Article
Chemistry, Multidisciplinary
Jiranuwat Sapudom, Shaza Karaman, Brian Chesney Quartey, Walaa Kamal Eldin Mohamed, Nick Mahtani, Anna Garcia-Sabate, Jeremy Teo
Summary: This article develops a simple approach to reconstitute homogeneous 3D collagen matrices with adjustable degree of fibril alignment using 3D printed inclined surfaces. It is found that the elastic modulus of collagen matrices is enhanced with an increase in the alignment degree. The reconstituted matrices are used to study fibroblast behavior and reveal the impact of collagen fibril organization on fibroblast differentiation.
Review
Biochemistry & Molecular Biology
Ewa Bednarczyk
Summary: This paper discusses the methods of cell cultures for investigating the mechanical causes, therapeutic solutions, and preventive methods of osteoarthritis. It focuses on the cultivation of cartilage cells to recreate the mechanical loads that contribute to the development of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Thunwa Binlateh, Pilaiwanwadee Hutamekalin, Jirawat Yongsawatdigul, Montarop Yamabhai, Paiboon Jitprasertwong
Summary: Although collagen and chitosan have been proven to have potential for tissue repair, their combination effects remain unclear. This study examined the impact of single collagen, chitosan, and their mixture on fibroblasts and endothelial cells. The results showed that either collagen or chitosan stimulation significantly promoted fibroblast responses and endothelial cell proliferation and migration, with collagen providing a stronger effect. A 1:10 mixture of collagen and chitosan further enhanced these effects, suggesting their potential use as biomaterials for tissue repair.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Chemistry, Physical
Serena Bianchi, Sara Bernardi, Davide Simeone, Diana Torge, Guido Macchiarelli, Enrico Marchetti
Summary: This study investigated the proliferation and attachment abilities of human periodontal ligament fibroblasts on two bovine pericardium membranes with different thicknesses. The results showed similar cell behavior on both membranes, with cells migrating and adhering to the membrane's surface.
Article
Biochemistry & Molecular Biology
Yichen Xu, Huabo Zheng, Pakhwan Nilcham, Octavian Bucur, Felix Vogt, Ioana Slabu, Elisa Anamaria Liehn, Mihaela Rusu
Summary: Extracellular collagen remodeling is a central mechanism in maintaining the coherence of myocardium after myocardial infarction. Vitamin C has the potential to modulate collagen fibrillogenesis and improve heart tissue healing. It affects cellular metabolism and the production of reactive oxygen species, while promoting collagen synthesis and tissue healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Putu Ustriyana, Rui He, Sudarshan Srirangapatanam, Jasper Chang, Sheeler T. Arman, Sukhmandeep Sidhu, Bo Wang, Misun Kang, Sunita P. Ho
Summary: The study on mice chewing hard and soft food during experimental tooth movement showed that chewing soft food led to wider periodontal ligament space and increased alveolar socket roughness, with implications for regulating chewing loads post-treatment for effective periodontal complex regeneration.
JOURNAL OF PERIODONTAL RESEARCH
(2022)
Article
Cell Biology
Liuhanghang Cheng, Xiaoxuan Lei, Zengjun Yang, Yanan Kong, Pengcheng Xu, Shiya Peng, Jue Wang, Cheng Chen, Yunqing Dong, Xiaohong Hu, Xiaorong Zhang, Tymour Forouzanfar, Gang Wu, Xiaobing Fu
Summary: Histatin 1 has been found to accelerate wound healing and improve the mechanical properties of healed skin by promoting the function of fibroblasts. In vitro experiments showed that fibroblasts stimulated by Hst 1 exhibited enhanced migration ability and transformation into myofibroblasts, leading to improved contraction and collagen secretion functions. Additionally, the study identified activation of the mTOR signaling pathway by Hst 1 in fibroblasts.
CELL PROLIFERATION
(2021)
Review
Engineering, Biomedical
Emily M. Carvalho, Sanjay Kumar
Summary: Biomaterials play a crucial role in studying and controlling cell behaviors, and their viscoelastic properties greatly influence cell behavior. This review discusses the advances in viscoelastic materials for cell engineering, focusing on the mechanisms of collagen, alginate, hyaluronic acid, and fibrous materials in regulating cell behavior.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Carla Souza, Cristiano Ceron Jayme, Nayara Rezende, Antonio Claudio Tedesco
Summary: The combined therapy of PBM and AlClPc/NE treatment can modify fibroblast signaling response in a 3D environment, promoting collagen synthesis, ROS production, MMP-9 secretion, actin network proliferation, and myofibroblastic differentiation.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Engineering, Biomedical
Diana M. Darvish
Summary: Collagen type I is a frequently used biomaterial in tissue engineering and regenerative medicine, showcasing advantages such as mimicking the structure of native extracellular matrix. However, controversies surround the methods for collagen fibril formation, such as temperature settings and extraction methods. This review aims to summarize important studies on in vitro collagen fibril formation and address these issues.
MATERIALS TODAY BIO
(2022)
Article
Biochemistry & Molecular Biology
Anis Hanna, Arti V. Shinde, Ruoshui Li, Linda Alex, Claudio Humeres, Prasanth Balasubramanian, Nikolaos G. Frangogiannis
Summary: Tissue injury leads to alterations in collagen network, with specific patterns of collagen denaturation observed during different phases of infarct healing. MT1-MMP plays a significant role in pericellular collagen denaturation, while mechanical tension can induce peripheral collagen denaturation. Chronic collagen denaturation may contribute to post-infarction heart failure.
Article
Biochemistry & Molecular Biology
Aya Tentaku, Shusaku Kurisu, Kurumi Sejima, Toshiki Nagao, Akira Takahashi, Shigenobu Yonemura
Summary: The basement membrane (BM) is a crucial extracellular matrix that maintains tissue integrity and function. The mechanisms of how stroma-derived macromolecules, such as collagen IV, are transported and assembled during BM formation remained unclear. This study established an in vitro co-culture model and found that fibroblasts proximal to epithelial cells exclusively contribute to collagen IV assembly.
Article
Cell Biology
Aleksandra Maksimova, Ekaterina Shevela, Lyudmila Sakhno, Marina Tikhonova, Aleksandr Ostanin, Elena Chernykh
Summary: Apoptosis and removal of dead cells are important for wound healing. Macrophages play a role in phagocytosis and resolving inflammation. However, their involvement in fibrogenesis and its mechanisms are still unclear.
Article
Dermatology
Ewa Markiewicz, Jaimie Jerome, Thomas Mammone, Olusola C. Idowu
Summary: This study aimed to define the potential targets for screening and validation of anti-glycation activity of novel cosmetic candidates from natural products and to provide a plausible mechanism for their anti-aging potential based on 3D skin models. The findings suggest that glycation can result in age-related alterations in the structural and cellular organizations of the skin, which can be restored by Resveratrol derivatives.
CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY
(2022)
Article
Biology
E. E. Van Haaften, S. Quicken, W. Huberts, C. V. C. Bouten, N. A. Kurniawan
Summary: Researchers demonstrate a numerical-experimental approach to reconstruct the graft-host response and investigate mechanoregulation in dialysis grafts. They find that oscillatory shear stress, in the presence of cyclic strain, promotes neotissue development by influencing vascular cell secretion and collagen network formation, providing a potential method to inhibit neointimal hyperplasia in grafts.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chiara Emma Campiglio, Marina Figliuzzi, Sara Silvani, Matteo Tironi, Sara Conti, Federica Boschetti, Andrea Remuzzi
Summary: The study demonstrated that different culture substrate materials can influence the morphology and functionality of lung epithelial adenocarcinoma cells. The findings indicated that cells grown on Elastosil(R) Film maintained an alveolar Type II cell morphology, while those on Thermanox(TM) Coverslips adopted an alveolar Type I phenotype. This suggests that the choice of substrate material can impact cell phenotype and function.
Article
Engineering, Biomedical
Stefania Scialla, Fabiana Gullotta, Daniela Izzo, Barbara Palazzo, Francesca Scalera, Ivan Martin, Alessandro Sannino, Francesca Gervaso
Summary: Articular cartilage degeneration is a challenging issue with limited repair capabilities. Tissue engineering strategies using appropriate scaffolds for chondrogenic differentiation of stem cells can be a promising solution. This study investigated the influence of two genipin crosslinking routes on collagen-based scaffolds and found that bulk crosslinking with genipin resulted in a more uniform and homogeneous deposition of chondral-like matrix.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Engineering, Chemical
Paola Nitti, Barbara Palazzo, Nunzia Gallo, Francesca Scalera, Alessandro Sannino, Francesca Gervaso
Summary: This paper introduces a bilayer PLGA membrane prepared by coupling different techniques for Guided Tissue Regeneration. Analysis shows that the membrane has suitable properties from morphological, physicochemical, and mechanical perspectives, making it ideal for repairing damaged periodontal tissue.
POLYMER ENGINEERING AND SCIENCE
(2022)
Editorial Material
Chemistry, Multidisciplinary
Federica Boschetti
APPLIED SCIENCES-BASEL
(2022)
Article
Multidisciplinary Sciences
Xi Wang, Kai Wang, Ming Yu, Diana Velluto, Xuechong Hong, Bo Wang, Alan Chiu, Juan M. Melero-Martin, Alice A. Tomei, Minglin Ma
Summary: Engineered MSCs can improve the outcome of islet transplantation by reducing systemic immunosuppression and promoting immunomodulation. Immunophenotyping revealed reduced infiltration of T effector cells and increased infiltration of T regulatory cells in the co-transplanted islets with MSCs.
Article
Chemistry, Analytical
Margherita Pizzi, Francesco De Gaetano, Marco Ferroni, Federica Boschetti, Massimiliano Annoni
Summary: The mechanisms of microdrilling of pure Mg material for intraocular drug delivery device prototyping were experimentally studied. The experiments were conducted with different microdrills and cutting parameters, and the results showed that the burr height is not uniform along the circumference of the holes and the roughness of the inner surface increases with higher feed rate.
Article
Engineering, Manufacturing
Alessio Bucciarelli, Xenia Paolelli, Eleonora De Vitis, Nora Selicato, Francesca Gervaso, Giuseppe Gigli, Lorenzo Moroni, Alessandro Polini
Summary: In this study, the authors propose a method for fabricating microfluidic devices using digital light processing (DLP) technology and commercially available biocompatible resin. Through the use of a statistical methodology called Design of Experiment (DoE), they were able to optimize the process and achieve small features with high aspect ratio. The authors demonstrated that DLP printing is not only suitable for developing microfluidic devices, but also capable of reproducing small features rapidly. The fabricated devices exhibited good transparency and mechanical strength.
ADDITIVE MANUFACTURING
(2022)
Article
Green & Sustainable Science & Technology
F. Scalera, S. I. A. Pereira, A. Bucciarelli, D. M. Tobaldi, A. Quarta, F. Gervaso, P. M. L. Castro, A. Polini, C. Piccirillo
Summary: Chitosan (Cs) and hydroxyapatite (HA) 3D scaffolds/composites were prepared using a sustainable process, with HA obtained from cork-derived CaCO3 acting as a template agent. HA@Cs composites were formed by in situ HA formation in a Cs solution. The CsHA_72 composite exhibited improved pore structure, smaller and more circular pores, excellent biocompatibility, and good cell proliferation, making it a suitable biomaterial.
MATERIALS TODAY SUSTAINABILITY
(2023)
Review
Polymer Science
Giulia Morello, Gianvito De Iaco, Giuseppe Gigli, Alessandro Polini, Francesca Gervaso
Summary: Hydrogels are biomaterials that can act as a scaffold for cells to organize themselves spatially, similar to what occurs in vivo. They are essential for the development of 3D systems and can recreate the cellular microenvironment, making them ideal for tissue engineering and 3D in vitro modeling.
Meeting Abstract
Cell & Tissue Engineering
Giulia Morello, Alessandra Quarta, Antonio Gaballo, Alessandro Polini, Giuseppe Gigli, Lorenzo Moroni, Francesca Gervaso
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Francesca Scalera, Anna Grazia Monteduro, Giuseppe Maruccio, Francesca Gervaso, Clara Piccirillo
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Antonella Stanzione, Alessandro Polini, Alessandro Romano, Velia La Pesa, Pamela Mozetic, Lorenzo Moroni, Giuseppe Gigli, Francesca Gervaso
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Eleonora De Vitis, Velia La Pesa, Francesca Gervaso, Alessandro Romano, Angelo Quattrini, Giuseppe Gigli, Lorenzo Moroni, Alessandro Polini
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Vita Guarino, Elisabetta Perrone, Alessandra Zizzari, Monica Bianco, Antonella Stanzione, Francesca Gervaso, Giuseppe Gigli, Lorenzo Moroni, Valentina Arima
TISSUE ENGINEERING PART A
(2022)
Article
Biotechnology & Applied Microbiology
Lucia Aidos, Silvia Clotilde Modina, Valentina Rafaela Herrera Millar, Giuseppe Maria Peretti, Laura Mangiavini, Marco Ferroni, Federica Boschetti, Alessia Di Giancamillo
Summary: This study aimed to analyze the morphological, structural, biochemical, and mechanical changes of the Extracellular Matrix (ECM) during meniscus development. The study collected fully developed menisci (FD), partially developed menisci (PD), and undeveloped menisci (ND) from pigs of different ages. The cellularity and glycosaminoglycans (GAGs) deposition were evaluated, along with the analysis of Collagen 1 and aggrecan through immunohistochemistry and Western blot. The results showed clear morphological and biomechanical changes in the swine meniscus, indicating the maturation and specialization of the meniscus during growth.
BIOENGINEERING-BASEL
(2022)