期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 109, 期 9, 页码 2357-2368出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.24488
关键词
inkjet printing; cartilage tissue engineering; chondrocyte; hydrogel; extracellular matrix; photoplymerization
资金
- NIH [AG007996]
- CIRM [TR1-01216]
- STSI [UL1 RR025774]
- NSF [1011796]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1011796] Funding Source: National Science Foundation
Bioprinting as a promising but unexplored approach for cartilage tissue engineering has the advantages of high throughput, digital control, and highly accurate placement of cells and biomaterial scaffold to the targeted 3D locations with simultaneous polymerization. This study tested feasibility of using bioprinting for cartilage engineering and examined the influence of cell density, growth, and differentiation factors. Human articular chondrocytes were printed at various densities, stimulated transiently with growth factors and subsequently with chondrogenic factors. Samples were cultured for up to 4 weeks to evaluate cell proliferation and viability, mechanical properties, mass swelling ratio, water content, gene expression, ECM production, DNA content, and histology. Bioprinted samples treated with FGF-2/TGF-beta 1 had the best chondrogenic properties among all groups apparently due to synergistic stimulation of cell proliferation and chondrogenic phenotype. ECM production per chondrocyte in low cell density was much higher than that in high cell seeding density. This finding was also verified by mechanical testing and histology. In conclusion, cell seeding density that is feasible for bioprinting also appears optimal for human neocartilage formation when combined with appropriate growth and differentiation factors. Biotechnol. Bioeng. 2012;109: 23572368. (c) 2012 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据