Article
Biochemistry & Molecular Biology
In Jung Kim, Uwe T. Bornscheuer, Ki Hyun Nam
Summary: In this study, the biochemical and structural analysis of a Bgl enzyme (TsaBgl) from the hemicellulose-degrading bacterium Thermoanaerobacterium saccharolyticum was reported. TsaBgl exhibited maximum hydrolase activity at pH 6.0 and 55°C and showed a unique structure compared to other known Bgls. The study improved our understanding of the inhibition and glucose tolerance mechanisms of Bgl enzymes, which is crucial for their industrial application.
Article
Multidisciplinary Sciences
Christopher D. Herring, Maulana Permana Ajie, Lee R. Lynd
Summary: Cocultures of engineered thermophilic bacteria can ferment lignocellulose without costly pretreatment or added enzymes, an ability that can be exploited for low cost biofuel production from renewable feedstocks. The hemicellulose-fermenting species Thermoanaerobacterium thermosaccharolyticum was engineered for high ethanol yield, but we found that the strains switched from growth-coupled production of ethanol to growth uncoupled production of acetate and 1,2-propanediol upon growth cessation, producing up to 6.7 g/L 1,2-propanediol from 60 g/L cellobiose. Deletion of genes encoding methylglyoxal synthase and glycerol dehydrogenase eliminated propanediol production. The inhibition of alcohol and aldehyde dehydrogenase activities by ATP suggests a mechanism of metabolic redirection.
SCIENTIFIC REPORTS
(2023)
Article
Agricultural Engineering
Jin-Rong Huang, Xiong Chen, Bin-Bin Hu, Jing-Rong Cheng, Ming-Jun Zhu
Summary: This study successfully enhanced hydrogen production from sugarcane bagasse by bioaugmentation with MJ2 and addition of biochar. The mechanism of enhancement was further investigated.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Xing Yan, Jie Bu, Xiong Chen, Ming-Jun Zhu
Summary: Microbial extracellular respiration, including Fe(III) and electrode respiration, was observed in Thermoanaerobacterium thermosaccharolyticum MJ2. Comparative genomic analysis revealed the intra/extracellular electron transfer mechanism of MJ2, with hydrogenase and cytochrome c playing crucial roles. Moreover, the addition of exogenous Cyt-c significantly increased the bioelectricity generation by MJ2.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Physical
Yu Litti, M. A. Potekhina, E. A. Zhuravleva, A. V. Vishnyakova, D. S. Gruzdev, A. A. Kovalev, D. A. Kovalev, I. Katraeva, S. N. Parshina
Summary: In this study, a hydrogen-producing bacterium SP-H2 was isolated from municipal sewage sludge and identified as Thermoanaerobacterium thermosaccharolyticum based on its 16S rRNA gene sequence. The bacterium showed the maximum growth rate at 55-60 degrees C and pH 7.5. It exhibited high hydrogen production yields and rates when using various sugars and real wastewaters. The findings suggest that T. thermosaccharolyticum SP-H2 is a promising strain for efficient hydrogen production from carbohydrate-rich unsterile wastewater under thermophilic conditions.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Energy & Fuels
Yi-Rui Yin, Peng Sang, Min Xiao, Wen-Dong Xian, Zhou-Yan Dong, Lan Liu, Li-Quan Yang, Wen-Jun Li
Summary: ThBGL1A, a beta-glucosidase from Thermobifida halotolerans YIM 90462(T), exhibited cold-adapted, salt- and glucose-tolerant characteristics, showing potential applications in commercial cellulose utilization and the bioenergy industry.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Agricultural Engineering
Jing Mai, Bin-Bin Hu, Ming-Jun Zhu
Summary: This study found that in a co-culture system, the bacterium MJ1 utilizes sugars produced from substrate degradation to generate more hydrogen. In addition, MJ1 also alleviates the inhibition of sugar on DSM 1313, resulting in higher hydrogen production efficiency.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yi He, Chenxi Wang, Ronghu Jiao, Qinxue Ni, Yan Wang, Qianxin Gao, Youzuo Zhang, Guangzhi Xu
Summary: In this study, a glucose-tolerant beta-glucosidase gene (Bgl1973) was cloned from Leifsonia sp. ZF2019 and expressed heterologously in E. coli. The enzyme showed excellent salt tolerance and glucose tolerance with high specific activity. Molecular docking analysis revealed the characteristics of its glucose binding location and active channel geometry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Anjali Purohit, Lata Pawar, Sudesh Kumar Yadav
Summary: A gene, glu1 (WP_243232135.1), coding for beta-glucosidase from Microbacterium sp. CIAB417 was studied for its cold adaptive nature and tolerance to high levels of glucose and ethanol. The phylogenetic analysis revealed its close association with a similar gene from Microbacterium indicum. The purified recombinant GLU1 enzyme displayed optimal activity and stability at pH 5 and temperature 30 degrees C, and showed high efficiency in hydrolyzing celloologosaccharides and other substrates. This cold adaptive GLU1 with high glucose and ethanol tolerance could have significant applications in various industries.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Microbiology
L. Popova, H. Bahl, M. A. Egorova, M. R. Leont'eva, A. Netrusov, E. A. Tsavkelova
Summary: Bacteria assigned to the genus Thermoanaerobacterium were isolated from laboratory methanogenic thermophilic cellulolytic microbial communities producing biogas from paper substrates, showing cellulolytic activity on microcrystalline cellulose and filter paper. Phylogenetic analysis revealed that isolates I2 and I3 clustered together, while isolate I1 formed a separate branch. Isolate I2 was unique in producing an insoluble yellow affinity substance similar to one produced by certain anaerobic cellulolytic bacteria.
Article
Plant Sciences
Arman Syah Goli, Vilasinee Hirunpanich Sato, Hitoshi Sato, Savita Chewchinda, Jiraporn Leanpolchareanchai, Jannarin Nontakham, Jantana Yahuafai, Thavaree Thilavech, Pongsatorn Meesawatsom, Metawee Maitree
Summary: This study demonstrates the antidiabetic and antioxidant effects of aqueous extract of Lysiphyllum strychnifolium (LS) leaves in vivo and in vitro.
PHARMACEUTICAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Sreyneang Nhim, Rattiya Waeonukul, Ayaka Uke, Sirilak Baramee, Khanok Ratanakhanokchai, Chakrit Tachaapaikoon, Patthra Pason, Ya-Jun Liu, Akihiko Kosugi
Summary: This research has identified a bacterial strain, A9, that secretes beta-glucosidase and can effectively degrade cellulose in combination with Clostridium thermocellum. The beta-glucosidase enzyme, rTcBG1, shows high thermostability and glucose tolerance, indicating its potential use in a new biological cellulose saccharification process that does not require supplementation with beta-glucosidases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shuaifeng Liu, Meng Zhang, Dengwang Hong, Zemin Fang, Yazhong Xiao, Wei Fang, Xuecheng Zhang
Summary: To obtain β-glucosidases with high cellobiose hydrolysis activity and glucose stimulating properties, a semi-rational strategy was employed to engineer β-glucosidase Bgl2A. A total of 18 mutants were constructed, and A22S, V224D, and A22S/V224D showed improved specific activities and kcat values compared to Bgl2A. A22S exhibited the highest amount of glucose production from cellobiose, while A22S/V224D lost the glucose stimulating property. A22S can be used as a component of enzyme cocktail to match mesophilic cellulases for efficient cellulose hydrolysis.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Energy & Fuels
Xin Liu, Xianpu Zhu, Dominic Yellezuome, Ronghou Liu, Xuwei Liu, Chen Sun, Mohamed Hemida Abd-Alla, Abdel-Hamied M. Rasmey
Summary: In this study, different dosages of Thermoanaerobacterium thermosaccharolyticum were added in the hydrogen production stage of a two-stage anaerobic digestion system. It was found that the addition of 1.12 g Thermoanaerobacterium thermosaccharolyticum had the best effect, significantly increasing the cumulative hydrogen and methane yields. The microbial community structure changed in both the hydrogen production and methane production stages, leading to improved efficiency of substrate decomposition and methane production.
Article
Microbiology
Zedong Jiang, Liufei Long, Meifang Liang, Hebin Li, Yanhong Chen, Mingjing Zheng, Hui Ni, Qingbiao Li, Yanbing Zhu
Summary: In this study, a new beta-glucosidase MaGlu1A was cloned from a marine bacterium, showing optimal activity at 40 degrees C and pH 4.5, substrate preference for aryl-beta-glycosidic bonds with glucose, fucose, and galactose moieties, and strong stimulation by supplemental glucose. The enzymatic characterization of MaGlu1A provides valuable information for its practical applications.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Green & Sustainable Science & Technology
Xinyi Zhang, Kexin Zhu, Hao Shi, Xun Wang, Yu Zhang, Fei Wang, Xun Li
Summary: This study successfully achieved large-scale production of cis-abienol using Escherichia coli cells. By screening and coordinating alcohol kinases and isopentenyl phosphate kinases (IPKs), the cis-abienol yield was significantly improved. Furthermore, by constructing a double-phosphatase-deficient strain (BD203) and optimizing the fed-batch fermentation process, the cis-abienol titer was further increased.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Materials Science, Multidisciplinary
Daihui Zhang, Zenghui Cheng, Shishuai Gao, Hao Shi
Summary: High-performance and multifunctional strain sensors were prepared by a simple fabrication process using vanillin based vitrimers synthesized in one-pot reactions. The study investigated the reaction kinetics, physicochemical properties, and dynamic natures of the vitrimers, and demonstrated the tunability of their mechanical properties. By enhancing the interfacial interaction and utilizing the dynamic feature of Schiff base, strain sensors with sandwich-structures were facilely fabricated, showing a wide linear response range and a high gauge factor.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Biotechnology & Applied Microbiology
Hao Shi, Feng Gao, Xing Yan, Qingfei Li, Xinling Nie
Summary: This study characterized a glycoside hydrolase 51 family alpha-L-arabinofuranosidase (TtAbf51) from thermophile Thermoanaerobacterium thermosaccharolyticum DSM 571. The enzyme showed optimal activity at 55℃ and pH 5.5, and exhibited stability at pH 5.0-8.0 and up to 60℃. It was inhibited by Cu2+, Zn2+, and SDS. The enzyme had residual activity in the presence of arabinose and showed good catalytic effect for both synthesized and natural substrates.
Article
Environmental Sciences
Zhongbiao Tan, Gang Chen, Yipin Zhao, Hao Shi, Shiyan Wang, Muhammad Bilal, Dong Li, Xiangqian Li
Summary: Converting renewable biomass into carbon-neutral biofuels is an effective strategy for achieving zero carbon emissions and protecting the environment. Microorganisms with high methanol-tolerant lipase activity were screened and identified from soil, providing robust biocatalysts for efficient biodiesel production and other industrial applications.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Jia Zhou, Jiahui Wang, Mengdie Yao, Junyi He, Yuxiang Yang, Xiangqian Li, Zhongbiao Tan, Hao Shi, Xiaoyan Zhu, Baoxia Tian
Summary: A novel pathway for isopropanol synthesis was discovered, which is acetate-independent. By knocking out certain genes, the production of isopropanol can be further increased. This study provides a new perspective for biosynthesis of isopropanol.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Xinling Nie, Yan Xing, Qingfei Li, Feng Gao, Shiyan Wang, Pei Liu, Xiangqian Li, Zhongbiao Tan, Pixiang Wang, Hao Shi
Summary: In this study, the rapid method of atmospheric and room temperature plasma (ARTP) was used for mutagenesis of Saccharomyces boulardii, and the medium for cultivation was further optimized. The mutant yeast showed high biomass and selenium accumulation, indicating great potential applications in the food, medical, and pharmaceutical industries.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xing Yan, Xinling Nie, Qingfei Li, Feng Gao, Pei Liu, Zhongbiao Tan, Hao Shi
Summary: This study focused on the bio-characterization and assessment of a GH38 alpha-mannosidase from the hyper-thermophile Pseudothermotoga thermarum DSM 5069. The enzyme showed stability and activity at specific pH and temperature ranges, and required cobalt ions for catalysis.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yang Liu, Yangbao Wu, Linguo Zhao, Jianjun Pei
Summary: This article reports a soluble prenyltransferase gene (NgFPT) that can achieve structural modification of flavonoids and enhance their bioactivity and bioavailability. By constructing recombinant strains and optimizing reaction conditions, efficient production of 3'-C prenylated flavonoids was achieved.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Editorial Material
Agricultural Engineering
Zhenjie Sun, Shanjian Liu, Yue Xu, Jingqi Lu, Hao Shi, Songlin Li, Chuping Luo, Qing Dong
Summary: Urea was added to fungal pretreated corn stover and then pyrolyzed to produce nitrogen-containing bio-oil and nitrogen-doped biochar. The enrichment of nitrogen-containing compounds in the bio-oil was effectively achieved through urea soaking, with a maximum content of 66.32% under 4 wt% urea concentration. Amines were the dominant component among the nitrogen-containing compounds, with a maximum content of 41.17%. Higher urea concentration facilitated more nitrogen fixation in the biochar, resulting in a nitrogen content of 12.86 wt% under 8 wt% urea concentration. Nitrogen on the biochar surface existed in the form of pyrrolic-N, pyridinic-N, and graphite-N. In conclusion, urea soaking on fungus pretreated biomass for pyrolysis is a promising method to obtain high value-added nitrogen-containing chemicals and nitrogen-doped biochar with high nitrogen content.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Xinling Nie, Xurui Yang, Junyi He, Pei Liu, Hao Shi, Tao Wang, Daihui Zhang
Summary: In recent years, microbial conversion of inorganic selenium into a safe and efficient form of organic selenium has been a focus of research. Selenium nanoparticles derived from nanotechnology offer higher safety, absorption, and biological activity compared to other selenium forms. This paper reviews the conversion of inorganic selenium to less toxic organic selenium and the biosynthesis of selenium nanoparticles by microbes. The synthesis method, potential mechanism, and characterization of selenium in different forms are also discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Agriculture, Multidisciplinary
Feiyin Ge, Gang Chen, Minjing Qian, Cheng Xu, Jiao Liu, Jiaqi Cao, Xinchao Li, Die Hu, Yangsen Xu, Ya Xin, Dianlong Wang, Jia Zhou, Hao Shi, Zhongbiao Tan
Summary: With the development of artificial intelligence, methods for enzyme engineering have been expanded. Various network models and algorithms have been used to optimize lipase production and properties. However, there is still a research gap in utilizing AI to engineer lipase.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Chemistry, Physical
Zhongbiao Tan, Gang Chen, Silu Chen, Jiaoyang Zhang, Junjie Liu, Xincheng Ma, Hualan Liao, Zhixuan Hu, Feiyin Ge, Fang Ju, Hao Shi, Muhammad Bilal
Summary: Increasing environmental concerns and demand for industrial fuels require the development of alternate and sustainable energy sources. Biodiesel, a renewable and environmentally friendly fuel, is produced by trans-esterifying various feedstocks. Lipases, robust enzymes, can catalyze a range of reactions involved in biodiesel synthesis. Protein engineering techniques can improve the catalytic characteristics of lipases, such as stability, activity, and substrate specificity, enhancing their industrial applicability. This review provides an overview of recent advancements in lipase engineering for biodiesel synthesis.
MOLECULAR CATALYSIS
(2023)
Review
Engineering, Environmental
Gang Chen, Feiyin Ge, Yao Wang, Pei Liu, Shuai He, Hao Shi, Zhongbiao Tan
Summary: Selenium is an essential nutrient for humans, but excessive consumption is toxic and harmful. With severe Se pollution in water environments, it is necessary to find appropriate methods to remove Se from industrial waste. Adsorption using magnetic nano-particles is an effective and cost-saving method for removing dissolved-Se, and these particles can be easily recycled due to their superparamagnetic properties.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Agricultural Engineering
Chunbao Chen, Dianlong Wang, Ya Xin, Hao Shi, Qiaoxia Yuan
Summary: This study investigated the production of hydrogen-rich gas from cattle manure char through steam gasification. The characteristics of char were analyzed and the effects of various factors on gas yields and composition were explored. The results showed that high temperature and fast heating led to higher yields of syngas and hydrogen. Moreover, the presence of certain catalysts improved gas production, while others inhibited it. The optimal parameters for gasification were determined. Compared to biomass, char exhibited better performance in terms of gas and hydrogen production. Thus, steam gasification of char holds promise as a method for producing hydrogen-rich gas.
JOURNAL OF THE ASABE
(2023)
Review
Biotechnology & Applied Microbiology
Honglin Lu, Maoyuan Xue, Xinling Nie, Hongzheng Luo, Zhongbiao Tan, Xiao Yang, Hao Shi, Xun Li, Tao Wang
Summary: This review illustrates the composition and structure of lignocellulose, as well as enzyme sources and new technologies for efficient hydrolysis. Converting abundant natural biomass into fermentable sugars or other intermediates has great practical applications.