Integrated Analysis of the Global Transcriptional Response to α-Amylase Over-Production by Aspergillus oryzae

The filamentous fungus Aspergillus oryzae is an important microbial cell factory for industrial production of many enzymes, such as alpha-amylase. In order to optimize the industrial enzyme production process, there is a need to understand fundamental processes underlying enzyme production, here under how enzyme production links to metabolism through global regulation. Through a genome-scale metabolic network for integrated analysis of transcriptome data and flux calculation, we identified key players (genes, enzymes, proteins, and metabolites) involved in the processes of enzyme synthesis and secretion, nucleotide metabolism, and amino acid metabolism that can be the potential targets for improving industrial enzyme production. Biotechnol. Bioeng. 2011;108: 1130-1139. (C) 2010 Wiley Periodicals, Inc.