期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 15, 期 2, 页码 299-307出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-009-0117-9
关键词
Hericium erinaceus; doehlert design; submerged culture; medium optimization; biomass; exo-polymer
资金
- Technological Research and Development Program of Zhenjiang City [GY2007028]
- Jiangsu Education Department Natural Sciences Foundation of China [07KJD350034]
- Natural Science Foundation of Zhejiang Province [Y306205]
- Research Foundation for Advanced Talents of Jiangsu University [07JDG020]
The Doehlert experimental design was used to optimize the production of mycelial biomass and exo-polymer from Hericium erinaceus CZ-2 in this study. Statistical analysis showed that the linear and quadric terms of 3 variables: corn flour, yeast extract, and corn steep liquor had significant effects. The optimized combination of these 3 variables was confirmed through validation experiments. The optimal conditions for higher production of mycelial biomass (19.92 g/L) were estimated when the media composition concentrations were set as: 30.85 g/L, corn flour; 2.81 g/L, yeast extract; 16.9 mL/L, corn steep liquor; 10 g/L, glucose; 1 g/L, KH(2)PO(4); and 0.5 g/L, MgSO(4)center dot 7H(2)O; while a maximal exo-polymer yield (1.653 g/L) could be achieved when setting concentrations of: 32.71 g/L, corn flour; 2.35 g/L, Yeast extract; 14.42 mL/L, Corn steep liquor; 10 g/L, glucose; 1 g/L, KH(2)PO(4); and 0.5 g/L, MgSO(4)center dot 7H(2)O. The upscale production was also investigated using a 15 L fermentor using the optimized medium.
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