期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 106, 期 5, 页码 841-844出版社
WILEY
DOI: 10.1002/bit.22739
关键词
non-ribosomal peptide synthetase; yeast; COM domain; secondary metabolism
资金
- Chalmers Foundation
- Knut and Alice Wallenberg Foundation
- European Union
Saccharomyces cerevisiae has in several cases been proven to be a suitable host for the production of natural products and was recently exploited for the production of non-ribosomal peptides. Synthesis of non-ribosomal peptides (NRPs) is mediated by NRP synthetases (NRPSs), modular enzymes, which are often organized in enzyme complexes. In these complexes, partner NRPSs interact via communication-mediating domains (COM domains). In order to test whether functional interaction between separate NRPS modules is possible in yeast we constructed a yeast strain expressing two modules with compatible COM domains from two plasmids. Successful production as well as secretion of the expected dipeptide was detected. This opens the possibility of using yeast as a eukaryotic platform for fast assessment of new module combinations for the development of novel NRP compounds. Biotechnol. Bioeng. 2010;106: 841-844. (C) 2010 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据