Article
Biotechnology & Applied Microbiology
Sahana Vasudevan, Parthasarathy Srinivasan, Prasanna Neelakantan, John Bosco Balaguru Rayappan, Adline Princy Solomon
Summary: In this study, a novel photoluminescence-based assay using cysteamine-capped titanium oxide nanoparticles was developed for the rapid and sensitive diagnosis of microbial infections. The system showed a maximum relative response of 98% for AHLs produced by Pseudomonas aeruginosa, with a linear detection range of 10-160 nM in simulated host urine. The efficacy and specificity of the system in detecting AHLs produced by P. aeruginosa were confirmed in a temporal manner.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Raman Pachaiappan, Tharun Prasanna Rajamuthu, Ananya Sarkar, Pradiksha Natrajan, Nagasathiya Krishnan, Meenakumari Sakthivelu, Palaniyandi Velusamy, Palaniappan Ramasamy, Subash C. B. Gopinath
Summary: Flavonoids and isoflavonoids are effective inhibitors of biofilm formation and virulence factor production in Pseudomonas aeruginosa, with acacetin exhibiting the strongest inhibition capacity. These compounds downregulate the expression of virulence genes, making them potential targets for anti-quorum sensing strategies.
PROCESS BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Rachith Kalgudi, Roya Tamimi, Godfrey Kyazze, Tajalli Keshavarz
Summary: The study revealed that the combined use of farnesol and tyrosol had a better effect on Pseudomonas aeruginosa biofilm growth, especially against the RBHi strain, while the addition of quorum quenchers (QQs) reduced IL-8 secretion by A549 cells.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ankita Chopra, Dattatray Mongad, Surekha Satpute, Pranab Behari Mazumder, Praveen Rahi
Summary: The study aimed to isolate and characterize tea rhizobacteria that produce quorum sensing molecules and exhibit multiple plant growth-promoting activities. Thirty-one isolates were obtained from the tea rhizosphere, and two isolates (RTE1 and RTE4) were selected for their ability to inhibit tea fungal pathogens and produce AHL molecules. Genome-based analysis confirmed RTE1 as Burkholderia cepacia and RTE4 as Pseudomonas aeruginosa. Detailed characterization of the AHLs produced by the isolates revealed different chain lengths. Confocal microscopy showed biofilm formation by RTE1 and RTE4. Several genes involved in quorum sensing, plant growth promotion, and inducing systemic resistance were identified. Based on their functional traits, B. cepacia RTE1 and P. aeruginosa RTE4 are promising candidates for enhancing the health and growth of tea plantations.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
M. de Celis, L. Serrano-Aguirre, I Belda, R. Liebana-Garcia, M. Arroyo, D. Marquina, I de la Mata, A. Santos
Summary: The study indicates that the use of AHLs-degrading enzymes can reduce biofilm formation on WWTP membranes and ameliorate the virulence of Gram-negative bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agricultural Engineering
Shamas Tabraiz, Burhan Shamurad, Evangelos Petropoulos, Marcos Quintela-Baluja, Alex Charlton, Jan Dolfing, Paul J. Sallis
Summary: The novel QQ strain Acinetobacter guillouiae ST01 showed high activity in reducing quorum sensing in a membrane bioreactor by decreasing AHL concentrations in the sludge and biofilm. It also significantly reduced polysaccharide and protein levels, leading to a decrease in fouling rates in the MBR. These results suggest that A. guillouiae ST01 is a promising candidate for biofouling control in wastewater treatment.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Zahid Ur Rehman, Afaque A. Momin, Abdullah Aldehaiman, Tayyaba Irum, Raik Grunberg, Stefan T. Arold
Summary: Quorum quenching (QQ) is the enzymatic degradation of molecules used by bacteria for synchronizing their behavior within communities. QQ has potential applications in water treatment, aquaculture, and healthcare due to its ability to inhibit biofilm formation and suppress the production of virulence factors. LrsL is a metallo-beta-lactamase superfamily QQ enzyme with exceptional catalytic features and high affinity for acyl-homoserine lactones (AHLs).
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Lara Serrano-Aguirre, Rodrigo Velasco-Bucheli, Begona Garcia-Alvarez, Ana Saborido, Miguel Arroyo, Isabel de la Mata
Summary: This study identified a novel N-acylhomoserine lactone acylase from Actinoplanes utahensis, which can hydrolyze and synthesize various natural compounds, showing potential as a quorum quenching agent. This enzyme inhibits quorum sensing processes and can efficiently synthesize AHLs, highlighting its potential for the development of new antimicrobials.
Article
Environmental Sciences
Xiyuan Xu, Shuai Wang, Chengxuan Li, Jingxi Li, Fenglei Gao, Li Zheng
Summary: Microplastics in the oceans are a new type of pollutant with complex biofilms. This study investigated the diversity and biological characteristics of quorum sensing bacteria in microplastic-associated biofilms, and found that these bacteria have strong biofilm-forming capacity and potential to exacerbate adverse environmental effects.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
You Mit Prohim, Roent Dune A. Cayetano, Parthiban Anburajan, Nguyen Tang Thau, Sungmi Kim, Hyun-Suk Oh
Summary: Biomethane recovered through anaerobic digestion (AD) is a renewable and cost-effective alternative energy source that can help address rising energy demands. This study investigates the role of quorum sensing (QS) in AD systems and finds that the addition of signaling molecules (AHLs) enhances methane yield and promotes microbial growth and cell aggregation, ultimately improving biomethane synthesis.
Article
Environmental Sciences
I-Chieh Chien, Chiao-Yun Chu, Chien-Yu Huang
Summary: Membrane bioreactors (MBRs) are widely used in wastewater treatment due to their high-quality effluent production. However, biofilm formation on membrane surfaces remains a major limitation of this technique. Bacterial quorum quenching (QQ) has been proven successful in controlling biofouling in MBRs. This study isolated potential QQ bacteria and investigated their degradation rates against eight different AHLs. The results showed that four isolates exhibited consistent AHL removal capabilities, indicating their potential for mitigating biofilm formation on MBR membranes.
Article
Immunology
Pamela Ruiz, Daniela Sepulveda, Jose Miguel Vidal, Romina Romero, David Contreras, Javier Barros, Carlos Carrasco, Nathaly Ruiz-Tagle, Alex Romero, Homero Urrutia, Cristian Oliver
Summary: The study detected AHLs produced by P. salmonis using a fluorescent biosensor system and GC/MS. The results showed the presence of AHL-type molecule in both P. salmonis strains and suggested a possible alternate pathway for AHL synthesis. This report is the first to describe the type of AHLs produced by P. salmonis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Xu Wang, Cancan Jiang, Danhua Wang, Yang Yang, Lijing Fan, Shengjun Xu, Xuliang Zhuang
Summary: Free nitrous acid (FNA) is widely used for wastewater management by altering sludge characteristic and function. This study investigated the role of collective behaviors in FNA treatment and revealed the impact of FNA on sludge morphology and composition.
Article
Agricultural Engineering
Xiaojing Zhang, Bingbing Ma, Nan Zhang, Han Zhang, Yongpeng Ma, Yali Song, Hongzhong Zhang
Summary: This study investigated the regulatory effect of the external quorum sensing signals, N-dodecanoyl homoserine lactone (C12-HSL), on the CANON process in a membrane bioreactor. C12-HSL enhanced the activity of aerobic ammonia-oxidizing bacteria and improved ammonia monooxygenase activity, while suppressing anaerobic ammonia-oxidizing bacteria and limiting TN removal. Enzyme synthesis was enhanced, enabling the CANON system to resist high C12-HSL concentrations. C12-HSL was found to be profitable for aerobic ammonia oxidation and could be used to regulate nitrite production.
BIORESOURCE TECHNOLOGY
(2023)