Article
Agriculture, Dairy & Animal Science
Marie-Laure Mollichella, Violaine Mechin, Dany Royer, Patrick Pageat, Pietro Asproni
Summary: This study successfully isolated olfactory ecto-mesenchymal stem cells from cat olfactory mucosa, demonstrating their stemness features and multilineage differentiation capabilities. These cells hold promising potential for application in feline regenerative medicine.
Review
Biotechnology & Applied Microbiology
Alonso Ornelas-Gonzalez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Stem cell-based therapies have emerged as an alternative for treating various diseases, with the use of microcarrier culture techniques showing promise for expanding stem cells. However, challenges in production and medical applications remain, highlighting the need for the development of new bioprocesses to simplify stem cell cultures.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Cell Biology
Tzu-Cheng Sung, Yi-Peng Jiang, Jhe-Yu Hsu, Qing-Dong Ling, Hao Chen, Suresh S. Kumar, Yung Chang, Shih-Tien Hsu, Qingsong Ye, Akon Higuchi
Summary: This study successfully generated universal hiPSCs without gene editing and evaluated their resistance to immune reactions, demonstrating that the cells maintained stable characteristics even after continuous passages.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Review
Cell Biology
Soudeh Ghafouri-Fard, Vahid Niazi, Bashdar Mahmud Hussen, Mir Davood Omrani, Mohammad Taheri, Abbas Basiri
Summary: Extracellular vesicles play important roles in cell-cell communication, inflammation modulation, cell proliferation, and tissue regeneration, with advantages such as lower risk and easier production and storage compared to cell therapy. Particularly, exosomes derived from mesenchymal stem cells are introduced as a therapeutic option for various human diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Katarzyna Czerwiec, Malgorzata Zawrzykraj, Milena Deptula, Aneta Skoniecka, Agata Tyminska, Jacek Zielinski, Adam Kosinski, Michal Pikula
Summary: Adipose-derived mesenchymal stromal cells (AD-MSCs) have gained significant attention due to their easy availability from fat tissue and their regenerative and immunomodulatory properties. These cells show great potential in stem cell-based therapies for wound healing, orthopedic, cardiovascular, and autoimmune diseases. Numerous clinical trials have provided evidence for the effectiveness of AD-MSCs. This article presents current knowledge on AD-MSCs, their applications in pre-clinical models and clinical studies, and highlights the potential for chemically or genetically modified AD-MSCs as the next generation of stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Michal Domaniza, Marian Hluchy, Dasa Cizkova, Filip Humenik, Lucia Slovinska, Nikola Hudakova, Lubica Hornakova, Juraj Vozar, Alexandra Trbolova
Summary: This study aimed to investigate the potential of cell-based regenerative therapy for elbow joints affected by osteoarthritis. The results showed that two injections of amnion-derived mesenchymal stem cells improved joint range of motion and had an impact on synovial fluid biomarkers. Concentrations of IFN-γ, IL-6, and TNF-α decreased while IL-10 increased between day 0 and day 28 after treatment.
Article
Biotechnology & Applied Microbiology
Qiulin He, Junxin Lin, Fanghao Zhou, Dandan Cai, Yiyang Yan, Yejie Shan, Shufang Zhang, Tiefeng Li, Xudong Yao, Hongwei Ouyang
Summary: Nonuniform microstretching (NUMS), a natural phenomenon in bone tissues, promotes osteogenic differentiation and has potential positive effects on bone healing.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ruei-Hong Lin, Hsu-Tung Lee, Chun-An Yeh, Yi-Chin Yang, Chiung-Chyi Shen, Kai-Bo Chang, Bai-Shuan Liu, Hsien-Hsu Hsieh, Hui-Min David Wang, Huey-Shan Hung
Summary: The research shows that using AuNPs at concentrations of 1.25 and 2.5 ppm can enhance cell proliferation, reduce reactive oxygen species generation, and attenuate platelet/monocyte activation. AuNPs can also promote cell adhesion, increase the expression of matrix metalloproteinase (MMP-2/9), decrease cell apoptosis and the release of inflammatory cytokines. Animal model experiments demonstrate that treating with 1.25 and 2.5 ppm AuNPs exhibits better biocompatibility and endothelialization. This suggests that AuNPs can be used as a biocompatible nanomedicine for regeneration research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Emergency Medicine
Courtney Premer, Ivonne H. Schulman, Jennifer S. Jackson
Summary: MSCs show promising potential in treating acute diseases, with clinical trials demonstrating safety but inconsistent efficacy data, necessitating further research.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Riccardo Giorgino, Domenico Albano, Stefano Fusco, Giuseppe M. Peretti, Laura Mangiavini, Carmelo Messina
Summary: Osteoarthritis (OA) is a common chronic orthopedic disorder that primarily affects hips and knees. The variability in the prevalence of knee OA across studies highlights the heterogeneity of reported data worldwide. The pathogenetic mechanism of knee OA involves an inflammatory process in the synovial membrane, mediated by immune system activation and damage-associated molecular patterns (DAMPs). Mesenchymal stem cells (MSCs) offer promising therapeutic options, but unresolved issues remain regarding the optimal cell source and quantity. Further research is necessary to address these uncertainties. This review provides a comprehensive overview of the molecular mechanisms of cartilage damage in knee OA and discusses current regenerative medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Matin Chehelgerdi, Fereshteh Behdarvand Dehkordi, Mohammad Chehelgerdi, Hamidreza Kabiri, Hosein Salehian-Dehkordi, Mohammad Abdolvand, Sharareh Salmanizadeh, Mohsen Rashidi, Anoosha Niazmand, Saba Ahmadi, Sara Feizbakhshan, Saber Kabiri, Nasimeh Vatandoost, Tayebeh Ranjbarnejad
Summary: The emergence of iPSCs has revolutionized stem cell research and opened up new avenues for cancer treatment. iPSCs provide a unique platform for investigating the origin of cancer and can be used for personalized treatment and drug testing. This review article discusses the science behind iPSCs, the limitations and challenges in iPSC-based cancer therapy, ethical and social implications, and a comparative analysis with other stem cell types for cancer treatment. It also explores the applications of iPSCs in tumorigenesis and the future of iPSC research in this field.
Review
Veterinary Sciences
Hussein M. M. El-Husseiny, Eman A. A. Mady, Mahmoud A. Y. Helal, Ryou Tanaka
Summary: The use of stem cells in veterinary practice is rapidly evolving, with significant research efforts focused on both experimental animals and clinical cases. Stem cells, particularly mesenchymal stem cells (MSCs), are promising for therapeutic applications due to their immunomodulatory capabilities and ease of isolation. However, challenges remain in their clinical implementation for treating various animal diseases, driving further advancements in stem cell therapies.
VETERINARY SCIENCES
(2022)
Review
Cell Biology
Yuxuan Zhong, Xiang Li, Fanglin Wang, Shoushuai Wang, Xiaohong Wang, Xiaohong Tian, Shuling Bai, Di Miao, Jun Fan
Summary: Recent studies have shown that various exosomes can influence the adipogenic differentiation of stem cells. Exosomes play an important role in promoting tissue regeneration and provide new ideas for adipose tissue engineering research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Luigia Fonticoli, Ylenia Della Rocca, Thangavelu Soundara Rajan, Giovanna Murmura, Oriana Trubiani, Stefano Oliva, Jacopo Pizzicannella, Guya Diletta Marconi, Francesca Diomede
Summary: Gingival tissue can be collected easily and used as a source to isolate GMSCs, which possess differentiation and immunomodulatory abilities similar to MSCs. GMSCs can be expanded in vitro while maintaining their stemness properties. Furthermore, the EVs released from GMSCs have shown similar biological functions and therapeutic effects, making them a promising tool for cell-free regenerative therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Claudia C. Miranda, Michael L. Akenhead, Teresa P. Silva, Michael A. Derr, Mohan C. Vemuri, Joaquim M. S. Cabral, Tiago G. Fernandes
Summary: This study developed a scalable platform for generating large numbers of high-quality tissue-specific cells from hPSCs. The use of Gibco StemScale PSC Suspension Medium led to a significant increase in cell number over multiple passages. Additionally, successful neural induction of hiPSCs was achieved, resulting in the generation of specific brain region cell populations.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Paola Sanjuan-Alberte, Charlie Whitehead, Joshua N. Jones, Joao C. Silva, Nathan Carter, Simon Kellaway, Richard J. M. Hague, Joaquim M. S. Cabral, Frederico C. Ferreira, Lisa J. White, Frankie J. Rawson
Summary: Conductive hydrogels are promising materials for bioelectronic applications. A strategy to bioprint conductive bioinks based on dECM and carbon nanotubes is proposed. These bioinks exhibit both conductivity and the morphology of dECM fibers. Electrical stimulation enhances the contractile behavior of hPSC-CMs and leads to more mature cells with upregulated calcium handling proteins and downregulated calcium channels involved in pacemaking currents. Our strategy demonstrates the potential of manufacturing complex conductive hydrogels for actuating purposes.
Review
Biotechnology & Applied Microbiology
Miguel F. Tenreiro, Mariana A. Branco, Joao P. Cotovio, Joaquim M. S. Cabral, Tiago G. Fernandes, Maria Margarida Diogo
Summary: Human adult stem cells and patient-derived induced pluripotent stem cells are valuable tools for studying human biology, development, and disease. Organoids, which are organ-like structures created from stem cell derivatives, have revolutionized the field. However, there are still limitations such as variability, limited function, and small size. In this article, we summarize the latest technologies that aim to improve the biological fidelity and physiological relevance of organoids, and discuss their potential applications.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Andre Branco, Ana L. Tiago, Paula Laranjeira, Maria C. Carreira, Joao C. Milhano, Francisco dos Santos, Joaquim M. S. Cabral, Artur Paiva, Claudia L. da Silva, Ana Fernandes-Platzgummer
Summary: Cell and gene therapies face high production costs, but encapsulation at hypothermic temperatures has shown potential to reduce costs. Adipose tissue-derived mesenchymal stromal cells (MSC(AT)) can survive and maintain functionality when encapsulated, making encapsulation a viable competitor in transport for cell and gene therapies.
BIOENGINEERING-BASEL
(2022)
Review
Biotechnology & Applied Microbiology
William O. S. Salvador, Ines A. B. Ribeiro, Diogo E. S. Nogueira, Frederico C. Ferreira, Joaquim M. S. Cabral, Carlos A. V. Rodrigues
Summary: The field of cell and gene therapy has grown rapidly due to the benefits of using living cells as therapeutic agents. Bioprocess economic models (BEMs) are essential for guiding decision-making and optimizing production processes, particularly for cell-based therapies that are often cost-prohibitive. This review highlights the important role of BEMs in developing bioprocesses for stem cell therapy products and provides a comprehensive overview of existing BEMs.
BIOENGINEERING-BASEL
(2022)
Review
Biotechnology & Applied Microbiology
Miguel de Almeida Fuzeta, Pedro P. Goncalves, Ana Fernandes-Platzgummer, Joaquim M. S. Cabral, Nuno Bernardes, Claudia L. da Silva
Summary: This article reviews the biology of extracellular vesicles (EVs), including their role in intercellular communication, pathophysiological processes, therapeutic potential, and the application of bioengineering strategies.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Ana Fernandes-Platzgummer, Raquel Cunha, Sara Morini, Marta Carvalho, Juan Moreno-Cid, Carmen Garcia, Joaquim M. S. Cabral, Claudia L. da Silva
Summary: This study successfully establishes a serum-/xenogeneic-free microcarrier-based culture system for the cultivation of bone marrow-derived MSC and production of MSC-EVs. Large numbers of cells and EVs were obtained through static culture and continuous perfusion. The size and concentration of EVs were estimated using nanoparticle tracking analysis.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Mariana A. Branco, Tiago C. Nunes, Joaquim M. S. Cabral, Maria Margarida Diogo
Summary: It is now acknowledged that drug use during pregnancy is necessary to prevent or treat illnesses, but there is often a lack of information on teratogenic risk in humans. Animal models have been the main source of data, but their limitations in predicting human outcomes have led to misidentification of teratogenicity. The development of humanized in vitro models, particularly those that replicate important early developmental stages, can overcome these limitations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Biochemistry & Molecular Biology
Frederico Barbosa, Fabio F. F. Garrudo, Ana C. Marques, Joaquim M. S. Cabral, Jorge Morgado, Frederico Castelo Ferreira, Joao C. Silva
Summary: This study aimed to develop electroconductive PAN/PEDOT:PSS nanofibers via electrospinning, which can mimic the fibrous extracellular matrix (ECM) of natural tissue and provide a platform for the delivery of exogenous electrical stimulation (ES). The resulting nanofibers were functionalized with apatite-like structures to imitate the inorganic phase of the bone ECM. The mineralized electroconductive nanofibers demonstrated improved biological performance, including enhanced proliferation of cells and up-regulated expression levels of bone marker genes, making them potential electroactive biomimetic scaffolds for bone defect repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Claudia C. Miranda, Mariana Ramalho Gomes, Mariana Moco, Joaquim M. S. Cabral, Frederico Castelo Ferreira, Paola Sanjuan-Alberte
Summary: Chronic kidney disease is a global deadly disease and current treatment methods are insufficient. Engineering kidney tissues from induced pluripotent stem cells (iPSCs) could provide a solution, but implementation is challenging due to the complexity of mature kidneys. Electrospinning, a technique using synthetic and natural polymers to create fibers, shows promise in kidney tissue engineering. However, further research is needed to advance the applications of electrospinning in combination with other manufacturing methods.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Pedro M. Neto, Diogo E. S. Nogueira, Yas Hashimura, Sunghoon Jung, Bruno Pedras, Mario N. Berberan-Santos, Tiago Palmeira, Brian Lee, Joaquim M. S. Cabral, Vitor Geraldes, Carlos A. Rodrigues
Summary: In this study, oxygen transport and hydrodynamic flow in the PBS Vertical-Wheel MINI 0.1 bioreactor were characterized using experimental data and computational fluid dynamics simulations. The results provide new insights into oxygen mass transfer and hydrodynamics, and offer a computational model for further studies.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Diana M. C. Marques, Joao C. Silva, Ana Paula Serro, Joaquim M. S. Cabral, Paola Sanjuan-Alberte, Frederico C. Ferreira
Summary: In this study, novel bioinks based on kappa-carrageenan were successfully formulated and used to fabricate 3D scaffolds. The ink formulation and printing parameters were optimized, enabling the manufacturing of complex structures. Mechanical tests and cell viability assays demonstrated the excellent performance of this material, indicating its potential applications in tissue engineering and cellular agriculture.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Brian Lee, Sunghoon Jung, Yas Hashimura, Maximilian Lee, Breanna S. Borys, Tiffany Dang, Michael S. Kallos, Carlos A. Rodrigues, Teresa P. Silva, Joaquim M. S. Cabral
Summary: This manuscript discusses the challenges of manufacturing therapeutic cells derived from pluripotent stem cells using single-use bioreactors and presents potential solutions.
BIOENGINEERING-BASEL
(2022)