Article
Biotechnology & Applied Microbiology
Sven Goebel, Fabian Kortum, Karim Jaen Chavez, Ingo Jordan, Volker Sandig, Udo Reichl, Jennifer Altomonte, Yvonne Genzel
Summary: Oncolytic viruses (OVs) expressing specific fusion proteins have promising potential for cancer treatment. This study evaluated four suspension cell lines for the production of a novel fusogenic virus and optimized the process. BHK-21 and HEK293SF cells were identified as promising candidates for rVSV-NDV production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Manman Sun, Alex Xiong Gao, Xiuxia Liu, Yankun Yang, Rodrigo Ledesma-Amaro, Zhonghu Bai
Summary: The demand for faster and more efficient protein production has increased in the post-genomic era. This review presents a holistic high-throughput process development strategy and discusses the challenges, limitations, and future research directions in recombinant protein production.
MICROBIAL CELL FACTORIES
(2023)
Article
Multidisciplinary Sciences
Hiroki Tanemura, Kenji Masuda, Takeshi Okumura, Eri Takagi, Daisuke Kajihara, Hirofumi Kakihara, Koichi Nonaka, Ryo Ushioda
Summary: In this study, a novel high-expression promoter was cloned based on transcriptome analysis to significantly enhance mAb production in CHO cells. The promoter showed improved productivity for various IgG subclasses and may be a new method for maintaining protein homeostasis and achieving stable expression of introduced mAb genes.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Gianmarco Barberi, Antonio Benedetti, Paloma Diaz-Fernandez, Daniel C. Sevin, Johanna Vappiani, Gary Finka, Fabrizio Bezzo, Massimiliano Barolo, Pierantonio Facco
Summary: This study aims to use machine learning to integrate process and biological information for early prediction of cell line performance and explore the relationship with metabolic mechanisms, as well as identify biomarkers to accelerate the selection of high performing cell lines.
METABOLIC ENGINEERING
(2022)
Article
Oncology
Diep-Khanh Vo, Alexander Engler, Darko Stoimenovski, Roland Hartig, Thilo Kaehne, Thomas Kalinski, Michael Naumann, Johannes Haybaeck, Norbert Nass
Summary: Understanding the interactions of eukaryotic initiation factors is crucial for determining the aggressiveness of cancer cells, and analyzing these interactions further aids in understanding key steps in protein synthesis.
Article
Environmental Sciences
Gizem Ozkemahli, Pinar Erkekoglu, Ayse Ercan, Naciye Dilara Zeybek, Nilgun Yersal, Belma Kocer-Gumusel
Summary: This study investigates the toxic effects of BPA and/or MEHP on the HepG2 cell line. The results suggest that combined exposure to these chemicals may cause serious toxicological outcomes.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Cell Biology
Shaogui Wang, Xiaojuan Chao, Xiaoxiao Jiang, Tiantian Wang, Yssa Rodriguez, Ling Yang, Pal Pacher, Hong-Min Ni, Wen-Xing Ding
Summary: VMP1 plays a crucial role in the pathogenesis of pancreatitis by regulating autophagic degradation and ER stress, and its deficiency promotes inflammation and fibrosis. The study revealed the importance of VMP1 in modulating pancreatitis through the NFE2L2/Nrf2 pathway.
Article
Biochemistry & Molecular Biology
Song Zhao, Haiping Feng, Dongfang Jiang, Keyan Yang, Si-Tong Wang, Yu-Xin Zhang, Yun Wang, Hongmei Liu, Caixia Guo, Tie-Shan Tang
Summary: This study reveals that excessive ER Ca2+ can directly activate the IRE1a-XBP1 pathway, and inhibition of over-activated IRE1a-XBP1 signaling in TMCO1-deficient cells can lead to significant cell death.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Mini Jeong, Jong-Il Shin, Jaewook Cho, Yong-Joon Jeon, Jin-Hyun Kim, Jeehee Youn, Kyungho Lee
Summary: In this study, the pro-apoptotic effects of docosahexaenoic acid (DHA) in human fibroblast-like synovial cells from rheumatoid arthritis (RA) patients were investigated. The results showed that DHA treatment induced caspase-8-dependent apoptosis in a dose-dependent manner and reduced the TNF-alpha-mediated induction of MMP-9 and IL-1 beta. DHA also induced ER stress and upregulated the expression of death receptor 5 (DR5) through the activation of CHOP. These findings suggest that DHA can induce apoptosis in RA-FLSs through the upregulation of DR5 via the activation of CHOP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Elisa Mariano Pioltine, Camila Bortoliero Costa, Fernanda Fagali Franchi, Priscila Helena dos Santos, Marcelo Fabio Gouveia Nogueira
Summary: This study evaluated the effects of adding TUDCA in the in vitro culture of bovine embryos and found that TUDCA can reduce ER stress and enhance the cryotolerance of bovine embryos.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Berrin Tugrul, Erdal Balcan, Zubeyde Oztel, Fatih Collu, Beyhan Gurcu
Summary: In this study, the effects of doxorubicin and tunicamycin alone or in combination on the subcellular regulation of p53 through MDM, Cul9, and PrP were investigated. The results showed that doxorubicin increased p53, MDM2, and CUL9 levels in a dose-dependent manner, while tunicamycin had varying effects on p53 and MDM2 expression. Combination treatment resulted in increased p53 expression and decreased MDM2 and CUL9 expressions, making the cells more susceptible to apoptosis. PrP may play a role in determining cell fate under ER stress conditions. Further studies are needed to explore the molecular networks involved.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Khaled Trabelsi, Meriem Ben Zakour, Ingo Jordan, Volker Sandig, Samia Rourou, Hela Kallel
Summary: This study demonstrates that efficient production of rabies animal vaccine can be achieved in the AGE1.CR.pIX suspension cell line using a scalable process in chemically defined medium.
Article
Biochemistry & Molecular Biology
Elena G. Varlamova, Mikhail Goltyaev, Valentina N. Mal'tseva, Egor A. Turovsky, Ruslan M. Sarimov, Alexander Simakin, Sergey Gudkov
Summary: Selenium nanoparticles (SeNP) have gained popularity for their high biocompatibility, stability, and selectivity, with numerous studies proving their anticarcinogenic effects. This study investigated the molecular mechanisms regulating SeNP-induced cytotoxic effects on four human cancer cell lines, highlighting varied concentration-dependent outcomes and the induction of apoptosis through different signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Muhammad Rosyid Ridlo, Eui Hyun Kim, Eun Pyo Kim, Geon A. Kim
Summary: In this study, the application of miR-143 (mimics and inhibitors) to porcine parthenogenetically activated embryos showed that the miR-143 inhibitor can improve embryo development to the blastocyst stage, and is associated with increased activity of autophagy and ER-phagy as well as higher expression levels of genes related to embryo quality.
Article
Biotechnology & Applied Microbiology
Sven Goebel, Karim E. E. Jaen, Marie Dorn, Victoria Neumeyer, Ingo Jordan, Volker Sandig, Udo Reichl, Jennifer Altomonte, Yvonne Genzel
Summary: This study presents a proof-of-concept for the production of a recombinant vesicular stomatitis virus-based fusogenic oncolytic virus (rVSV-NDV) at high cell densities (HCD). Optimization studies were carried out in small-scale cultivations using shake flasks, and a perfusion process was established using an acoustic settler for cell retention. The study found that the acoustic settler allowed for continuous harvesting of rVSV-NDV with high cell retention efficiencies and infectious virus titers, resulting in increased volumetric virus productivity compared to batch processes.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)