期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 18, 期 6, 页码 1062-1066出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-013-0282-8
关键词
Escherichia coli; gamma-aminobutyric acid; glutamate decarboxylase; Neurospora crassa
资金
- Next-Generation BioGreen 21 Program (SSAC), Rural Development Administration, Republic of Korea [PJ00954904]
This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30 degrees C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.
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