Article
Multidisciplinary Sciences
Ding Wang, Jing Zhu, Na Li, Hongyang Lu, Yun Gao, Lei Zhuang, Zhongjian Chen, Weimin Mao
Summary: This study analyzed the plasma metabolic profile of patients with malignant mesothelioma and identified differential metabolites and disrupted metabolic pathways. Five potential plasma biomarkers were identified, which could be critical for the diagnosis of malignant mesothelioma.
Article
Public, Environmental & Occupational Health
Xuezhi Wang, Zhuhua Wu, Jincheng Zeng, Yuchuan Zhao, Chenchen Zhang, Meiling Yu, Wei Wang, Xunxun Chen, Liang Chen, Jiawen Wang, Liuyue Xu, Jie Zhou, Qiuchan Tan, Wenjing Wei, Yanxia Li
Summary: This study characterized the changes in serum metabolic profiles caused by Mtb infection and standard anti-TB treatment. Seven metabolites were identified to have significantly higher levels in LTBI and untreated PTB patients compared to uninfected healthy controls. Akeboside Ste and sulfolithocholic acid were significantly decreased in PTB patients after treatment. The differential metabolites were mainly involved in glutathione metabolism, ascorbate and aldarate metabolism, and porphyrin and chlorophyll metabolism.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Microbiology
Estefane da C. Nunes, Ana M. B. de Filippis, Taiane do E. S. Pereira, Nieli R. da C. Faria, Alvaro Salgado, Cleiton S. Santos, Teresa C. P. X. Carvalho, Juan Calcagno, Flivia L. L. Chalhoub, David Brown, Marta Giovanetti, Luiz C. J. Alcantara, Fernanda K. Barreto, Isadora C. de Siqueira, Gisele A. B. Canuto
Summary: Zika virus infection can impact neural development and cellular metabolism, with significant alterations in the metabolic profiles of infected newborns observed in this study. This research provides insights into relevant metabolic processes in patients with ZIKV and microcephaly.
Article
Agronomy
Ni Zhang, Weiwen Tan, Guimei Luo, Tianyi Pu, Jinqiu Wang, Xianhu Zhang, Yuehua Song
Summary: Camellia tetracocca, a unique tea species in southwest China, is frequently attacked by the pest Empoasca onukii. The metabolic mechanisms of C. tetracocca's defense against this pest are still unclear. Through comparative analysis using GC-MS and LC-MS, differential metabolites were identified, showing that accumulation of specialized metabolites may contribute to the resistance of C. tetracocca against E. onukii. These specialized metabolites have the potential to be used as biocontrol agents against the pest.
Article
Chemistry, Applied
Maria Perez-Jimenez, Emma Sherman, Maria Angeles Pozo-Bayon, Carolina Munoz-Gonzalez, Farhana R. Pinu
Summary: This study employed an untargeted volatile profiling approach and a spit off-odorant measurement procedure to investigate the fate of aroma compounds in the mouth. The results show that oral processing and intrinsic biological variables influence the overall volatile composition, and age and gender specific differences exist in wine volatile composition due to oral processing.
Article
Microbiology
Shu Wu, Kun Yang, Yuhang Hong, Yanju Gong, Jiajia Ni, Ni Yang, Weijun Ding
Summary: This study investigates the antibacterial mechanism of berberine hydrochloride (BBR) using metabolomics techniques. It is found that BBR reduces the antioxidant capacity of Staphylococcus aureus, inhibits cell wall synthesis, and disrupts the shikimate pathway and aromatic amino acid synthesis. These findings provide new insights into the antibacterial properties of BBR and suggest potential directions for further research.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Lei He, Qian Hu, Jiukai Zhang, Ranran Xing, Yongsheng Zhao, Ning Yu, Ying Chen
Summary: This study comprehensively investigated the metabolite variability of black soybeans from different geographical origins in China. The results showed that black soybeans from Gansu had higher amounts of procyanidins, epicatechin, malonylated isoflavones, and beta-pinene. Guangxi black soybeans had higher amounts of linoleic acid and its oxidation products, while Xinjiang and Yunnan black soybeans contained higher levels of delphinidin-derived anthocyanins, gamma-glutamyl peptides, and aromatic hydrocarbons. The study also identified characteristic metabolites in black soybeans from other geographical origins.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Pharmacology & Pharmacy
Ali Raza, Moatter Zehra, Muhammad Ramzan, Amna Jabbar Siddiqui, Azra Akbar, Ayaz Ahmed, Syed Ghulam Musharraf
Summary: To understand the development of antibiotic resistance in Klebsiella pneumoniae, this study conducted metabolomics analysis on sensitive and gentamicin-resistant strains. The results showed that increasing gentamicin concentration led to morphological changes in K. pneumoniae colonies, and strains grown at the highest concentration were considered resistant. Metabolomic analysis identified several regulated metabolites associated with resistance development, which could potentially be used for rapid detection of gentamicin-resistant clinical strains.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Microbiology
Emilia Galdiero, Maria Michela Salvatore, Angela Maione, Federica Carraturo, Stefania Galdiero, Annarita Falanga, Anna Andolfi, Francesco Salvatore, Marco Guida
Summary: The study highlights the therapeutic potential of peptide WMR-K in inhibiting biofilm formation and eradicating biofilms in multi-species microbial systems, with significant inhibitory effects on Klebsiella pneumoniae and Candida albicans. The metabolomic profile of the treated culture showed alterations in primary metabolic pathways, indicating potential mechanisms of action for the peptide.
Review
Chemistry, Analytical
Leticia Lacalle-Bergeron, David Izquierdo-Sandoval, Juan V. Sancho, Francisco J. Lopez, Felix Hernandez, Tania Portoles
Summary: There is a growing demand to increase knowledge about the quality of food and its relationship with health and disease. Untargeted metabolomics approaches are powerful tools for exploring metabolic changes, with potential in the food field. Advanced analytical techniques like high resolution mass spectrometry are necessary for covering a wide range of metabolites in complex samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Rajender Kumar, Dinesh Kumar
Summary: Allium is widely consumed as a spice around the world. This study compared the metabolome and antioxidant activity of different tissues of Allium cepa, A. sativum, and A. semenovii. The results showed that A. cepa and A. semenovii had higher antioxidant activity and polyphenol content compared to A. sativum. Additionally, various metabolites including polyphenols and sulfur containing compounds were identified. This study highlights the potential of A. semenovii for utilization in food and nutraceuticals.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Leticia Lacalle-Bergeron, Rafael Goterris-Cerisuelo, Tania Portoles, Joaquin Beltran, Juan Vicente Sancho, Cinta Navarro-Moreno, Fernando Martinez-Garcia
Summary: This study identified 11 mouse pup volatiles potentially involved in maternal care induction in adult females using dynamic headspace and GC-MS techniques. The comparison between neonatal and elder pups revealed specific compounds and enrichment of palmitic acid in older pups. The results demonstrated the potential of the new sampling procedure combined with GC-MS for volatile pheromone identification in mammals.
Review
Chemistry, Analytical
Giacomo Baccolo, Beatriz Quintanilla-Casas, Stefania Vichi, Dillen Augustijn, Rasmus Bro
Summary: Gas chromatography-mass spectrometry (GC-MS) is crucial in contemporary untargeted chemical analysis, but software uncertainty can lead to reproducibility issues. By utilizing tensor-based modeling and machine learning, a fully automated method was developed to convert GC-MS data into peak tables, improving analysis efficiency and reproducibility in comparison to current methods.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Sampson D. Umoh, Gomotsang Bojase, Ishmael B. Masesane, Runner T. Majinda, Kwenga F. Sichilongo
Summary: This study identified a total of 97 phytoconstituents, including 17 bioactive compounds, in Combretum platypetalum for the first time. The results suggest that the extraction methods used can influence the concentration of compounds. These findings support the potential applications of C. platypetalum in drug discovery.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Ruba Al-Nemi, Arwa A. Makki, Khaled Sawalha, Dina Hajjar, Mariusz Jaremko
Summary: This study investigated the chemical profiles and antioxidant capacities of different solvent extracts of Ephedra foeminea. The results showed that the methanolic extract had the highest antioxidant activity, which may be attributed to its abundant bioactive compounds.
Article
Nutrition & Dietetics
Carina I. Mack, Christoph H. Weinert, Bjoern Egert, Paola G. Ferrario, Achim Bub, Ingrid Hoffmann, Bernhard Watzl, Hannelore Daniel, Sabine E. Kulling
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2018)
Article
Nutrition & Dietetics
M. Armbruster, M. Rist, S. Seifert, L. Frommherz, C. Weinert, C. Mack, A. Roth, B. Merz, D. Bunzel, R. Krueger, S. Kulling, B. Watzl, A. Bub
EUROPEAN JOURNAL OF NUTRITION
(2019)
Review
Food Science & Technology
Marynka M. Ulaszewska, Christoph H. Weinert, Alessia Trimigno, Reto Portmann, Cristina Andres Lacueva, Rene Badertscher, Lorraine Brennan, Carl Brunius, Achim Bub, Francesco Capozzi, Marta Cialie Rosso, Chiara E. Cordero, Hannelore Daniel, Stephanie Durand, Bjoern Egert, Paola G. Ferrario, Edith J. M. Feskens, Pietro Franceschi, Mar Garcia-Aloy, Franck Giacomoni, Pieter Giesbertz, Raul Gonzalez-Dominguez, Kati Hanhineva, Lieselot Y. Hemeryck, Joachim Kopka, Sabine E. Kulling, Rafael Llorach, Claudine Manach, Fulvio Mattivi, Carole Migne, Linda H. Munger, Beate Ott, Gianfranco Picone, Gregory Pimentel, Estelle Pujos-Guillot, Samantha Riccadonna, Manuela J. Rist, Caroline Rombouts, Josep Rubert, Thomas Skurk, Pedapati S. C. Sri Harsha, Lieven Van Meulebroek, Lynn Vanhaecke, Rosa Vazquez-Fresno, David Wishart, Guy Vergeres
MOLECULAR NUTRITION & FOOD RESEARCH
(2019)
Article
Food Science & Technology
Carina I. Mack, Bjoern Egert, Erica Liberto, Christoph H. Weinert, Achim Bub, Ingrid Hoffmann, Carlo Bicchi, Sabine E. Kulling, Chiara Cordero
MOLECULAR NUTRITION & FOOD RESEARCH
(2019)
Article
Nutrition & Dietetics
Natalia Vazquez-Manjarrez, Christoph H. Weinert, Maria M. Ulaszewska, Carina I. Mack, Pierre Micheau, Melanie Petera, Stephanie Durand, Estelle Pujos-Guillot, Bjorn Egert, Fulvio Mattivi, Achim Bub, Lars Ove Dragsted, Sabine E. Kulling, Claudine Manach
JOURNAL OF NUTRITION
(2019)
Article
Food Science & Technology
Ilka Guenther, Gerald Rimbach, Carina Mack, Christoph H. Weinert, Nicolas Danylec, Kai Lueersen, Marc Birringer, Franz Bracher, Sebastian T. Soukup, Sabine E. Kulling, Kathrin Pallauf
MOLECULAR NUTRITION & FOOD RESEARCH
(2020)
Article
Plant Sciences
M. L. Romo-Perez, C. H. Weinert, M. Haeussler, B. Egert, M. A. Frechen, B. Trierweiler, S. E. Kulling, C. Zoerb
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2020)
Article
Food Science & Technology
Carina Mack, Paola G. Ferrario, Christoph H. Weinert, Bjoern Egert, Anja S. Hoefle, Yu-Mi Lee, Thomas Skurk, Sabine E. Kulling, Hannelore Daniel
MOLECULAR NUTRITION & FOOD RESEARCH
(2020)
Article
Food Science & Technology
Mar Garcia-Aloy, Marynka Ulaszewska, Pietro Franceschi, Sheila Estruel-Amades, Christoph H. Weinert, Alba Tor-Roca, Mireia Urpi-Sarda, Fulvio Mattivi, Cristina Andres-Lacueva
MOLECULAR NUTRITION & FOOD RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Marta Cialie Rosso, Federico Stilo, Carlo Bicchi, Melanie Charron, Ginevra Rosso, Roberto Menta, Stephen E. Reichenbach, Christoph H. Weinert, Carina I. Mack, Sabine E. Kulling, Chiara Cordero
Summary: This study focused on the detectable metabolome changes in high-quality raw hazelnuts and identified discriminant components such as amino acids, organic acids, sugars, and polyols that play important roles in distinguishing hazelnut cultivars and the impact of roasting. Roasting significantly decreased levels of certain amino acids, organic acids, and sugars in hazelnuts, leading to changes in metabolite patterns.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Manuela Peukert, Sebastian Zimmermann, Bjoern Egert, Christoph H. Weinert, Thomas Schwarzmann, Dagmar A. Brueggemann
Summary: The study found pronounced gender differences between gilts and boars, while genotype-related differences were less significant. Additionally, the metabolic dimorphism between gilts and boars was found to be genotype-dependent, and vice versa metabolic differences between genotypes were found to be gender-dependent. Moreover, in terms of nitrogen and carbon utilization, boars showed a stronger nitrogen recycling and increased energy metabolism, whereas gilts tended to excrete more nitrogen and channel the remaining carbon into lipogenesis.
Article
Biochemistry & Molecular Biology
Sina Kistner, Maik Doering, Ralf Krueger, Manuela J. Rist, Christoph H. Weinert, Diana Bunzel, Benedikt Merz, Katrin Radloff, Rainer Neumann, Sascha Haertel, Achim Bub
Summary: This study revealed sex-related differences in plasma metabolite patterns associated with cardiorespiratory fitness, and confirmed known associations between cardiorespiratory fitness and risk factors for cardiometabolic diseases in metabolically healthy individuals. The findings indicate that covariates such as sex and body composition need to be considered when studying blood metabolic markers related to cardiorespiratory fitness.
Article
Plant Sciences
M. L. Romo-Perez, C. H. Weinert, B. Egert, B. L. Franzisky, S. E. Kulling, C. Zoerb
Summary: The study found that onion plants have the ability to exclude Na+ after moderate Na2SO4 treatment, foliar application does not lead to Na+ accumulation in the bulbs, while soil application results in Na+ accumulation in the leaves and bulbs, with no significant impact on the metabolite profile of onions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Caroline Bender, Dominic Stoll, Melanie Huch, Christoph Weinert, Andreas Doetsch, Hannah Draeger, Judith Keller, Sabine Kulling, Mirko Bunzel
Summary: This study investigated the fermentation kinetics and structural differences of six commercial pectins from citrus, apple, and sugar beet. The results showed that different structural units of pectins might undergo fermentation in different sections of the colon, potentially affecting their nutritional properties.
CARBOHYDRATE POLYMERS
(2023)
Article
Plant Sciences
Samia Daldoul, Mahmoud Gargouri, Christoph Weinert, Ali Jarrar, Bjoern Egert, Ahmed Mliki, Peter Nick
Summary: This study compared the physiological and metabolic responses of a salt-tolerant accession of Vitis sylvestris, Tebaba, from Tunisia, with the widely used 1103 Paulsen rootstock in the Mediterranean. It was found that Tebaba can cope with salinity through robust redox homeostasis, rechanneling metabolic pathways towards antioxidants and compatible osmolytes, and buffering photosynthesis to avoid cell-wall breakdown. It is suggested that introgression of Tebaba into commercial grapevine varieties is preferred over using Tebaba as a rootstock for improving salt tolerance.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)