Article
Plant Sciences
Dingding Cao, Wenbin Zhang, Nan Yang, Ziling Li, Chaoyue Zhang, Dan Wang, Guiping Ye, Jianjun Chen, Xiangying Wei
Summary: This study analyzed the responses of Sesuvium portulacastrum to salt stress at morphological, physiological, proteomic, and metabolomic levels. The results showed that signal transduction and phenolic acid metabolism were crucial for S. portulacastrum's salt tolerance. The study also identified the coordinated regulation of Ca2+, ABA, and JA signaling pathways in promoting salt tolerance in S. portulacastrum.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Medicinal
Jinxin Xie, Lijuan Wang, Xiaoyong Zhang, Yiyang Li, Xin Liao, Caixin Yang, Ri-Yuan Tang
Summary: Aryloxyethyl propiolates and their homologues are promising anti-MRSA agents with multiple targets, as they can destroy the cell wall and cell membrane, inhibit the formation of a biofilm, and bind to the DNA of MRSA.
ACS INFECTIOUS DISEASES
(2022)
Article
Engineering, Environmental
Lei Zhang, Xiujuan Li, Wenlu Zuo, Shuang Li, Gangzheng Sun, Weidong Wang, Yadong Yu, He Huang
Summary: The study revealed that low-molecular-weight organic acids can enhance the biodegradation of PAHs, promote cell growth and activity, and alleviate membrane and oxidative stress.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Medicine, General & Internal
Songyan Gao, Yufan Chao, Na Li, Henghui Li, Hongxia Zhao, Xinru Liu, Wei Chen, Xin Dong
Summary: Renal fibrosis is an important process of chronic kidney disease (CKD) progressing to end-stage renal failure. Oxalate crystal-induced kidney injury is an early stage of nephrolithiasis. This study revealed the mechanism of crystal kidney injury and provided potential targets for the intervention of nephrolithiasis.
FRONTIERS IN MEDICINE
(2022)
Article
Gastroenterology & Hepatology
Xingyu Yao, Shiyu Xiao, Liya Zhou
Summary: This study reveals that bismuth inhibits the growth of H. pylori by downregulating virulence factors, disrupting flagella assembly, and inhibiting antioxidant enzymes. Additionally, bismuth treatment impairs various metabolic pathways in H. pylori, affecting biological processes related to growth and translation. This provides insights into how bismuth eradicates H. pylori in quadruple therapy.
Article
Nanoscience & Nanotechnology
Min-Quan Hui, Yan-Ni Mi, Yu-Fan Ma, Tao Chen, Yong-Xiao Cao
Summary: The 13V-LE formulation is a suitable intravenous lipid emulsion without anaphylactoid reactions. It contains 13 fat- and water-soluble vitamins and is composed of soybean oil, glycerin, egg lecithin, and purified water with stable zeta-potential and high entrapment efficiency.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Multidisciplinary Sciences
Rotimi Fasimoye, Wentao Dong, Raja S. Nirujogi, Eshaan S. Rawat, Miharu Iguchi, Kwamina Nyame, Toan K. Phung, Enrico Bagnoli, Alan R. Prescott, Dario R. Alessi, Monther Abu-Remaileh
Summary: The Golgi is a crucial organelle involved in protein and lipid biosynthesis, as well as sorting and secretion. Dysfunctional Golgi is associated with various diseases. This study presents a Golgi immunoprecipitation technique to isolate intact Golgi mini-stacks for analysis. The analysis pipeline reveals the molecular features of the human Golgi, including proteome, metabolome, and lipidome profiles. This comprehensive molecular map provides a powerful method to study the Golgi in health and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemical Research Methods
Benjamin C. Orsburn
Summary: Mutations in the KRAS gene are common drivers of cancer in humans. This study discovered a promising compound, MRTX1133, that inhibits the activity of KRAS(G12D) mutant proteins in pancreatic cancer cells. Through proteomics and metabolomics analysis, it was found that after 48 hours of treatment, the compound showed different levels of effectiveness in reducing the abundance of KRAS protein in different cell populations.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Clinical Neurology
Dominique F. Leitner, Yik Siu, Aryeh Korman, Ziyan Lin, Evgeny Kanshin, Daniel Friedman, Sasha Devore, Beatrix Ueberheide, Aristotelis Tsirigos, Drew R. Jones, Thomas Wisniewski, Orrin Devinsky
Summary: High-fat and low-carbohydrate diets, such as the modified Atkins diet (MAD), can reduce seizure frequency in treatment-resistant epilepsy patients. These diets mimic fasting and induce high levels of ketone bodies, potentially shifting brain energy production and impacting neuronal and astrocyte metabolism.
Article
Medicine, General & Internal
Ning Wang, Yaozhong Zhang, Yu Hu, Qiyao Yang, Zhenbo Su
Summary: Although anaphylactoid reactions caused by cis-atracurium are rare, the bronchospasm and anaphylactic shock it induces greatly increase the risk of anesthesia, which should be taken seriously by clinicians. Increased vigilance in diagnosis and treatment is essential to prevent aggravation and further complications.
Article
Engineering, Environmental
Ye Zhu, Yukang Zhang, Yanbo Li, Caixia Guo, Zhuying Fan, Yang Li, Man Yang, Xianqing Zhou, Zhiwei Sun, Ji Wang
Summary: This study investigated the effects of silica nanoparticles (SiNPs) on the metabolic spectrum in hepatocytes, revealing the metabolic mechanisms underlying SiNP-induced hepatotoxicity. Integrative omics analyses proved to be a powerful approach for toxicity evaluations and risk assessments of nanoparticles.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Multidisciplinary Sciences
Lei Li, Lin Huang, Chenyang Huang, Jia Xu, Yukai Huang, Haihua Luo, Xinya Lu, Shuyue He, Gang Yuan, Li Chen, Xue Han, Xusong Cao, Aolin Jiang, Cuiting Liu, Junmin Shi, Hong Yang, Yong Jiang
Summary: This study focused on the molecular dynamics profiles of exosomes in serum during the development of sepsis. High-performance liquid chromatography-tandem mass spectrometry and RNA-seq were used to detect the dynamic profiles of exosome proteins and RNAs. Integrative multiomics analyses revealed the association of exosome components with various biological processes and molecular pathways. Importantly, exosomes derived from septic serum showed potential therapeutic effects in septic mice.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Environmental Sciences
Saurabh Gangola, Pankaj Bhatt, Alagarasan Jagadeesh Kumar, Geeta Bhandari, Samiksha Joshi, Arjita Punetha, Kalpana Bhatt, Eldon R. Rene
Summary: Pesticides have negative effects on the environment and human health, but biological approaches have proven to be effective in removing pesticide contamination. There is a lack of understanding on the factors affecting microbial degradation ability, and the use of advanced tools and omics technology can help fill this knowledge gap.
Review
Plant Sciences
Xiangyu Xu, Cassio Flavio Fonseca de Lima, Lam Dai Vu, Ive De Smet
Summary: Changes in weather patterns, drought risks, and rising global temperature negatively impact crop growth and productivity. Plants are exposed to multiple stresses simultaneously, but most studies focus on individual stress conditions. This mini-review focuses on the combination of drought and heat, summarizing current research results, identifying knowledge gaps, and suggesting potential future focus areas.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Chi Zhang, Linqi Ouyang, Xili Zhang, Wen Wen, Yuqin Xu, Shan Li, Yingyu Li, Fuyuan He, Wenlong Liu, Hongyu Liu
Summary: The study found that both SHLI and SMI can induce different degrees of anaphylactoid reactions, and the intensity of the reaction is positively correlated with the dose. Metabolomics research found that SHLI and SMI may promote the simultaneous release of hormones and inflammatory factors by disturbing relevant metabolic pathways, while SMI may also inhibit the release of inflammatory factors in the arachidonic acid metabolic pathway, indicating both pro-inflammatory and anti-inflammatory effects.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Medicine, General & Internal
Yubin Xu, Na Zhang, Shanshan Xu, Hongyan Xu, Saizhen Chen, Zhelin Xia
Review
Biotechnology & Applied Microbiology
Xiaojun Wang, Na Zhang, Yubin Xu
BIOMED RESEARCH INTERNATIONAL
(2020)
Article
Biochemistry & Molecular Biology
Yubin Xu, Saizhen Chen, Jinguang Chen
Summary: In this study, various datasets were analyzed to identify transcriptomic signatures and key regulators of atopic dermatitis (AD). The results revealed genes related to immune response and dermal integrity, overrepresented signaling pathways in lesional AD, and the roles of regulators in epidermal integrity and immune response. The study also suggested a potential role of B cell-mediated immune response in the pathogenesis of AD, in addition to the well-known T cell-mediated immune response. Furthermore, small molecular drugs were identified as potential therapeutic targets for AD based on the analysis.
MOLECULAR GENETICS AND GENOMICS
(2021)
Article
Computer Science, Software Engineering
Yu-Bin Xu
Summary: By utilizing network pharmacology, Huanglian Jiedu Decoction was shown to act against atopic dermatitis through a multi-component, multi-target, and multi-pathway mode of action, significantly reducing the levels of IL-4, IL-6, and IL-10 in T lymphocytes of mice with atopic dermatitis.
SCIENTIFIC PROGRAMMING
(2021)
Article
Chemistry, Analytical
Zhen Xiong Zhao, Sai Zhen Chen, Zhe Lin Xia, Yu Bin Xu, Ling Ling Zhang, Shan Ming Tian, Qing Fan
Summary: In this study, a gas chromatography-mass spectrometry method was successfully established to detect N-nitrosamines (NAs) in rat feces. The method revealed that the levels of NAS were higher in the feces of rats with colorectal cancer compared to normal rats. Additionally, a high nitrite diet was found to contribute to an abnormal increase in NAs in feces. This work provides an analytical method for further in vivo study of NAs and completes a pilot study on the relationship between NAs and colorectal cancer.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Pharmacology & Pharmacy
Jing Chen, Saizhen Chen, Jinguang Chen, Bixin Shen, Zhengli Jiang, Yubin Xu
Summary: Atopic dermatitis (AD) is a common chronic relapsing skin inflammation that severely impacts patients' quality of life. Huanglian Jiedu decoction (HLJDD) has been shown to have multiple pharmacological effects in the treatment of AD, particularly in inhibiting itching and enhancing immunity. Through a combination of chemistry, biochemistry, and metabolomics strategies, this study identified the active substance basis of HLJDD against AD, with a focus on components such as 40AEF and CPF.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Yubin Xu, Saizhen Chen, Lingling Zhang, Guirong Chen, Jinguang Chen
Summary: The study indicates that Huanglian Jiedu decoction can treat atopic dermatitis by regulating the antigen presentation function of dendritic cells, weakening T-lymphocyte activation, and subsequently exerting anti-inflammatory and anti-pruritus effects.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jia Qian Pang, Ling Ling Zhang, Yu Bin Xu, Qing Fan, Zhen Xiong Zhao
Summary: This study aims to establish a rapid and sensitive LC-MS/MS method for determining the concentration of potential genotoxic impurities (GTIs) in L-malic acid. The method is compliant with ICH guidelines and can be used for routine analysis and stability studies in pharmaceutical quality control.
CURRENT PHARMACEUTICAL ANALYSIS
(2023)
Article
Pharmacology & Pharmacy
Guirong Chen, Xiaobo Wang, Chang Liu, Mingbo Zhang, Xueying Han, Yubin Xu
Summary: This study shows that the active components of Huanglian Jiedu Decoction (HJD) can treat sepsis by inactivating the LPS-TLR4/MD-2-NF-kappa B pathway. It is also found that the active component formulation (ACF) has similar efficacy and mechanism, reducing inflammatory cell infiltration and organ damage. Furthermore, ACF regulates certain metabolites, potentially alleviating physical damage caused by HJD.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Cheng-Guo Ju, Lin Zhu, Wei Wang, Hui Gao, Yu-Bin Xu, Tian-Zhu Jia
Summary: The processing of Cornus officinalis may improve renal injury in DN rats by blocking the activation of the Wnt/beta-catenin signaling pathway and regulating the structural composition of intestinal microbiota.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Zhen Xiong Zhao, Xiao Hui Tang, Sheng Lu Jiang, Jia Qian Pang, Yu Bin Xu, Dan Dan Yuan, Ling Ling Zhang, Hui Min Liu, Qing Fan
Summary: This study established a high-purine diet-induced HN rat model and found that the pharmacokinetics of febuxostat showed accumulation in HN rats. Moreover, Astragaloside IV improved the pharmacokinetics of febuxostat in HN rats by regulating gut microbiota. These findings suggest that febuxostat might accumulate in HN patients, and Astragaloside IV could reverse the accumulation through gut microbiota regulation.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Plant Sciences
Zhixiang Liu, Xiaoqing Ma, Nan Zhang, Linlin Yuan, Hongrui Yin, Lingling Zhang, Tong An, Yubin Xu
Summary: In this study, phenylpropanoid amides were isolated from Solanum rostratum and their chemical structures were identified. These substances showed varying levels of inhibitory effects on seed germination and root elongation of Arabidopsis thaliana, especially compounds 2 and 4. Compounds 2 and 4 also displayed potent inhibitory effects on the antioxidant-related enzyme POD.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Chang Liu, Sheng Shu, Zhelin Xia, Guirong Chen, Yubin Xu
Summary: This study explored the real material and mechanisms of Huanglian jiedu decoction (HJD) for atopic dermatitis (AD) using network pharmacology and in vivo experiments. The results showed that HJD contains 60 bioactive compounds interacting with 169 related hub targets and participates in the pathogenesis of AD through the PI3K-Akt signaling pathway, MAPK signaling pathway, Ras signaling pathway, and Fc epsilon RI signaling pathway. In vivo experiments demonstrated that HJD reduces epidermal thickness, inflammatory cell infiltration, and downregulates key indicators in the PI3K/AKT and MAPK pathways.
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2023)
Article
Immunology
Guirong Chen, Chang Liu, Mingbo Zhang, Xiaobo Wang, Yubin Xu
Summary: This study evaluated the potential therapeutic effects of Niloticin and its ability to bind to MD-2, a protein involved in inflammatory responses mediated by TLR4. The results showed that Niloticin could significantly decrease the levels of inflammatory cytokines induced by LPS and inhibit the activation of the LPS-TLR4/MD-2-NF-kappa B signaling pathway.
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
(2022)
Article
Immunology
Guirong Chen, Yunong Liu, Yubin Xu, Mingbo Zhang, Song Guo, Gang Zhang
Summary: Isoimperatorin (QHS), a phytoconstituent from Angelica dahurica roots, exhibits potent anti-inflammatory activity by reducing inflammatory cytokines and mediators release and expression, inhibiting the LPS-TLR4/MD-2-NF-kappa B signaling pathway. It can serve as a potential TLR4 antagonist by blocking MD-2 binding, offering a therapeutic approach for LPS-induced inflammatory responses.
EUROPEAN JOURNAL OF INFLAMMATION
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)