Review
Plant Sciences
Paul H. Goodwin, Madison A. Best
Summary: Ginsenosides have been widely studied for their medicinal benefits and their role in ginseng plant biology. They play a crucial role in ginseng's defense against biotic stresses, providing antimicrobial, antifeedant, and allelopathic activities. Ginsenosides also have roles in ginseng development and abiotic stress tolerance.
Article
Biochemical Research Methods
Mengying Shuai, Yang Yang, Fuqing Bai, Lijun Cao, Ruyan Hou, Chuanyi Peng, Huimei Cai
Summary: This study used metabolomic methods to analyze the chemical composition of American ginseng samples from different regions. By combining linear discriminant analysis and random forest modeling, the study successfully distinguished the origin of American ginseng and discriminated between samples with and without protected designation of origin.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Prasad Kesanakurti, Subramanyam Ragupathy, Adam C. Faller, Dhivya Shanmughanandhan, Francesco Buongiorno, Isabella Della Noce, Zhengfei Lu, Yanjun Zhang, Steven G. Newmaster
Summary: Authentication of Panax ginseng and Panax quinquefolius herbal dietary supplements is crucial in mitigating adulteration and substitution issues in the international ginseng supply chain. Species-specific hydrolysis probe qPCR assays have been developed and validated, demonstrating high specificity and reliability for the identification of target species in raw materials.
Review
Integrative & Complementary Medicine
Niu Xue-Ni, Luo Wen, Lv Chong-Ning, Lu Jin-Cai
Summary: This article introduces the characteristics, sources, and semi-synthetic methods of OT-type ginsenosides, emphasizing their importance in biological activity research.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Pharmacology & Pharmacy
Rong Dong, Yougang Zhang, Shanjun Chen, Huan Wang, Kaiqing Hu, Huanxin Zhao, Qingping Tian, Kewu Zeng, Songsong Wang, Liwen Han
Summary: This study rapidly identified the pharmacodynamic markers (P-markers) against heart failure in American ginseng (AG) using the zebrafish model and metabolomics technology. These P-markers may provide new reference standards for quality control and new drug development of AG.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Valentina Cecarini, Massimiliano Cuccioloni, Chunmei Gong, Ziqi Liu, Laura Bonfili, Mauro Angeletti, Simone Angeloni, Laura Alessandroni, Gianni Sagratini, Huimin Liu, Anna Maria Eleuteri
Summary: A study investigated the effect of a Panax ginseng extract and its major components on cholesterol homeostasis. It was found that these components reduced cholesterol levels by modulating signaling pathways, stimulated cholesterol excretion and conversion, and inhibited HMGCR activity via a statin-like mechanism.
Article
Horticulture
Xiaoxue Fang, Xinteng Zhou, Yuming Wang, Yuqi Wang, Huaying Wang
Summary: This study analyzed the ginsenosides content and transcriptome data of different ginseng cultivars, and found that the total ginsenosides content was higher in leaves compared to roots and stems. Furthermore, the content of total ginsenosides and individual monomers varied significantly among cultivars. The study also identified differentially alternative splicing genes (DASGs) and discovered that transcription factors (TFs) were highly correlated with the expression of ginsenosides synthesis genes. These findings provide insights into the mechanisms of ginsenoside synthesis and offer potential for the development of natural medicines.
SCIENTIA HORTICULTURAE
(2023)
Article
Pharmacology & Pharmacy
Xilan Tang, Xiaohong Tracey Gan, Chian Ju Jong, Venkatesh Rajapurohitam, Morris Karmazyn
Summary: This study found that North American ginseng can alleviate the effects of angiotensin II on cardiac hypertrophy and dysfunction, potentially through modulation of fatty acid and glucose oxidation.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2021)
Review
Plant Sciences
Zubair Ahmed Ratan, Mohammad Faisal Haidere, Yo Han Hong, Sang Hee Park, Jeong-Oog Lee, Jongsung Lee, Jae Youl Cho
Summary: Ginseng, as a traditional herb in Asian countries, has been used for thousands of years and contains a variety of active ingredients with numerous benefits, but further high-quality evidence is needed to prove its medicinal value.
JOURNAL OF GINSENG RESEARCH
(2021)
Article
Nutrition & Dietetics
Zhi-man Li, Zi-jun Shao, Di Qu, Xiao-hui Huo, Mei Hua, Jian-bo Chen, Yu-shun Lu, Ji-Yue Sha, Shan-shan Li, Yin-shi Sun
Summary: This study investigated the effect of amino acids and processing methods on the conversion of ginsenosides in American ginseng to rare ginsenosides, as well as their antitumour effects. The results showed that aspartic acid was the best catalyst, and the thermal extraction method had the best effect. AGS-H synergistic with CTX or CTX/2 could improve the efficacy and reduce toxicity.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemical Research Methods
Jihua Liu, Qinghai Dong, Guangguang Du, Jia Wang, Yang An, Jiayin Liu, Jun Su, Hongliu Xie, Jianyuan Yin
Summary: This study identified differential metabolites in plasma after oral administration of Panax ginseng and American ginseng, and used network pharmacology to analyze the relationship between these metabolites and the different biological activities of the two herbs.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Rong-Rong Zhou, Jian-Hua Huang, Dan He, Zi-Yang Yi, Di Zhao, Zhao Liu, Shui-Han Zhang, Lu-Qi Huang
Summary: In this study, a green and effective extraction method combining deep eutectic solvents and aqueous two-phase systems was proposed to extract ginsenosides and polysaccharides from American ginseng. The method avoids the use of organic reagents and allows for solvent recovery and reuse.
Article
Plant Sciences
Md. Jahirul Islam, Byeong Ryeol Ryu, Md. Obyedul Kalam Azad, Md. Hafizur Rahman, Md. Soyel Rana, Jung-Dae Lim, Young-Seok Lim
Summary: The study demonstrated that exogenously applied putrescine can enhance salt tolerance in Panax ginseng by promoting the accumulation of certain substances and increasing antioxidant enzyme activity. Additionally, the effects of putrescine on ginsenoside levels in ginseng vary depending on the type of ginsenosides and plant parts. These findings provide valuable insights for utilizing putrescine to modulate salt tolerance and ginsenoside content in ginseng sprouts.
Review
Medicine, General & Internal
H. W. Lee, M. S. Lee, T. H. Kim, T. Alraek, C. Zaslawski, J. W. Kim, D. G. Moon
Summary: The study on the effects of ginseng on erectile dysfunction found that ginseng has trivial effects on erectile function and satisfaction with intercourse, with slight improvement in self-reported ability to have intercourse but little impact on adverse events. The comparison with other agents for treating erectile dysfunction, such as phosphodiesterase-5 inhibitors, was not addressed in this study.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Article
Agricultural Engineering
Xinyao Su, Qiang Xue, Mengchu Sun, Jiarou Liu, Ming Hung Wong, Caixia Wang, Shilin Chen
Summary: The study achieved co-production of polysaccharides, ginsenosides and succinic acid from Panax ginseng residue, demonstrating the potential economic value of this industrially feasible process through multi-step processing and transformation.
BIORESOURCE TECHNOLOGY
(2021)
Article
Plant Sciences
A. B. Cunningham, J. A. Brinckmann, R. N. Kulloli, U. Schippmann
JOURNAL OF ETHNOPHARMACOLOGY
(2018)
Review
Plant Sciences
J. A. Brinckmann, W. Luo, Q. Xu, X. He, J. Wu, A. B. Cunningham
JOURNAL OF ETHNOPHARMACOLOGY
(2018)
Review
Plant Sciences
A. B. Cunningham, J. A. Brinckmann, Y. -F. Bi, S. -J. Pei, U. Schippmann, P. Luo
JOURNAL OF ETHNOPHARMACOLOGY
(2018)
Review
Plant Sciences
T. Brendler, J. A. Brinckmann, U. Schippmann
JOURNAL OF ETHNOPHARMACOLOGY
(2018)
Editorial Material
Plant Sciences
A. B. Cunningham, J. A. Brinckmann, X. Yang, J. He
JOURNAL OF ETHNOPHARMACOLOGY
(2019)
Article
Plant Sciences
A. B. Cunningham, H. L. Li, P. Luo, W. J. Zhao, X. C. Long, J. A. Brinckmann
JOURNAL OF ETHNOPHARMACOLOGY
(2020)
Review
Plant Sciences
Wendy L. Applequist, Josef A. Brinckmann, Anthony B. Cunningham, Robbie E. Hart, Michael Heinrich, David R. Katerere, Tinde van Andel
Review
Neurosciences
Thomas Brendler, Josef A. Brinckmann, Ulrich Feiter, Nigel Gericke, Lucy Lang, Olga N. Pozharitskaya, Alexander N. Shikov, Michael Smith, Ben-Erik van Wyk
Summary: Modern regulatory systems create barriers for traditional herbal medicines to enter international markets, aiming to ensure consumer safety and product quality. This unintentional regulatory favoritism may hinder innovation and create economic hurdles for botanicals from developing countries to enter global health product markets.
CURRENT NEUROPHARMACOLOGY
(2021)
Review
Plant Sciences
J. A. Brinckmann, A. B. Cunningham, David E. Harter
Summary: The study evaluated the conservation status, harvesting, and trade levels of Rhodiola rosea, as well as the substitution of other species. Global demand for R. rosea has been increasing in the 21st century, while wild populations in commercial harvesting areas continue to decrease, leading to conservation issues and reduced supply.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Plant Sciences
A. B. Cunningham, J. A. Brinckmann, D. E. Harter
Summary: Griffonia simplicifolia seeds are high in 5-HTP, used for treating depression, leading to increasing trade demands. Wild populations in West Africa face challenges such as rapid loss of habitats, declining populations, and negative impacts of invasive species.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Plant Sciences
Josef A. Brinckmann, Wolfgang Kathe, Karin Berkhoudt, David E. Harter, Uwe Schippmann
Summary: This study provides an updated estimation of medicinal and aromatic plant species and their conservation status in commercial cultivation globally. The results show a significantly higher number of cultivated species than previously estimated, and highlight the potential consequences of overexploitation on the domestication of these plants.
Article
Chemistry, Applied
Bharathi Avula, Ji-Yeong Bae, Amar G. Chittiboyina, Yan-Hong Wang, Mei Wang, Jianping Zhao, Zulfiqar Ali, Josef A. Brinckmann, Jing Li, Charles Wu, Ikhlas A. Khan
Summary: This study used chemical profiling and statistical analysis to differentiate the roots of different Glycyrrhiza species and to assess the quality of licorice products. The results showed that different species of licorice have distinct chemical compositions, which can be used as markers for identification. The study also demonstrated the usefulness of these tools for quality assessment of licorice products.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Review
Pharmacology & Pharmacy
Maria Monagas, Thomas Brendler, Josef Brinckmann, Steven Dentali, Stefan Gafner, Gabriel Giancaspro, Holly Johnson, James Kababick, Cuiying Ma, Hellen Oketch-Rabah, Pilar Pais, Nandu Sarma, Robin Marles
Summary: The current good manufacturing practice for dietary supplements emphasizes the establishment of quality parameters for each component. Certificates of analysis provide scientifically valid analysis results, but there are descriptive data that cannot be verified by analysis methods. Plant to Extract ratios, often used to measure extract strength, do not fully describe the composition of botanical extracts.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
T. Brendler, J. A. Brinckmann, M. Daoust, H. He, G. Mase, K. Steffan, M. Williams
Summary: Herbal tea is widely used in traditional and alternative medicine, and incorporating botanical extracts into tea formulations is of vital interest to manufacturers. However, there are challenges in formulating with botanical extracts, such as sourcing high-quality ingredients and developing suitable quality control methods. This article shares experiences in overcoming these challenges and provides guidance to the extract industry.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Integrative & Complementary Medicine
Josef A. Brinckmann, Anthony B. Cunningham, David E. V. Harter
Summary: The study evaluated the conservation status, cultivation levels, and threats to wild populations of Rhodiola sachalinensis, finding that despite increasing cultivation, the species is facing challenges in its natural habitat due to climate change, habitat loss, and overexploitation. Conservation efforts and monitoring of wild populations are still crucial for the future use of this species.
WORLD JOURNAL OF TRADITIONAL CHINESE MEDICINE
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
