limited practical applications due to low catalytic activity and product selectivity. Understanding catalytic processes and designing electrocatalysts are crucial for achieving selective and complete conversion.
Article
Agricultural Engineering
Jorge Carlos Avila-Gaxiola, Evangelina Avila-Gaxiola
Summary: This study evaluates the feasibility of alcoholic fermentation using S. cerevisiae yeast in enzymatically treated Agave tequilana leaves, and the results demonstrate the potential and efficiency of ethanol production, with applications in industry and biotechnology.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Green & Sustainable Science & Technology
Mattia Annatelli, Giacomo Trapasso, Davide Dalla Torre, Luca Pietrobon, Davide Redolfi-Bristol, Fabio Arico
Summary: In this study, a green synthetic approach to 5,5'-(oxybis(methylene)]bis-2-furfural (OBMF) from 5-hydroxymethyl-2-furfural (HMF) is reported. The reaction conditions were optimized, using dimethyl carbonate (DMC) as the solvent and iron(III) sulfate as the catalyst. OBMF and its derivatives showed high yields and thermal stability, making them valuable bio-based monomers for bioplastics preparation.
ADVANCED SUSTAINABLE SYSTEMS
(2022)
Article
Chemistry, Physical
Ryan Oozeerally, David L. Burnett, Thomas W. Chamberlain, Reza J. Kashtiban, Steven Huband, Richard Walton, Volkan Degirmenci
Summary: Metal organic framework UiO-66 was studied as a heterogeneous catalyst for glucose conversion, with modified UiO-66 showing higher isomerisation activity. Replacing 50% of the linkers with sulfonate-containing linkers resulted in increased isomerisation activity, while the introduction of naphthalene-dicarboxylate linkers improved fructose selectivity.
Article
Chemistry, Applied
Xiaoyun Li, Xuebin Lu, Wenxuan Hu, Haocheng Xu, Jingguang Chen, Jian Xiong, Lefu Lu, Zhihao Yu, Chuanling Si
Summary: In this study, phosphotungstic acid functionalized biochar (PA-FCB) was synthesized in one step and used for the conversion of corncob to furfural. The results showed that PA-FCB exhibited excellent catalytic performance and high furfural yield.
FUEL PROCESSING TECHNOLOGY
(2022)
Article
Materials Science, Paper & Wood
Ting Huang, Yaohong Zhou, Xiaohua Zhang, Dayong Peng, Xuliang Nie, Jing Chen, WanMing Xiong
Summary: A furfuryl alcohol resin-based solid acid catalyst (SC-FAR-800) was prepared and applied in the conversion of carbohydrates into furan compounds. The catalyst exhibited good catalytic activity and stability, achieving high yields of furfural and 5-HMF from various carbohydrates. It also showed excellent reusability and could be regenerated for further use.
Article
Agricultural Engineering
Tao Yang, Wenzhi Li, Ajibola T. Ogunbiyi, Shengxin An
Summary: A novel solid acid catalyst SO3H-NG-C was synthesized from biomass-derived glucosamine hydrochloride and used for converting corn stover into furfural and 5-HMF. Through systematic investigation, optimal reaction conditions were identified to achieve high yields, and the catalyst demonstrated reasonable stability for corn stover conversion.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Stefano Bertacchi, Maurizio Bettiga, Danilo Porro, Paola Branduardi
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Multidisciplinary Sciences
Veronica Saez-Jimenez, Zeljka Sanader Marsic, Matteo Lambrughi, Jae Ho Shin, Robin van Havere, Elena Papaleo, Lisbeth Olsson, Valeria Mapelli
Article
Biochemistry & Molecular Biology
Matteo Lambrughi, Zeljka Sanader Marsic, Veronica Saez-Jimenez, Valeria Mapelli, Lisbeth Olsson, Elena Papaleo
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)
Article
Biochemistry & Molecular Biology
Nicoletta Guaragnella, Maurizio Bettiga
Summary: Acetic acid stress is a common challenge for yeast cells in various environmental conditions and industrial bioprocesses, and its response involves cellular transport, pH homeostasis, metabolism, and stress-signalling pathways.
Article
Biotechnology & Applied Microbiology
Mohsen Karimi, Tove Widen, Yvonne Nygard, Lisbeth Olsson, Henrik Strom
Summary: This study presents a validated Computational Fluid Dynamics (CFD) model for mass transfer in bioelectrochemical systems, demonstrating its capability in simulating hydrodynamics with confidence. The simplified bubble size distribution model improves accuracy in mass transfer predictions, leading to significant enhancements when compared against experimental data. Additionally, the modeling of different stirring rates in microbial cultivation processes highlights the importance of optimizing mass transfer for gas fermentation and microbial electrosynthesis.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Monika Tolgo, Silvia Huttner, Peter Rugbjerg, Nguyen Thanh Thuy, Vu Nguyen Thanh, Johan Larsbrink, Lisbeth Olsson
Summary: The study sequenced and analyzed the genome and transcriptome of the thermophilic Ascomycete Thielavia terrestris strain LPH172, revealing a rich arsenal of CAZymes and auxiliary activity (AA) domains. Upregulation of different encoding genes on different substrates suggests a complex and nuanced degradation strategy employed by the fungus.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Microbiology
Marie-Anne Deprez, Jeroen M. Maertens, Lisbeth Olsson, Maurizio Bettiga, Joris Winderickx
Summary: Acetic acid influences the growth and lifespan of yeast cells, with mutants lacking V-ATPase being more susceptible to growth arrest in high acetic acid concentrations. Supplementation of 20 mM acetic acid extends the lifespan of all tested strains, including the V-ATPase compromised mutants.
Article
Biotechnology & Applied Microbiology
Monika Tolgo, Olav A. Hegnar, Heidi Ostby, Aniko Varnai, Francisco Vilaplana, Vincent G. H. Eijsink, Lisbeth Olsson
Summary: This study provides a detailed functional characterization of six AA9 LPMOs from Thermothielavioides terrestris LPH172. The research shows that these LPMOs have different activities on various cellulose substrates, further supporting the hypothesis that the multiplicity of LPMO genes is related to the complex structure of lignocellulosic biomass.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jenny Arnling Baath, Vera Novy, Leonor Carneiro, Georg M. Guebitz, Lisbeth Olsson, Peter Westh, Doris Ribitsch
Summary: This study rigorously characterized two almost identical cutinases and demonstrated that even minor sequence differences in cutinases can drastically affect their substrate binding, substrate specificity, and catalytic efficiency.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Marlous van Dijk, Peter Rugbjerg, Yvonne Nygard, Lisbeth Olsson
Summary: Using RNA sequencing, this study identified differential gene expression in industrial yeast during short-term adaptation to lignocellulosic hydrolysate, highlighting the importance of stress response genes and specific genes in the adaptation process. This research provides insights into the molecular mechanisms of short-term adaptation of yeast to lignocellulosic hydrolysate and suggests potential genetic targets for improving fermentation robustness.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Biotechnology & Applied Microbiology
Lisbeth Olsson, Peter Rugbjerg, Luca Torello Pianale, Cecilia Trivellin
Summary: This review discusses the challenges of robustness in microbial cell factories for sustainable production, especially when subjected to industrial conditions. It describes industrial perturbations, state-of-the-art technologies to counter process variability, and the potential of single-cell studies for improving system robustness. The importance of achieving consistent and comparable quantification of robustness for selecting strains in industrial bioprocesses is highlighted.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Sachin Vyas, Maurizio Bettiga, Ulrika Rova, Paul Christakopoulos, Leonidas Matsakas, Alok Patel
Summary: This study preliminarily analyzed the squalene synthase gene in thraustochytrids using bioinformatic tools, and characterized and predicted its features through sequence alignment and the construction of secondary and 3-D models.
Article
Biochemical Research Methods
Veronica Saez-Jimenez, Simone Scrima, Matteo Lambrughi, Elena Papaleo, Valeria Mapelli, Martin K. M. Engqvist, Lisbeth Olsson
Summary: Pathway engineering is commonly used to improve the production of metabolites, but low catalytic activity can create bottlenecks in specific reaction steps. This study successfully obtained mutant variants of (R)-2-hydroxyglutarate dehydrogenase with a 100-fold higher catalytic efficiency, paving the way for the bio-based production of adipic acid. Rational analysis revealed that mutation A206V played a key role in the improved activity.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Scott Mazurkewich, Karoline C. Scholzen, Rikke H. Brusch, Jens-Christian N. Poulsen, Yusuf Theibich, Silvia Huttner, Lisbeth Olsson, Johan Larsbrink, Leila Lo Leggio
Summary: In plant cell walls, covalent bonds between polysaccharides and lignin increase recalcitrance to degradation. Ester bonds between glucuronic acid moieties on glucuronoxylan and lignin can be cleaved by glucuronoyl esterases (GEs) from carbohydrate esterase family 15 (CE15). The fungus Lentithecium fluviatile encodes three CE15 enzymes, and one of them, LfCE15C, has been investigated in detail. Although no activity was detected on any tested substrate, the structure of LfCE15C suggests its potential to bind and act on more extensively decorated xylan chains.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)