期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 14, 期 1, 页码 46-51出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-008-0091-7
关键词
Thermobifida fusca; mixed acids; cutinase; cultivation strategy
资金
- Program for New Century Excellent Talents in University [NCET-05-0488]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0532]
- National Science Fund for Distinguished Young Scholars of China [20625619]
- 973 Project [2007CB714306]
- Major Technological Research Program of Ministry of Education [V200611]
- 111 Project [111-2-06]
In this study, cutinase production by Thermobifida fusca WSH03-11 was investigated with mixed short-chain organic acids as co-carbon sources to demonstrate the possibility of producing high value-added products from organic wastes. T. fusca WSH03-11 was cultured with different combinations of butyrate, acetate, and lactate with a purpose of increasing cutinase activity. The optimum proportion of butyrate, acetate, and lactate was 4:1:3. In batch cultivation, acetate and lactate were consumed quickly, while the consumption of butyrate was depressed in the presence of acetate with a concentration higher than 0.5 g/L. Based on these results, a two-stage batch and fed-batch cultivation strategy was proposed: a batch culture with acetate and lactate as the co-carbon sources in the first 10 h, and then a fed-batch culture with a constant butyrate feeding rate of 12 mL/h during 11 similar to 20 h. By this two-stage cultivation strategy, cutinase activity, dry cell weight, and consumption rate of butyrate were increased by 70%, 103.4%, and 4.3-fold, respectively, compared to those of the batch cultivation. These results provided a novel and efficient way to produce high value-added products from organic wastes.
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