期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 18, 期 5, 页码 888-896出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-013-0044-7
关键词
Candida rugosa lipase; hollow silica microsphere; dextran; immobilization; resolution
资金
- National Natural Science Foundation of China [20773153, 50872153]
- National Basic Research Program of China [2009CB226101, 2009CB724706, 2010CB234601, 2011CB201403]
Candida rugosa lipase (CRL) is one of the most widely used lipases. To enhance the catalytic abilities of CRL in both aqueous and non-aqueous phases, hollow silica microspheres (HSMSs) with a pore size of 18.07 nm were used as an immobilization support, and aldehydecontaining dextrans were employed to further cross-link the adsorbed CRL. In the experimental ranges examined, the loading amount of lipase linearly increased to 171 +/- 3.4 mg(protein)/g(support) with the CRL concentration and all the adsorption equilibriums were reached within 30 min. After simple cross-linking, the tolerance to pH 4.0 similar to 8.0 as well as the thermal stability of immobilized CRL at 40 similar to 80A degrees C were both substantially increased, and 82 +/- 2.1% activity remaining after the sixth reuse. The immobilized CRL was successfully applied to the resolution of racemic ibuprofen in non-aqueous phase. The initial reaction rate increased by 1.4- and 3.6-fold compared with the rates of adsorbed and native lipases, respectively. Furthermore, the R-ibuprofen was obtained at ee > 93%, and the enantiomeric ratio reached E > 140 at the conversion of 50 +/- 1.5% within 48 h.
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